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Preparation method of essence containing human mesenchymal stem cell factors

A technology of stem cells and essence, applied in the field of preparation of essence, can solve the problems of decreased cytokine activity, poor repair effect, low safety of use, etc., and achieves the effect of maintaining activity, promoting stem cell growth and improving recovery rate.

Inactive Publication Date: 2017-01-25
GENESIS STEMCELL REGENERATIVE MEDICINE ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are also some reports on the purification of stem cell culture supernatant and stem cell lysis, but the results are not good. For example, the sugar residue in the supernatant, the culture supernatant contains fetal bovine serum, and the activity of cytokines decreases during the concentration process. Fix poor results, low security and other issues

Method used

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  • Preparation method of essence containing human mesenchymal stem cell factors
  • Preparation method of essence containing human mesenchymal stem cell factors
  • Preparation method of essence containing human mesenchymal stem cell factors

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preparation example Construction

[0034] The preparation method of the essence containing human mesenchymal stem cell factor, the preparation method of the essence containing human mesenchymal stem cell factor is completed through the preparation of freeze-dried powder of human mesenchymal stem cell factor, preparation of solvent and mixing steps, and the steps are as follows:

[0035]a. Preparation of human mesenchymal stem cell factor freeze-dried powder: after the separation and culture of human mesenchymal stem cells, cell passage, identification of mesenchymal stem cells, collection of supernatant, concentration of supernatant and preparation of freeze-dried powder Steps to complete the preparation of human mesenchymal stem cell factor freeze-dried powder; the specific steps are as follows:

[0036] (1) Isolation and cultivation of human mesenchymal stem cells: put healthy human umbilical cord, placenta, and adipose tissue into a class 100 ultra-clean bench, after washing and disinfection, add mixed collag...

Embodiment 1

[0046] 1. Changes of cytokine concentration in stem cell supernatant before and after concentration

[0047] Growth factors represented by epidermal growth factor, vascular endothelial growth factor, and platelet-derived growth factor were detected using ELASIA kit. Carry out content and recovery rate comparison after concentration, as shown in table 1:

[0048]

[0049] Table 1

[0050] As can be seen from Table 1, the cell supernatant obtained by the combined closed-circuit ultrafiltration membrane filtration method in the present invention is concentrated about 40 times, and the recovery rate of cytokines is higher than 90%, and the platelet-derived growth factor is An important mitogenic factor, which has the ability to stimulate the division and proliferation of specific cell groups, regenerate blood vessels, promote collagen synthesis, and delay aging. Vascular endothelial growth factor can directly act on vascular endothelial cells to promote the proliferation of v...

Embodiment 2

[0069] Collection, Concentration and Lyophilization of Amnion-Derived Mesenchymal Stem Cell Supernatant

[0070] 1. Organization acquisition:

[0071] The placenta must be voluntarily donated by parturients in accordance with ethical and moral standards. The maternal HBV antigen, anti-HCV antibody, anti-HIV antibody, anti-Treponema pallidum antibody, HTLV, EB virus, HCMV and other test items are all negative, and there is no previous medical history or family history;

[0072] 2. Isolation and cultivation of mesenchymal stem cells: Take the placenta out of the preservation solution, place it flat on a sterile tray with the amniotic membrane facing up, wipe it clockwise with 75% alcohol cotton, repeat twice, and remove the placenta from the umbilical cord. Strip the sterilized amniotic membrane tissue as the center, put the amniotic membrane tissue into a 90cm sterile petri dish, and wash with normal saline containing 500 IU / ml cephalexin, 800U / ml gentamicin, and 3ug / ml amphote...

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Abstract

The invention discloses a preparation method of an essence containing human mesenchymal stem cell factors. The preparation method of the essence containing the human mesenchymal stem cell factors comprises the steps: preparation of freeze-dried powder of the human mesenchymal stem cell factors, preparation of a solvent and mixing. The preparation method obtains a high-purity and high-activity stem cell active factor concentrated solution by a cryoconcentration technique and the concentrated solution is freeze-dried to obtainfreeze-dried powder at low temperature in vacuum, so that the factor activity can be kept for a long time; and the solvent contains multiple skin nourishing and moisturizing components and a stem cell lysis solution, can well dissolve the cell factor freeze-dried powder and keeps the cell factor activity. The stem cell essence disclosed by the invention contains vascular endothelial cell growth factors, platelet derived growth factors, epidermal growth factors and other components, can promote skin regeneration, can play roles in moisturizing, tendering, whitening and repairing the skin, and has good application prospects in fields of medical beauty treatment, health protection and the like.

Description

technical field [0001] The invention relates to a preparation method of essence, in particular to a preparation method of essence containing human mesenchymal stem cell factor. Background technique [0002] Mesenchymal stem cells refer to a class of stem cell subgroups with the potential to differentiate into three germ layers. Under certain circumstances, they can theoretically be induced to differentiate into any tissue cells in the human body. Bone marrow, fat, umbilical cord and placenta, etc., are more and more concerned about their clinical application value because of their strong proliferation ability, strong differentiation ability, low immunogenicity and immune regulation function. With the deepening of research on mesenchymal stem cells, its secreted factors have become a research hotspot. Mesenchymal stem cells can secrete a variety of cytokines, which can effectively regulate body cell signal transduction, activate human stem cells, and then physiologically rep...

Claims

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Application Information

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IPC IPC(8): A61K8/99A61Q19/02A61Q19/08A61Q19/00
CPCA61K8/99A61K2800/805A61Q19/00A61Q19/02A61Q19/08
Inventor 谢再东殷鉴强王军霞
Owner GENESIS STEMCELL REGENERATIVE MEDICINE ENG CO LTD
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