Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Applications of lectin-modified anchovy antibacterial peptide liposome in inhibition of Listeria monocytogenes and biofilm thereof

A monoproliferative Listeria monocytogenes, biofilm technology, applied in application, food science, food preservation, etc., can solve problems such as food contamination, and achieve the effect of targeted control

Active Publication Date: 2017-02-01
QINGDAO AGRI UNIV
View PDF1 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the problem of food contamination caused by Listeria monocytogenes and its biofilm in the field of food processing and storage, and provide a lectin-modified anchovy antimicrobial peptide liposome that can inhibit Listeria monocytogenes and its biofilm. Uses in Biofilms

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Applications of lectin-modified anchovy antibacterial peptide liposome in inhibition of Listeria monocytogenes and biofilm thereof
  • Applications of lectin-modified anchovy antibacterial peptide liposome in inhibition of Listeria monocytogenes and biofilm thereof
  • Applications of lectin-modified anchovy antibacterial peptide liposome in inhibition of Listeria monocytogenes and biofilm thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: Preparation of lectin-modified anchovy antimicrobial peptide liposomes

[0020] Phosphatidylethanolamine (600 mg) was dissolved in 60 mL chloroform, 940 mg glutaric anhydride and 150 μL pyridine were added to react at 20 ℃ for 5 h. The reaction solution is separated by a silica gel column to obtain glutarylphosphatidylethanolamine. Take 10 mg of freeze-dried glutarylphosphatidylethanolamine and disperse in 150 μL, add 2 mL of 0.15 mol / L sodium chloride, and sonicate for 15 s. Adjust the pH of the reaction solution to 3.5, add 20 mg of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide, and shake it evenly. After reacting for 5 min, add 1 mL of 20 mg / mL lectin in PBS solution and react at 4 ℃ for 2 h. The product was dialyzed with flowing deionized water for 24 hours, and then dialyzed with PBS buffer for 48 hours, and then frozen to obtain lectin-modified phosphatidylethanolamine. Dissolve appropriate amount of phosphatidylethanolamine lipid and cholesterol in ch...

Embodiment 2

[0021] Example 2: Determination of the minimum inhibitory concentration and minimum bactericidal concentration of Lectin-modified anchovy peptide liposomes against Listeria monocytogenes

[0022] The lectin-modified insulin liposome solution was filtered through a 0.22 μm filter and sterilized, and diluted with sterile PBS buffer. Inoculate the Listeria monocytogenes bacterial solution in a sterile TSB culture solution and incubate overnight at 37 ℃, and then resuspend the resulting bacteria to OD 600 Between 0.1 and 0.3. Add 100 μL of LTSB medium, 100 μL of test bacteria solution and 50 μL of antimicrobial peptide liposome solutions of different concentrations to a sterilized flat-bottomed 96-well plate. Incubate at 37 ℃ for 18 h. Microplate reader to measure OD in each well 600 value. In the control group, an equal amount of modified liposomes without antimicrobial peptides was used instead of the antimicrobial peptide liposome suspension. Compared with the initial value, OD...

Embodiment 3

[0023] Example 3: The bactericidal kinetics of lectin-modified anchovy peptide liposomes against Listeria monocytogenes

[0024] The Listeria monocytogenes strain was inoculated in a sterile TSB culture medium at 37°C overnight, washed 3 times with PBS buffer, and resuspended to 1.0×10 7 CFU / mL. Add lectin-modified anchovy peptide liposomes to the bacterial suspension (final concentration is 0.5MIC, 1MIC, 2MIC and 3MIC). An equal volume of PBS buffer was added to the bacterial growth control group. After mixing, incubate at 37°C, take 100 μL of bacterial suspension every 0.5 h, perform appropriate 10-fold gradient dilution, and apply 100 μL on the solid medium. Place the petri dish upside down in a biochemical incubator, incubate at 37°C for 24 hours and count. The experiment is set to 3 replicates, and the results are averaged.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses applications of a lectin-modified anchovy antibacterial peptide liposome in inhibition of Listeria monocytogenes and a biofilm thereof, and belongs to the technical field of food microorganism control. According to the present invention, the lectin-modified anchovy antibacterial peptide liposome is prepared according to the specific affinity between the lectin and the saccharide unit in the capsule extracellular polysaccharide matrix, the Listeria monocytogenes biofilm model is established, and the inhibition effect of the lectin-modified anchovy antibacterial peptide liposome on the Listeria monocytogenes and the biofilm thereof is evaluated through the sterilization curve, the colony counting and the crystal violet staining, wherein the results show that the lectin-modified anchovy antibacterial peptide liposome provides the sterilization effect on the Listeria monocytogenes, can interfere the formation of the biofilm, and can eliminate the formed biofilm, and the effect is concentration dependent, such that the lectin-modified anchovy antibacterial peptide liposome can be used for inhibiting or clearing the Listeria monocytogenes and the biofilm thereof in the food processing and storage environments.

Description

Technical field [0001] The invention belongs to the technical field of food microorganism control, and relates to the application of a lectin-modified anchovy peptide liposome in inhibiting Listeria monocytogenes and its biofilm. Background technique [0002] Listeria monocytogenes is one of the four food-borne pathogens defined by the World Health Organization. The bacteria can enter humans and animals through contaminated food, causing symptoms such as sepsis, meningitis, miscarriage, and mononucleosis, with a fatality rate of 20-30%. The United States, France, Canada and other countries have repeatedly encountered clinical disease problems caused by food contamination caused by this bacteria. Listeria monocytogenes can adhere to the surface of food or food processing equipment to form a biofilm. Biofilm is a kind of microbial cell growth mode corresponding to planktonic cells on biological or non-biological surfaces. It is a protective and adsorbing polymer matrix that secre...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A23L3/3571
CPCA23L3/3571
Inventor 唐文婷蒲传奋
Owner QINGDAO AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com