Dog mesenchymal stem cell injection and preparation method and application thereof

A technology of stem cells and injections, which is applied in the field of biomedicine, can solve the problems of few and few applications of stem cells, and achieve the effect of good curative effect, high stability and safe use

Inactive Publication Date: 2017-03-29
FOSHAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But at present, there are not many studies on stem cells in dogs, and th...

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  • Dog mesenchymal stem cell injection and preparation method and application thereof
  • Dog mesenchymal stem cell injection and preparation method and application thereof
  • Dog mesenchymal stem cell injection and preparation method and application thereof

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Embodiment Construction

[0036] The preferred embodiments of the present invention will be described below in conjunction with the accompanying drawings. It should be understood that the preferred embodiments described here are only used to illustrate and explain the present invention, and are not intended to limit the present invention.

[0037] 1. Preparation of canine bone marrow-derived mesenchymal stem cells:

[0038] (1) Collection of bone marrow

[0039] Routine depilation, disinfection, draping of dogs after anesthesia, puncture with 16-gauge bone marrow puncture needle on the left or right iliac area under aseptic operation, and use pre-prepared anticoagulant (3000U heparin) Draw 3ml of bone marrow fluid into a 15ml centrifuge tube. Take 3ml of bone marrow fluid, add 3ml of PBS buffer containing double antibody (double dilution) to make 6ml of bone marrow dilution.

[0040] (2) Separation by Ficoll liquid density gradient centrifugation

[0041] Take 3ml of 1.077g / ml Ficoll solution, add it ...

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Abstract

The invention provides dog mesenchymal stem cell injection and a preparation method and application thereof. The dog mesenchymal stem cell injection comprises dog mesenchymal stem cells, heparin calcium, dog blood albumin and an electrolyte solution. The mesenchymal stem cells from dog bone marrow are utilized ingeniously, and combined with other compositions to be prepared into an injection form, namely the dog mesenchymal stem cell injection. The traditional therapeutic method for a dog skin tissue injury is broken. A stem cell therapy is brought in the field of pet medical treatment, the dog mesenchymal stem cell injection has the advantages that stability is high, the curative effect is good, usage is safe, long-term preservation and transportation are achieved, no toxic and side effect exists, and the foundation is laid for large-scale clinic use.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a canine mesenchymal stem cell injection and a preparation method and application thereof. Background technique [0002] With the improvement of people's living standards, the acceleration of urbanization, the reduction of family size and the aggravation of population aging, pet dogs have gradually become an important companion in people's lives, and the number of breeding is increasing. Currently, the incidence rate of dog skin diseases is relatively high, including some accidental burns, bruises, surgical operations, etc. causing large area of ​​dog skin trauma. At present, due to the poor treatment effect, it greatly affects the ornamental and economic value of dogs. [0003] Skin trauma generally refers to skin tissue damage caused by various factors and possible systemic reactions. Although the wound type and injury degree of skin trauma are different, the healing me...

Claims

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Application Information

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IPC IPC(8): A61K38/38A61K47/36A61K9/10A61P17/02A61K35/28
CPCA61K35/28A61K9/0019A61K9/10A61K38/385A61K47/36A61K2300/00
Inventor 张贝莹王丙云李东升罗冬章麦海涛陈祥浪郭彦陈志胜陈胜锋计慧琴冼琼珍
Owner FOSHAN UNIVERSITY
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