A kind of tissue culture method of southern jujube

Abstract

The invention discloses a tissue cultivating method of choerospondias axillaries. The method includes the steps of taking axillary buds of choerospondias axillaries, disinfecting outer surfaces, cultivating the axillary buds in an MS medium, screening and disinfecting the axillary buds, putting the disinfected axillary buds in an inducing medium to be cultivated at a temperature of 10 DEG C to 12 DEG C under the luminance of 1800 LX 8 hours per day for 6 weeks, shifting the axillary buds into a subculture medium to be cultivated to continue multiplication and cultivation for 4 weeks, and shifting the buds into a root medium to be cultivated to conduct rooting cultivation for 4 weeks. By means of the method, the genders of choerospondias axillaries seedlings can be controlled and cultivated in an oriented mode by recording the genders of collected female parents, the method is free of season limits, keeps the biological characteristics of original plants to the maximum extent, and can well prevent the browning in the cultivation process, the reproduction speed is high, and the rooting rate is 98% or higher.

Description

technical field [0001] The invention relates to the field of plant asexual propagation, in particular to a method for tissue culture of jujube. Background technique [0002] Southern jujube is an excellent fast-growing timber tree species in southern my country. Its wood structure is slightly thick, the heartwood is wide, light reddish brown, the sapwood is narrow, white to light reddish brown, the pattern is beautiful, and the planing surface is smooth. The material is flexible and has little shrinkage, so it can be processed into handicrafts. The fruit is sweet and sour, and can be eaten raw, brewed wine and processed jujube cake; the stone can be used as a raw material for activated carbon; the leaves can be used as green manure; the bark can also be used as a raw material for tanning materials and tannins. Distributed in Zhejiang, Fujian, Hubei, Hunan, Guangdong, Guangxi, Yunnan, Guizhou, etc. The fruit of southern jujube is rich in nutrition, contains a lot of sugar, ...

AI Technical Summary

Problems solved by technology

Sour jujube is dioecious, which makes it difficult to control the number of male and female trees during natural planting and reproduction, resulting in a low proportion of wild jujube fruit, which is not conducive to planting wild jujube as a fruit tree; therefore, the development of modern technology for seedling cultivation is used in wild jujube There is a lot to be done in breeding seedlings