Method for extracting anthocyanidin from black chokeberries

Abstract

The invention discloses a method for extracting anthocyanidin from black chokeberries. Azeotropic liquid (an EM reagent for short) of ethyl acetate and methyl alcohol is adopted as an extracting solvent for anthocyanidin in black chokeberries by replacing different ratios of ethanol solutions, both anthocyanidin and anthocyanidin aglycon can be extracted out, and the anthocyanidin extraction rate is increased; meanwhile, when the EM reagent is used as the extracting solvent for anthocyanidin, polarity is appropriate, few impurities with large polarity and few impurities with small polarity are dissolved out, column chromatography isolation and purification are not needed, the anthocyanidin yield is high, and the concentrated extracting solution can be directly subjected to spray drying or vacuum drying to form solid. Accordingly, by means of the method, industrial large-scale production is promoted, meanwhile, the production period is shortened, and the production cost is reduced.

Description

technical field [0001] The invention belongs to the technical field of forest food deep processing, and relates to a method for extracting anthocyanins from Aronia nigra. Background technique [0002] Aronia melanocarpa is a species of Aronia melanocarpa in the family Rosaceae. It is native to the northeastern United States and was introduced into my country in the 1990s. Now my country has completed the introduction, breeding, cultivation, and regional experiments. Many areas are promoting planting on a large scale. Arana berries are rich in anthocyanins, proanthocyanidins, flavonoids and other polyphenols, etc. Its anthocyanin content is more than four times that of blueberries, and proanthocyanidins are more than three times that of blueberries. The total polyphenols are more than 6 times that of blueberries. These substances have various health care and medical effects such as anti-oxidation, anti-aging, lowering blood sugar, anti-mutation and protecting eyesight. Follow...

AI Technical Summary

Problems solved by technology

Arana berries are rich in anthocyanins, proanthocyanidins, flavonoids and other polyphenols, etc. Its anthocyanin content is more than four times that of blueberries, and proanthocyanidins are more than three times that of blueberries. The total polyphenols are more than 6 times that of blueberries. These substances have various health care and medical effects such as anti-oxidation, anti-aging, lowering blood sugar, anti-mutation and protecting eyesight. Following the research on extracting anthocyanins from blueberries, people now The research work on extracting anthocyanidins and proanthocyanidins from Aronia nigrum was started, but because these works have just started, there are few literature reports on the extraction of anthocyanins from Aronia nigra. Judging from the two existing published articles on the extraction of anthocyanins from Aronia berries, both are based on the method of extracting anthocyanins from blueberries

[0003] Anthocyanins (or anthocyanins) are the general term for anthocyanins and anthocyanins. At present, the methods for extracting anthocyanins all use ethanol solutions with different mass fractions as solvents to extract anthocyanins. These methods have their advantages, but there are also disadvantages. Many shortcomings, such as "China Brewing" 2014 No. 33 published Li Mengsha's "Response Surface Method Optimizing the Ultrasonic Extraction of Aronia Aronia Anthocyanins", which proposed to use 80% volume fraction of ethanol as a solvent, The solid-liquid ratio of Aronia nigricarpa and ethanol solution was 1:10 (g / mL), the extraction temperature was 50°C, and the extraction time was 48 minutes. 3.98%) The advantage of this method is that the anthocyanin has been extracted by the auxiliary measure ultrasonic wave, the ultrasonic wave can accelerate the dissolution of the anthocyanin, shorten the extraction time, and reduce the decomposition of the anthocyanin; the disadvantage is: 1. There is no requirement for the pH value , because the size of the pH value not only determines the structural form of the anthocyanin, but also determines the stability of the anthocyanin; 2. The extraction solvent uses ethanol with a volume fraction of 80%. The coagulation force of the protein is strengthened, which is not conducive to the penetration of the solvent into the cells, and is not conducive to the dissolution of anthocyanins; 3. The aqueous solution of ethanol is used, and the temperature is higher when concentrated. Changes such as decomposition, oxidation, and polymerization are very easy to occur in an atmosphere above 45°C; 4. The author only measured the extraction rate of anthocyanins in the Aronia black fruit extract solution, and did not obtain solid anthocyanins, so this extraction The method is worthless in industrial production, because the anthocyanin is unstable in the solution, and the content of the anthocyanin in the solution decays with the passage of time, how to make the extraction solution of the anthocyanin into a solid with higher content, It is the key to the industrial production of anthocyanins. The solid anthocyanins are not only stable and easy to store, but also easy to transport and apply; 5. The extraction rate is too low, only 3.98%

Its disadvantages are: 1. The ethanol concentration is low, and the water ratio is large, which is not conducive to the extraction of anthocyanins; 2. The ethanol solution has a large water ratio, and the temperature will be high when concentrated, which causes the anthocyanin to be easily decomposed; 3. The ratio of solid to liquid If it is too large, the concentration time will be long, which will not only cause the decomposition of anthocyanins, but also the production cycle will be long, which is not conducive to industrial production; What is obtained is also an extraction solution without further processing into a solid, so industrial production is also impossible with this extraction method