Treatment method for performing acute stress on megalobrama amblycephala
A technology of acute stress and treatment methods, applied in the field of fish farming, can solve the problems of fish death, low anti-stress ability, and large difference in stress response
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Embodiment 1
[0018] (1) Selection and breeding of material fish: In June 2015, the group head bream was selected from the Nanquan Breeding Base of the Freshwater Fishery Research Center of the Chinese Academy of Fishery Sciences. The fish was healthy and domesticated in outdoor cages for 10 days, with a weight of (139±10) g. The body length is (22±2) cm, and the water temperature is about (25±2) ℃.
[0019] (2) Contrast injection: Divide 6 net cages into two groups, one group is the experimental group, the other is the injection group, the experimental group and the control group each have 75 fish, 25 fish in each box;
[0020] The experimental group was injected intraperitoneally with cortisol solution with a mass concentration of 5 mg / mL (solvent was ethanol), and the injection volume was 2 mL / kg body weight; the control group was injected with the same dose of ethanol.
[0021] The experimental group and the control group were sampled at 0, 2, 4, 8, 10, 12, 24 and 48 hours after injecti...
Embodiment 2
[0025] (1) Selection of material fish: In August 2015, the Nanquan Base of the Freshwater Fishery Research Center of the Chinese Academy of Fishery Sciences was selected to breed pomace. ±4) cm.
[0026] (2) Breeding of material fish: temporarily raise the material fish in a recirculation culture system for 2 weeks, control the water temperature at (25±1)°C, continue to oxygenate, the photoperiod is the same as natural light, and feed commercial pellet feed at 3% of body weight , 3 times a day.
[0027] (3) Cortisol injection: The experimental fish were intraperitoneally injected with a cortisol solution (2 mL / kg body weight, concentration 5 mg / mL, solvent is alcohol), and recorded as 0h after injection. Samples were taken at 0h, 2h, 4h, 8h, 12h, 24h, and 5d time points, and 5 fish were taken each time.
[0028] (4) Blood collection: Before sampling, the fish were anesthetized with 175 mg / L MS-222, and blood was collected from the tail vein. The same method as in Example 1 ...
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