Quick detection kit for chicken-origin ingredient in food and feed and application of quick detection kit

Abstract

The invention provides a quick detection kit for a chicken-origin ingredient in food and feed and relates to the technical field of biological species identification. A general chicken-origin internal standard gene Actb is screened first; a nucleotide sequence of the gene Actb is as shown as SEQ ID NO.1 (Sequence Identifier Number 1); the gene Actb is located on a chromosome; single copy is easy to quantify; and the gene Actb has a constant copy number in chicken species, without allelic variation and can serve as a target gene for chicken-origin identification. LAMP amplification primers are designed by taking the gene as a target sequence; nucleotide sequences of the LAMP amplification primers are as shown as SEQ ID NO. 2-5; the LAMP amplification primers and an LAMP reaction solution constitute the detection kit; the detection kit can detect the chicken-origin ingredient in the food and the feed quickly and sensitively, and the sensitivity reaches 10pg. The kit is simple in use method, low in cost, high in specificity and very applicable to field real-time detection and has a wide application prospect in animal-origin food surveillance and meat product adulteration defense, and a reaction result is easy to observe.

Description

technical field [0001] The invention relates to the technical field of biological species identification, in particular to a loop-mediated isothermal amplification technique (LAMP) kit for detecting chicken source components in food and feed. Background technique [0002] With the development of social economy and product technology processing, many foods are illegally added with various chemical raw materials, additives, hormones, etc., and even many merchants carry out food adulteration behaviors or directly substitute inferior ones for high profits, which seriously endangers the The interests and health of consumers, so the accurate identification of food types is very important. [0003] No matter at home or abroad, chicken is the main meat for daily consumption, and its importance is self-evident. In Europe, chicken occupies the main market, supermarkets supply 60% of processed chicken products, and turkey is widely consumed. In China, chicken is the traditional poult...

AI Technical Summary

Problems solved by technology

At present, the adulteration detection technology of meat products at home and abroad is mostly based on the design of specific primers for the genes on the mitochondria to perform real-time fluorescent quantitative PCR amplification. Since the mitochondrial genes are multi-copy genes, their detection sensitivity is high, but at the same time it is difficult to distinguish due to sales, transportation Confusion between unintentional cross-contamination and intentional illegal additions

In addition, the concentration of high-copy mitochondrial DNA cannot correspond to the concentration of genomic DNA, so it is impossible to perform accurate quantitative analysis on samples. At the same time, due to the high homology of mitochondrial genes, it is difficult to achieve qualitative detection by ordinary PCR

Therefore, this method can only realize the screening and identification of meat adulteration by means of fluorescent quantitative PCR

Images