Organic small molecular fluorescent probe for detecting methanol and preparation method of organic small molecular fluorescent probe

Abstract

The invention discloses a preparation method of an organic small molecular fluorescent probe for detecting methanol. The preparation method comprises the following steps: firstly, preparing 1-(p-toluenesulfonyl) imidazole; secondly, preparing the organic small molecular fluorescent probe: adding a proper amount of 4-bromotriphenylamine and the 1-(p-toluenesulfonyl) imidazole into a tetrahydrofuran solution, and then adding a catalyst consisting of copper sulfate pentahydrate and sodium ascorbate into the solution; protecting by nitrogen and stirring for 12 hours, extracting with dichloromethane, drying by using anhydrous magnesium sulfate, and performing rotary evaporation to obtain a crude product; performing column chromatographic separation on the crude product by using ethyl acetate and petroleum ether, wherein the volume ratio of the ethyl acetate to the petroleum ether is 1 to (10 to 20), and separating to obtain a solid, namely the organic small molecular fluorescent probe for detecting the methanol. The fluorescent probe disclosed by the invention has a high fluorescent selective response to the methanol; the fluorescence intensity is remarkably enhanced along with the prolonging of time; the organic small molecular fluorescent probe has the advantages of easiness in preparation, good chemical stability, high sensitivity and the like.

Description

technical field [0001] The invention belongs to the fields of chemical substance synthesis, fluorescence sensing and fluorescence analysis, and in particular relates to an organic small molecule fluorescent probe capable of identifying the methanol content in a solvent, especially the methanol content test in ethanol. The invention belongs to the fields of chemical substance synthesis, fluorescence sensing and fluorescence analysis. Background technique [0002] Methanol is a colorless, volatile liquid with an alcoholic odor. It is often used by criminals in the preparation of alcohol to make huge profits and cause serious harm. Studies have shown that the lowest dose of human oral poisoning is about 100mg / kg body weight, and oral intake of 0.3-1g / kg can be fatal. Because methanol will be oxidized into formaldehyde and formic acid (formic acid), which are more toxic than methanol, in human metabolism, drinking wine containing methanol can cause blindness, liver disease, an...

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Problems solved by technology

[0003] At present, although there are many methods for detecting methanol content, such as; gas chromatography and atomic absorption spectrometry, etc., although these methods are accurate and sensitive, the steps are lengthy, the operation is cumbersome, and the time is long, so they are not suitable for rapid determination.

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