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Method for preparing naringenin and apigenin by converting exocarpium citrus grandis leaf flavone by using microorganism

A technology of microbial transformation and naringenin, applied in the direction of fermentation, can solve the problems of complex process and large amount of iodine, and achieve the effects of mild conditions, high conversion rate and avoiding pollution problems.

Inactive Publication Date: 2017-05-10
GUANGDONG UNIV OF PETROCHEMICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using naringin as a raw material can only be prepared through two-step reactions, the process is complicated, and the amount of iodine is relatively large

Method used

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  • Method for preparing naringenin and apigenin by converting exocarpium citrus grandis leaf flavone by using microorganism
  • Method for preparing naringenin and apigenin by converting exocarpium citrus grandis leaf flavone by using microorganism
  • Method for preparing naringenin and apigenin by converting exocarpium citrus grandis leaf flavone by using microorganism

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Pick the young leaves of tangerine red, wash them, and dry them in the sun or in an oven at 60°C to constant weight, and crush them through an 80-mesh sieve for later use. Take 100 g of leaf powder, moisten it with distilled water, stir it well, put it in a petri dish with a diameter of 12 cm, open it, and put it in an incubator with a temperature of 25 ° C and a humidity of 85% for 72 hours. Bacteria or molds that grow naturally strains. Grind 150g of boiled potatoes into a slurry, add 50g of citrus fruit powder, shake well, and dilute to 1000mL with water to make potato seed fungus culture medium. Inoculate 2 g of the above strains (including culture medium) into 500 mL of potato liquid seed fungus culture medium, and incubate at 30°C and 180 rpm for 60 h to obtain a seed fungus culture solution.

[0029] Take 1000g of citrus red leaf powder and add 3000mL of water, stir well to form a transformation medium, add 250mL of the above-mentioned cultured seed bacteria sol...

Embodiment 2

[0034]Pick the young leaves of tangerine red, wash them, and dry them in the sun or in an oven at 60°C to constant weight, and crush them through an 80-mesh sieve for later use. Take 100 g of leaf powder, add 200 mL of distilled water, moisten it, stir well, divide it into petri dishes with a diameter of 12 cm, open it, and culture it in an incubator with a temperature of 25 °C and a humidity of 95% for 96 hours to obtain seed fungi. Grind 150g of boiled potatoes into a slurry, add 50g of citrus fruit powder, shake well, and dilute to 1000mL with water to make potato seed fungus culture medium. Inoculate 3 g of the above strains (including culture medium) into 500 mL of potato liquid seed fungus culture medium, and incubate at 35°C and 220 rpm for 48 h to obtain a seed fungus culture solution.

[0035] Take 1000g of citrus red leaf powder and add 3500mL of water, stir well to form a transformation medium, add 200mL of the above-mentioned cultured seed bacteria solution, stir e...

Embodiment 3

[0040] Pick the young leaves of tangerine red, wash them, and dry them in the sun or in an oven at 60°C to constant weight, and crush them through an 80-mesh sieve for later use. Take 100 g of leaf powder and add 200 mL, moisten it, stir well, divide it into petri dishes with a diameter of 12 cm, open it, and culture it in an incubator with a temperature of 35 °C and a humidity of 95% for 84 hours to obtain seed fungi. Grind 150g of boiled potatoes into a slurry, add 50g of citrus fruit powder, shake well, and dilute to 1000mL with water to make potato seed fungus culture medium. Inoculate 3 g of the above strains (including culture medium) into 500 mL of potato liquid seed fungus culture medium, and incubate at 40°C and 250 rpm for 72 h to obtain a seed fungus culture solution.

[0041] Take 1000g of citrus red leaf powder and add 4000mL of water, stir well to form a transformation medium, add 250mL of the above-mentioned cultured seed bacteria solution, stir evenly, divide i...

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Abstract

The invention discloses a method for preparing naringenin and apigenin by converting exocarpium citrus grandis leaf flavone by using microorganism. The method comprises the steps of making bacteria and mold naturally growing in exocarpium citrus grandis material into suspension liquid, inoculating the suspension liquid into a potato liquid medium for culturing to acquire seed bacterium culturing solution, inoculating seed bacteria into a conversion medium for culturing, and performing high performance liquid chromatography. A result shows that the quantities of the naringenin and the apigenin are improved above ten times; and the pure naringenin and apigenin are respectively acquired by performing organic solvent extraction and macroporous resin separation and purification on conversion liquid. According to the method provided by the invention, respective extraction of naringin and rhoifolin is not needed, and the microorganism is directly used for converting naringin and rhoifolin substrates synchronously existing in exocarpium citrus grandis leaves. The method is simple and easy to operated, a pollution problem caused by acid hydrolysis is also avoided; as the strain is taken from elements naturally growing on exocarpium citrus grandis branches or fruits, so that the method has the advantages of strong selectivity and high conversion rate, and purities of the naringenin and apigenin prepared according to the method can be more than 98%.

Description

technical field [0001] The invention relates to the technical field of microbial transformation and extraction of plant active ingredients, in particular, it provides a method for transforming and preparing naringenin and apigenin from raw materials of orange red leaves. Background technique [0002] Naringenin is a dihydroflavonoid compound, it is the aglycone of naringin, white needle crystal, melting point 260°C, soluble in ethanol, ether and benzene, almost insoluble in water, molecular formula is C15H12O5, molecular weight is 272.25, structural formula As shown in formula 1. It has antibacterial, anti-inflammatory, anti-oxidant, cough-relieving, expectorant, blood-lipid-lowering, anti-tumor, choleretic, prevention and treatment of liver diseases, inhibition of platelet aggregation, etc., and can be widely used in medicine, food and other fields. [0003] [0004] At present, there are two main methods for preparing naringenin: 1. Extraction method, that is, extracti...

Claims

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Application Information

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IPC IPC(8): C12P17/06
Inventor 刘成有肖丹韩寒冰韦明肯黄瑶刘杰凤
Owner GUANGDONG UNIV OF PETROCHEMICAL TECH
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