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Method for breeding mycorrhizal seedlings by truffles and quercus acutissima

A technology of mycorrhizal seedlings and oak oaks, applied in the field of agricultural seedling cultivation, to achieve the effects of facilitating survival, improving root system vitality, and increasing infection index

Inactive Publication Date: 2016-09-07
INST OF SOIL FERTILIZER SICHUAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on the root seedling cultivation of truffle infection and inoculation with Quercus sp.

Method used

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  • Method for breeding mycorrhizal seedlings by truffles and quercus acutissima
  • Method for breeding mycorrhizal seedlings by truffles and quercus acutissima
  • Method for breeding mycorrhizal seedlings by truffles and quercus acutissima

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1 The effective inoculum amount of the bacterial agent of the present invention is 1 g, and the main root and lateral root of the plant are cut by 1 cm each.

[0049] (1) cultivate oak seedlings, the detailed steps are as follows:

[0050]Fresh oak seeds were collected in November of that year, and after water flotation, the seeds were soaked and disinfected with 20% hydrogen peroxide in a disinfectant solution for 1 hour, and the river sand was sterilized by high temperature and damp heat: 121-126°C, 2 hours; the water content of the river sand was 18%; Quercus japonica seeds were stored in sterilized and cooled river sand for 4 months at a temperature of 0-4°C and with natural humidity. Until March of the next year, sieve and select the seeds stored in sand, wash them with ultrapure water, soak the seeds with 0.2% acidic potassium permanganate solution for 1 hour, and then wash the seeds with sterile water until The color of the eluate is invisible to the nak...

Embodiment 2

[0058] Example 2 The effective inoculum amount of the bacterial agent of the present invention is 2 g, and the main root and lateral root of the plant are cut by 1 cm each.

[0059] (1) cultivate oak seedlings, the detailed steps are as follows:

[0060] Fresh oak seeds were harvested in November of that year, and after water flotation, the seeds were soaked in 20% hydrogen peroxide for disinfection for 1 hour, and the river sand was sterilized by high temperature and humidity: 121-126°C, 2 hours; the water content of the river sand was 18%; The Quercus japonica seeds were stored in sterilized and cooled river sand for 4 months, the temperature of the sand storage was 0-4°C, and the humidity was natural. Until March of the next year, sieve and select the seeds stored in sand, wash them with ultrapure water, soak the seeds with 0.2% acidic potassium permanganate solution for 1 hour, and then wash the seeds with sterile water until The color of the eluate is invisible to the na...

Embodiment 3

[0068] Example 3 (blank control) The effective inoculum amount of the bacterial agent of the present invention is 0 g, and the main root and lateral root of the plant are cut by 1 cm each.

[0069] (1) cultivate oak seedlings, the detailed steps are as follows:

[0070] Harvest fresh Quercus japonica seeds, soak them in 20% hydrogen peroxide and sterilize them for 1 hour after water flotation, and sterilize with high temperature and humidity in river sand: 121-126°C, 2 hours; the water content of river sand is 18%; Use sterilized and cooled river sand to store for 4 months, the temperature of the sand store is 0-4°C, and the humidity is natural. Until March of the next year, sieve and select the seeds stored in sand, wash them with ultrapure water, soak the seeds with 0.2% acidic potassium permanganate solution for 1 hour, and then wash the seeds with sterile water until The color of the eluate is invisible to the naked eye, and then soak the seeds in sterile water for 24 hou...

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Abstract

The invention belongs to the field of agricultural seedling raising, relates to a method for breeding mycorrhizal seedlings by truffles and quercus acutissima and particularly relates to a breeding method which can be used for obtaining truffle mycorrhizal seedlings through inoculating the quercus acutissima by effectively using truffle infection. The breeding method comprises the following steps: (1) preparing young quercus acutissima seedlings; (2) inoculating the truffles to the young quercus acutissima seedlings; and (3) carrying out adult seedling management. The method has the keys that the quercus acutissima is inoculated with the truffles, so that the development of roots of the quercus acutissima is facilitated, and the resistance and metabolic strength of the quercus acutissima can be improved; and after inoculation, the chlorophyll content, SOD enzyme activity and root activity of plants are all improved remarkably, the occurrence of roots is promoted, the ratio of an overground part to an underground part is increased, and the survival of the plants is facilitated. Through breeding the mycorrhizal seedlings by the method disclosed by the invention, a higher mycorrhiza infection rate can be obtained; and ingredients such as organic, water retention and inorganic ions are added into a microbial agent, so that the infection index of mycorrhizae is increased by using the characteristics such as hydrotropism and fertilizer tropism of plant seedlings.

Description

technical field [0001] The invention belongs to the field of agricultural seedling cultivation, and relates to a method for cultivating mycorrhizal seedlings with truffles and oak oaks, in particular to a method for cultivating truffle mycorrhizal seedlings by using truffles to infect and inoculate oak oaks. Background technique [0002] Truffle is a rare edible fungus. It is an ectomycorrhizal fungus formed by the symbiosis of ascomycete mycelia and forest roots. It is widely distributed in temperate regions of the earth, and most of Yunnan and Panxi, Sichuan are located in this range. Inside, truffle ascocarp is expensive, and it is a plant with high economic value. Since the edible ascocarp of truffles are born underground, it has brought great difficulties for humans to find and harvest truffles. In order to meet the needs of interests, there has been disorderly and excessive excavation. It can be seen that the realization of artificial cultivation of truffles is of grea...

Claims

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Application Information

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IPC IPC(8): A01G1/00A01G9/10
CPCA01G9/0299A01G22/00
Inventor 李小林叶雷张波吴晨光冯仁才周洁熊小龙吴翔谢丽源谭伟彭卫红黄忠乾甘炳成
Owner INST OF SOIL FERTILIZER SICHUAN ACAD OF AGRI SCI
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