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Application of heterorhabditis bacteriophora to biological control of underground insects of greenhouse vegetables

A Heterobacterium nematode, biological control technology, applied in the fields of application, horticulture, botanical equipment and methods, etc., can solve the problems of single control method and means, few research results, narrow application of pathogenic nematodes, etc., and achieve broad application prospects Effect

Active Publication Date: 2017-02-01
THE INST OF MICROBIOLOGY XINJIANG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, my country has made some research progress in the practical application of entomopathogenic nematodes, but compared with foreign countries, there are still many shortcomings: (1) There are no entomopathogenic nematode products that can be used for industrial production in China, and the research results related to this aspect Too few; (2) The application area of ​​pathogenic nematodes is narrow, and it is only successfully applied in the control of a few pests; (3) The control methods and means are relatively single

Method used

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  • Application of heterorhabditis bacteriophora to biological control of underground insects of greenhouse vegetables
  • Application of heterorhabditis bacteriophora to biological control of underground insects of greenhouse vegetables
  • Application of heterorhabditis bacteriophora to biological control of underground insects of greenhouse vegetables

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The specific steps of using Heterobacterium bacteriophage in the biocontrol of vegetables are as follows:

[0026] (1) Use fresh chicken viscera or remove chicken gizzards and gallbladder, or use sheep and pig liver, add 20% w / v water, 10%-20% vegetable oil, grind and mix in a homogenizer After removing the large particles, it is evenly mixed with a 1 cm square sponge at a mass ratio of 1:8 to 1:10, and then bottled and sterilized as a medium for cultivating and propagating Heterobacterium bacteriophilus.

[0027] (2) Choose a 1.3L tissue culture polypropylene high temperature resistant tissue culture bottle, control the filling volume at 1L, so that the sponge block has room to shake, and then sterilize it by high temperature steam at 121 degrees for 30 minutes. After the sterilization is completed, wait for the solid When the temperature of the culture medium drops below 40 degrees, put the Type I commensal bacteria suspension prepared after 36 hours of culture into t...

Embodiment 2

[0030] Example 2: Isolation, identification and virulence determination of bacteriophage Heterobacterium nematode

[0031] Tenebrio molitor larvae were infected with entomopathogenic nematode infestation stage nematodes, and the symbiotic bacteria were isolated after Tenebrio molitor died. Use 75% alcohol to disinfect the surface of Tenebrio molitor, tweezers, scissors, etc. on the aseptic operation table, then clamp the head and tail of Tenebrio molitor with tweezers to expose the abdomen, cut off one of the gastropods with scissors, and remove it The body fluid containing symbiotic bacteria flowing out of the body is directly dropped on the NBTA identification medium, and evenly spread with a sterilized coating rod. After inoculation, it is placed in a 30°C incubator for cultivation. After about 48 hours, a single colony appeared on the plate, and the nascent bacteria (which can absorb blue and have a transparent circle around them) were picked out and purified.

[0032] Ex...

Embodiment 3

[0036] Embodiment 3: The pathogenicity of bacteriophage Heterobacterium nematodes to Tenebrio molitor larvae and Drosophila larvae

[0037] 1. Determination of the lethality of Heterobacterium bacteriophage to Tenebrio molitor larvae and Drosophila larvae

[0038] Tenebrio molitor larvae and Drosophila larvae were placed in a small Petri dish, two layers of filter paper were laid in the Petri dish, 1 mL of water was added, 10 larvae per dish. Dilute the infection stage larvae of Heterobacterium bacteriophage and control H.megidis (a nematode strain imported from abroad for the control of grubs) to 100 IJs / mL with sterilized water, and add 1 mL into a petri dish respectively, and each The concentration was set for 3 repetitions, and the sterile water treatment with only water was set as the control. Each treatment was cultured at 25°C, and the number of deaths was checked every 24 hours.

[0039] 2. Pathogenicity of different infection doses of Heterobacterium bacteriophage t...

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Abstract

The invention discloses application of heterorhabditis bacteriophora to biological control of underground insects of greenhouse vegetables. The heterorhabditis bacteriophora is obtained through separation, and fresh chicken internal organs are adopted or chicken gizzards and gallbladders are removed; 20%w / v of water and 10%-20% of vegetable oil are added, and the mixture is homogenized and sterilized to be used as a culture medium for culturing and breeding entomopathogenic nematodes; nematode suspension liquid with the concentration of 10000Ijs per seedling is prepared and is filled to roots of the seedlings; after application, the fatality rate of mosquito and fly larvae is 100%, and the fatality rate of large-size underground insects including grubs, mole crickets and the like is 50% or more; the heterorhabditis bacteriophora has the acting effects of no toxin and no pollution, and capability of keeping a long effect after one time of application; the heterorhabditis bacteriophora has active searching and expanding propagation capabilities, so that the heterorhabditis bacteriophora has the acting effect on underground insect larvae which are difficult to prevent and control and the acting effect is better than that of other medicines; and the heterorhabditis bacteriophora has a wide application prospect on production of green and healthy greenhouse vegetables.

Description

technical field [0001] The invention relates to the technical field of microorganism application, in particular to the technical field of utilizing a new entomopathogenic nematode and its application in vegetable biocontrol. Background technique [0002] Entomopathogenic nematodes are tiny nematodes that are obligate parasitic insects and carry symbiotic bacteria in their bodies. They carry pathogenic bacteria in their digestive tracts. This type of nematodes has an infection stage that can live independently from the host camp. When it After invading the insect host body from the insect digestive tract or body wall, the symbiotic bacteria are released from the nematode body, proliferate in the blood of the insect, and eventually cause the host insect to be infected with pathogenic bacteria and die of sepsis. The nematodes described and named in the world at present are mainly the nematodes of the Steineridae family and the Heterobradidae family. In the life history of ento...

Claims

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Application Information

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IPC IPC(8): A01G13/00A01K67/033
CPCA01G13/00A01K67/033
Inventor 詹发强龙宣杞侯敏杨蓉崔卫东布卡·欧尔娜
Owner THE INST OF MICROBIOLOGY XINJIANG ACADEMY OF AGRI SCI
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