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Undenatured collagen based biosurfactant with ionic liquid as reaction medium and preparation method thereof

A surfactant and ionic liquid technology, applied in the fields of beauty cosmetics and biomedicine, can solve the problems of undenatured collagen-based biosurfactant surface tension not reaching the ideal requirements, uneven dispersion, low reaction efficiency, etc., and achieve surface activity. Good, low surface tension, the effect of improving the grafting rate

Active Publication Date: 2016-11-09
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the contact reaction between collagen and long hydrophobic chains in the form of solid-liquid reaction in organic solvents in the early stage, the dispersion is uneven and the reaction efficiency is low, which has certain limitations, so that the grafting rate of long hydrophobic chains has been lower than 38%. %, resulting in the surface tension of the undenatured collagen-based biosurfactant still not reaching the ideal requirement, so increasing the grafting rate of the hydrophobic long chain is the key to reducing the surface tension of the undenatured collagen-based biosurfactant

Method used

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  • Undenatured collagen based biosurfactant with ionic liquid as reaction medium and preparation method thereof
  • Undenatured collagen based biosurfactant with ionic liquid as reaction medium and preparation method thereof
  • Undenatured collagen based biosurfactant with ionic liquid as reaction medium and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Take 0.1g of freeze-dried undenatured natural cowhide collagen and cut it into pieces in a 50mL glass beaker, add 5g of 1-ethyl-3-methylimidazolium acetate ionic liquid, keep stirring for 5h to fully dissolve, then add 1.0mL 0.003g of lauric aldehyde dissolved in dimethyl sulfoxide was reacted in an ice bath at 4°C for 10 hours. After the reaction was completed, the pH was adjusted to 9 with HCl, and then 0.01 g of succinic anhydride dissolved in 0.5 mL of dimethyl sulfoxide was slowly added. At the same time, the pH was stabilized at 9 with NaOH, and reacted in an ice bath at 4°C for 5 hours. After the reaction, HCl was added until precipitation occurred. After centrifugation, the precipitate was washed 5 times with deionized water adjusted to pH = 4.2 to 4.6 with HCl to obtain the following: 1-Ethyl-3-methylimidazolium acetate ionic liquid is the undenatured collagen-based biosurfactant grafted with laurylaldehyde and succinic anhydride as the reaction medium.

[0034...

Embodiment 2

[0036] Take 0.1g of freeze-dried undenatured natural pigskin collagen and cut it into pieces in a 50mL glass beaker, add 20g of 1-butyl-3-methylimidazolium acetate ionic liquid, keep stirring for 5h to fully dissolve, then add 1.0 Dissolve 0.01g of lauroyl chloride in mL of acetone, react in ice bath at 6°C for 10h, adjust the pH to 9.5 with HCl after the reaction, then slowly add 0.03g of malonic anhydride dissolved in 1.0mL of acetone, and stabilize the pH with NaOH React in an ice bath at 9.5 and 6°C for 5 hours. After the reaction, add HCl until precipitation occurs. After centrifugation, the precipitate is washed 5 times with deionized water adjusted to pH = 4.2 to 4.6 with HCl to obtain 1-butyl-3- Methylimidazolium acetate ionic liquid is undenatured collagen-based biosurfactant grafted with lauroyl chloride and malonic anhydride as reaction medium.

[0037] Use glycine as a standard curve, and use natural collagen as a contrast, measure the absorbance of the mixed solut...

Embodiment 3

[0039] Take 0.1g of freeze-dried undenatured natural fish skin collagen and cut it into pieces in a 50mL glass beaker, add 30g of dihydrogen phosphate choline salt ionic liquid, keep stirring for 5h to fully dissolve, then add 0.03g of dihydrogen phosphate dissolved in 0.5mL of ether Myristic anhydride, react in ice bath at 10°C for 10 hours, after the reaction, adjust the pH to 9 with HCl, then slowly add 0.05g of glutaric anhydride dissolved in 1.5mL of ether, and stabilize the pH at 9 with NaOH, at 10°C on ice React in the bath for 5 hours. After the reaction, add HCl until precipitation occurs. After centrifugation, the precipitate is washed 5 times with deionized water adjusted to pH = 4.2-4.6 with HCl to obtain nutmeg with dihydrogen phosphate choline salt ionic liquid as the reaction medium. Anhydride and glutaric anhydride graft modified undenatured collagen-based biosurfactants.

[0040] Use glycine as a standard curve, and use natural collagen as a contrast, measure ...

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Abstract

The invention discloses an undenatured collagen based biosurfactant with ionic liquid as the reaction medium and a preparation method thereof. The method is characterized by: dissolving 1 part by weight of freeze dried undenatured natural collagen in 50-300 parts by weight of ionic liquid, adding 0.003-0.3 part by weight of a long-chain hydrophobic carbonyl compound dissolved by 5-15 parts by weight of an organic solvent, carrying out reaction under 4-10DEG C ice bath for 6-10h, at the end of the reaction using acid to adjust pH to 9-10, then slowly adding 0.1-0.5 part by weight of short-chain dicarboxylic anhydride dissolved by 5-15 parts by weight of an organic solvent, at the same time using alkali to stabilize pH at 9-10, carrying out reaction under 4-10DEG C ice bath for 3-5h, at the end of reaction using acid to adjust pH till precipitate appears, then using deionized water with pH adjusted by acid to 4.2-4.6 to wash the precipitate 3-5 times, thus obtaining the neutral and water soluble undenatured collagen based biosurfactant with high hydrophobic long-chain grafting ratio and good surface activity.

Description

technical field [0001] The invention relates to an undenatured collagen-based biosurfactant using ionic liquid as a reaction medium and a preparation method thereof, belonging to the fields of beauty cosmetics and biomedicine. technical background [0002] Collagen, as the main component of the extracellular matrix, is a structural protein widely distributed in the skin, bones, tendons and other tissues of animal organisms, which plays a role in supporting organs and protecting the body, accounting for about one-third of all vertebrate body proteins one. Collagen has a relative molecular mass of 300,000 and has a typical triple helix structure. Its unique molecular structure endows it with many excellent biological properties, such as low antigenicity, biodegradability, and good biocompatibility. It is often used in Biomedicine, Functional Health Care and Cosmetics. [0003] Biosurfactant (Biosurfactant) is a substance with a certain surface activity derived from animals o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01F17/30A61K9/107A61K47/42C09K23/30
CPCA61K47/42A61K9/1075C09K23/16
Inventor 李国英李倩刘文涛
Owner SICHUAN UNIV
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