Silk fibroin biological material compounded with epidermal growth factor and preparation method of silk fibroin biological material

A technology of epidermal growth factor and silk fibroin, which is applied in the field of biomedicine, can solve the problems of silk fibroin, such as micro-effect, and achieve the effect of simple preparation, good biocompatibility, and degradable wound healing

Inactive Publication Date: 2017-02-15
AOMEI MEDICAL SUPPLIES CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among the existing patents, most of them use silk fibroin, sodium alginate, gelatin and other materials to make hydrogel dressings or sponge dressings, and silk fibroin plays little role in it.

Method used

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  • Silk fibroin biological material compounded with epidermal growth factor and preparation method of silk fibroin biological material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] This embodiment provides a method for preparing a silk fibroin biomaterial compounded with epidermal growth factor, as shown in the figure, comprising the following steps:

[0026] S101: degumming;

[0027] Put 30g silkworm raw silk into 12L containing Na 2 CO 3 (2.12g / L) boiling water solution and boiled for 30mins, stirring with a glass rod uninterruptedly during cooking to improve the degumming rate. After cooking, rub the cooked degummed silk with deionized water to remove the residual sericin. Put the fibroin fiber in a fume hood to ventilate and dry.

[0028] S102: Dissolving dialysis;

[0029] Weigh 15 g of the above-prepared degummed silk and put it into 100 mL of lithium bromide (LiBr) solution with a concentration of 9.3 mol / L for dissolving treatment, and dissolve at 60° C. for 4 hours during the dissolving treatment. The solution was stirred hourly with a glass rod to ensure complete dissolution of the silk fibers.

[0030] Carry out dialysis treatment...

Embodiment 2

[0044] This example provides a silk fibroin biomaterial compounded with epidermal growth factor, the silk fibroin biomaterial compounded with epidermal growth factor, silk fibroin and epidermal growth factor, prepared by the preparation method of the first embodiment above.

[0045] Biomaterials made of silk fibroin and epidermal growth factor are degradable and have the effect of promoting wound healing, so the material can be used to fill the inside of hole-shaped wounds, and then wrap the wound dressing on the outside to promote wound healing.

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Abstract

The invention provides a silk fibroin biological material compounded with the epidermal growth factor and a preparation method of the silk fibroin biological material. Silk fibroin is a natural high polymer material extracted from fibroin of silk, has good biocompatibility, is non-toxic to the body, has no sensitization and stimulation effects, and is partially biodegradable; the epidermal growth factor belongs to active substance in the human body, is peptide active substance composed of 53 amino acid residues, and can stimulate the tyrosine phosphorylation of receptors of the epidermal growth factors, so that the effect of repairing hyperplasia can be achieved, therefore, the silk fibroin biological scaffold prepared by adopting silk fibroin and the epidermal growth factor is degradable, and has the function of promoting the wound healing, and in addition, the operation is simple during the preparation of the silk fibroin biological scaffold.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a silk fibroin biomaterial compounded with epidermal growth factor and a preparation method thereof. Background technique [0002] As a human organ, the skin has the functions of retaining moisture, breathing and preventing bacterial invasion. Once the dermis is damaged, it cannot be completely regenerated. In clinical practice, autologous, allogeneic or xenograft skin grafts are usually used to repair large areas of deep skin damage. However, these treatments will cause new trauma to the donor on the one hand. At present, polymer biomaterials used in tissue repair include synthetic polymer materials and natural polymer materials. Synthetic polymer materials are mainly aliphatic polyester materials such as polylactic acid, although they are basically the same in terms of mechanical properties and degradability. It can meet the application of scaffold materials, but there are...

Claims

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Application Information

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IPC IPC(8): A61L27/60A61L27/54A61L27/22A61L27/58
CPCA61L27/60A61L27/227A61L27/54A61L27/58A61L2300/252A61L2300/414C08L89/00
Inventor 崔金海刘雅文柯晓欢曹孟杰
Owner AOMEI MEDICAL SUPPLIES CO LTD
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