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Toona sinensis endophytic fungus 56-50 and its secondary metabolites, preparation method and application

A technology of secondary metabolites and endophytic fungi, applied in the field of microorganisms, can solve problems such as difficult treatment, and achieve the effect of inhibiting bacterial activity, good inhibitory effect, and low cost

Active Publication Date: 2019-05-24
HENAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to overcome the existing difficulties in the treatment of Pseudomonas aeruginosa and / or Proteus infection, the object of the present invention is to provide a Toona sinensis endophytic fungus 56-50 and its secondary metabolites, preparation method and application

Method used

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  • Toona sinensis endophytic fungus 56-50 and its secondary metabolites, preparation method and application
  • Toona sinensis endophytic fungus 56-50 and its secondary metabolites, preparation method and application
  • Toona sinensis endophytic fungus 56-50 and its secondary metabolites, preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0043] 1. Isolation and Screening of Toona sinensis Endophytic Fungi

[0044] Under aseptic operating conditions, the Chinese toon stems were soaked in 75v% ethanol, 1wt% sodium hypochlorite of available chlorine, and 75v% ethanol for 1 min each, then rinsed with sterile water for 3 times, and dried with sterile absorbent paper; Cut it into 8mm pieces, put it on the PDA plate medium, 4 roots per plate, cultivate at 28°C until the end of the stem grows hyphae, transfer the mycelium to a new PDA plate medium, and culture it by streaking, 5d After isolation and purification, a single colony was obtained, and then transferred to a PDA slant medium test tube. After 5 days of constant temperature cultivation at 28°C, the endophytic fungus 56-50 of Toona sinensis was obtained, stored in a refrigerator at 4°C, and used for later use. Preserved in China General Microorganism Culture Collection Management Center, preservation number: CGMCC No.12980.

[0045] 2. Identification of Toona ...

Embodiment 2

[0079] It is basically the same as Example 1, except that this example completes the fermentation, preparation and antibacterial activity determination of the secondary metabolites of the strain according to the following main steps.

[0080] 1-2 steps are the same as in Example 1.

[0081] 3. Small-scale fermentation of the strain:

[0082] Inoculate the Toona sinensis endophytic fungus 56-50 stored in the refrigerator at 4°C on the PDA plate medium, activate and culture in the incubator at 28°C for 4 days, then inoculate into the Erlenmeyer flask containing 200mL PDA liquid fermentation medium, and let it stand at room temperature Fermentation culture 7d.

[0083] 4. Preparation of strain secondary metabolites:

[0084] After the fermentation of the strains is completed, the fermentation culture liquid of the strain 56-50 is first crushed with a homogenizer mixer, and then filtered through a Buchner funnel to obtain the fermentation liquid and mycelia; the fermentation liq...

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Abstract

The invention belongs to the technical field of microbes, and discloses a Toona sinensis endophytic fungus 56-50. spore Alternaria mali ; Preservation unit: General Microbiology Center of China Microbiological Culture Collection Management Committee, preservation address: Institute of Microbiology, Chinese Academy of Sciences, No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, preservation date: September 30, 2016, preservation number: CGMCC No. 12980. The present invention isolates an endophytic fungus from the stem of Toona sinensis for the first time Alternaria mali , the secondary metabolites of the bacteria have good inhibitory effect on Pseudomonas aeruginosa and Proteus. The preparation of Toona sinensis endophyte 56-50 ( Alternaria mali ) The method for secondary metabolites is simple and easy, and the cost is low.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a Chinese toon endophytic fungus 56-50 and its secondary metabolites, preparation method and application. Background technique [0002] Pseudomonas aeruginosa is an opportunistic pathogen and one of the main pathogens of nosocomial infection. Patients suffering from metabolic diseases, blood diseases and malignant tumors, as well as patients after surgery or certain treatments are susceptible to infection with this bacteria. Infection lesions caused by this bacteria can lead to hematogenous dissemination, resulting in bacteremia and sepsis. Infection with Pseudomonas aeruginosa after burns can cause death. [0003] Proteus is also an opportunistic pathogen, which is widely distributed in nature, such as soil, water, garbage, spoilage organic matter, and human or animal intestines, and can cause various infections. Common infections include respiratory tract ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P1/02A61K36/06A61P31/04C12R1/645
CPCA61K36/06C12P1/02C12N1/145C12R2001/645
Inventor 王赵改王晓敏赵洪源史冠莹赵守涣杨慧张乐
Owner HENAN ACAD OF AGRI SCI
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