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Preparation method of ulva fasciata polysaccharide-tween 20 compounded beta-carotene emulsion

A carotene and splinter Ulva technology, which is applied in the directions of polysaccharide/gum-containing food ingredients, pharmaceutical formulations, and emulsion transportation, can solve the problems of unstable emulsification of the system, and solve problems such as difficulty in being absorbed by the human body, increase emulsifying ability and Stability, small effect of steric hindrance

Active Publication Date: 2017-05-10
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when the polysaccharide of Ulva schistolii is used at a moderate concentration, the effect of droplet flocculation to increase the effective volume of particles (promote emulsion) is greater than the effect of increasing the viscosity of the continuous phase (inhibit emulsion), thus causing instability of emulsion in the system

Method used

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  • Preparation method of ulva fasciata polysaccharide-tween 20 compounded beta-carotene emulsion
  • Preparation method of ulva fasciata polysaccharide-tween 20 compounded beta-carotene emulsion
  • Preparation method of ulva fasciata polysaccharide-tween 20 compounded beta-carotene emulsion

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Embodiment 1

[0035] (1) Extraction of polysaccharides from Ulva lobes: Wash a certain amount of Ulva lobes, control water, dry in an oven at 60°C, and cut into pieces. Add 95% ethanol solution to immerse and degrease, and let stand overnight. Take out the degreased Ulva sliver and dry it in the sun, add distilled water according to the ratio of solid to liquid 1:10, bathe in 95℃ water for 3 hours, and repeat the extraction 3 times. After hot water extraction, cool and stand, freeze and centrifuge. The supernatant was concentrated by rotary evaporation to a certain volume. Add 95% ethanol solution until the final volume fraction of ethanol is 80%, let it stand overnight, centrifuge at 8000r / min at low temperature, take the precipitate, add a small amount of distilled water to dissolve, completely evaporate the residual alcohol by rotary evaporation, concentrate to a certain volume, and remove it by Sevage method. For protein, the operation was repeated 5 times, and the rotary evaporation ...

Embodiment 2

[0038](1) Extraction of polysaccharides from Ulva lobes: Wash a certain amount of Ulva lobes, control water, dry in an oven at 60°C, and cut into pieces. Add 95% ethanol solution to immerse and degrease, and let stand overnight. Take out the degreased Ulva sliver and dry it in the sun, add distilled water according to the ratio of solid to liquid 1:10, bathe in 95℃ water for 3 hours, and repeat the extraction 3 times. After hot water extraction, cool and stand, freeze and centrifuge. The supernatant was concentrated by rotary evaporation to a certain volume. Add 95% ethanol solution until the final volume fraction of ethanol is 80%, let it stand overnight, centrifuge at 8000r / min at low temperature, take the precipitate, add a small amount of distilled water to dissolve, completely evaporate the residual alcohol by rotary evaporation, concentrate to a certain volume, and remove it by Sevage method. For protein, the operation was repeated 5 times, and the rotary evaporation w...

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Abstract

The invention discloses a preparation method of an ulva fasciata polysaccharide-tween 20 compounded beta-carotene emulsion. The method comprises steps as follows: (1) ulva fasciata polysaccharide and tween 20 are dispersed in water in the mass ratio being 3: 2, and an ulva fasciata polysaccharide-tween 20 compounded solution with the mass concentration of 3%-4%, namely, an aqueous phase, is obtained; (2) an oil phase is obtained by dissolving beta-carotene in edible vegetable oil, the mass percentage of beta-carotene is 0.3%-0.5%, and the temperature is kept at 60 DEG C or below in the dissolution process; (3) the oil phase obtained in step (2) and the aqueous phase obtained in step (1) are mixed in the mass ratio being 1: (10-20) for shear emulsification, high-pressure homogenization is performed in an ice-water bath, and the beta-carotene emulsion is obtained. The method is convenient to operate, the cost is low, and the beta-carotene emulsion prepared with the method has high bioavailability, good stability and high biosafety.

Description

[0001] (1) Technical field [0002] The invention relates to the technical field of processing and application of medicine, food and health food, and relates to a preparation method of Ulva schistophora-Tween 20 compound beta-carotene emulsion. [0003] (2) Background technology [0004] With the continuous development of the economy and the improvement of human living standards, human beings are paying more and more attention to health. They expect to obtain more various nutrients needed in daily life in a simpler way to meet the daily needs of the human body and the human body. For the prevention of disease, functional food came into being and gained great attention. β-carotene, as a natural pigment, antioxidant and efficient vitamin A precursor, can be used as a safe additive in the food field, but β-carotene has poor solubility, instability and easy oxidative decomposition, etc. characteristics, which limit its application in food. To overcome this problem, β-carotene is ...

Claims

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Application Information

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IPC IPC(8): A23L33/125A23L33/105A23L29/10A61K9/107A61K47/36A61K47/26A61K31/015
CPCA23V2002/00A61K9/107A61K31/015A61K47/26A61K47/36A23V2200/222A23V2250/211A23V2250/51A23V2300/26
Inventor 邵平邱镪朱岳琼孙培龙
Owner ZHEJIANG UNIV OF TECH
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