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Magnetic fluorescent multimodal nano biological probe as well as preparation method and application thereof

A nano-bioprobe, multimodal technology, is applied to magnetic fluorescent probes to prepare biosensors. It can solve the problems of low long-term survival rate of liver cancer patients, difficult early diagnosis of tumors, long incubation period, etc., and achieve the effect of simple structure, uniform size and high affinity

Inactive Publication Date: 2016-09-07
SOUTHEAST UNIV
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Problems solved by technology

[0002] Liver cancer is one of the malignant tumors that seriously threaten human health. Although most of them are surgical resection, combined with adjuvant chemotherapy, hyperthermia and other comprehensive treatments, which have partially improved the therapeutic effect of liver cancer, the long-term survival rate of liver cancer patients is still relatively low. Its willingness is mainly due to the hidden disease of most liver cancer patients, long incubation period, and rapid tumor growth, which makes early diagnosis difficult

Method used

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  • Magnetic fluorescent multimodal nano biological probe as well as preparation method and application thereof

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preparation example Construction

[0028] Step 2, the preparation of silicon dioxide protective layer (Fe 3 o 4 @SiO 2 )

[0029]B1: Take 10mL absolute ethanol, add 100μL TEOS, shake for 1 minute and mix well.

[0030] B2: Take 10mL of absolute ethanol, add 1mL of water, then add 1mL of ammonia water, shake for 1 minute and mix well. Then add 600 μL 2mg / mL Fe 3 o 4 Solution, vortex to mix well.

[0031] Add B2 dropwise to B1 (marked as C) in an ultrasonic environment, oscillate evenly, and continue the ultrasonic reaction for 1 hour.

[0032] Step three, magnetic fluorescent probe (Fe 3 o 4 @SiO 2 (FITC))

[0033] Take 500μLA and shake for 1 minute to mix well. In an ultrasonic environment, add A drop by drop, then add 300 μLTEOS, oscillate evenly, and continue the ultrasonic reaction for 2 hours. Note that the ultrasonic temperature should not be higher than 40°C, and the entire reaction should be carried out under dark conditions.

[0034] The magnetic fluorescent probe, the silicon dioxide-embed...

Embodiment 1

[0045] The implementation process of the magnetic fluorescent multimodal probe of the present invention is as follows: figure 1 shown.

[0046] The PBS involved in this embodiment is pH 7.4, and the absolute ethanol is all high-grade pure; the paramagnetic iron oxide used is purchased from Nanjing Dongna Biotechnology Co., Ltd.; Tetraethylorthosilicate (Tetraethylorthosilicate, TEOS) and 3- Aminopropyl triethoxysilane (3-aminopropyl) triethoxysilane, APTES) was purchased from Sigma-Aldrich, USA; TLS11a Aptamer sequence was purchased from Sangon Bioengineering (Shanghai) Co., Ltd.

[0047] The magnetic fluorescent probe, the silica-embedded magnetic prepared by:

[0048] Step 1. Dissolve 20mg FITC in 10mL absolute ethanol, add 500μL APTES, mix well, and react at room temperature for 24 hours in the dark;

[0049] Step 2: Take 10mL absolute ethanol, add 100μL TEOS, oscillate, and mix well;

[0050] Step 3: Take 10mL of absolute ethanol, add 1mL of water, then add 1mL of amm...

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Abstract

The invention discloses a magnetic fluorescent multimodal nano biological probe as well as a preparation method and application thereof. The magnetic fluorescent multimodal nano biological probe specifically targets a liver-cancer cell. According to the preparation method, magnetic nano particles and fluorescent dye are co-embedded in silicon dioxide; an amino group is functionally modified by the silicon dioxide; nucleic acid aptamer and a magnetic fluorescent probe are covalently coupled and synchronously specifically target the liver-cancer cell. The magnetic nano particles are paramagnetic iron oxides of which the sizes are ten nanometers; the fluorescent dye is fluorescein isothiocyanate; the embedding medium silicon dioxide is obtained through the hydrolysis of tetraethyl orthosilicate and 3-aminopropyltriethoxysilane; the nucleic acid aptamer is TLS11a. A synthetic method of the probe is simple and convenient; materials are easily obtained; fluorescence properties are stable; a foundation is laid for the early diagnosis of a liver cancer.

Description

technical field [0001] The invention relates to the field of nano biological probes, in particular to a magnetic fluorescent probe used for preparing biosensing. Background technique [0002] Liver cancer is one of the malignant tumors that seriously threaten human health. Although most of them are surgical resection, combined with adjuvant chemotherapy, hyperthermia and other comprehensive treatments, which have partially improved the therapeutic effect of liver cancer, the long-term survival rate of liver cancer patients is still relatively low. Its willingness is mainly due to the hidden disease of most liver cancer patients, long incubation period, and rapid tumor growth, which makes early diagnosis difficult. Therefore, early precise targeted diagnosis, good biocompatibility, and low toxicity and side effects have become challenges for liver cancer and even cancer diagnosis. However, with the combination of nanotechnology and biomedicine in recent years, nanomaterials ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00A61K49/08A61K49/18A61K41/00
CPCA61K41/00A61K49/0002A61K49/0052A61K49/0093A61K49/08A61K49/1845
Inventor 刘松琴魏珍珍
Owner SOUTHEAST UNIV
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