Hyaluronic acid/polylactic acid-glycollic acid copolymer composite cartilage repair material with radial orientation pore structure and preparation method thereof

A glycolic acid copolymer and hyaluronic acid technology, used in medical science, prosthesis, tissue regeneration, etc., can solve the problems of cell infiltration and migration obstacles, restrict the exchange of substances, etc., to promote material exchange and biological signal communication, Improve secretion and deposition, achieve activation and maintenance effect

Active Publication Date: 2016-08-31
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the hydrogel can maintain the phenotype of chondrocytes, the dense network structure limits the exchange of substances
For the study of simply using materials to induce cartilage regeneration, the random macroporous structure also hinders the penetration and migration of cells into the scaffold to a certain extent.

Method used

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  • Hyaluronic acid/polylactic acid-glycollic acid copolymer composite cartilage repair material with radial orientation pore structure and preparation method thereof
  • Hyaluronic acid/polylactic acid-glycollic acid copolymer composite cartilage repair material with radial orientation pore structure and preparation method thereof
  • Hyaluronic acid/polylactic acid-glycollic acid copolymer composite cartilage repair material with radial orientation pore structure and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1) Completely dissolve 1 g of hyaluronic acid in 100 mL of deionized water, add 2 mL of methacrylic anhydride under stirring condition, keep the pH value of the solution at 8, and the temperature at 0 o Under the condition of C, the reaction was carried out for 12 hours, and the reactant was repeatedly precipitated by ethanol and dried to obtain methacrylic acid-modified hyaluronic acid;

[0028] 2) Dissolve the modified hyaluronic acid in deionized water, prepare a hyaluronic acid aqueous solution with a mass concentration of 5%, put the solution in a polyethylene mold, and set it at 37 o C for 1 hour at constant temperature;

[0029] 3) Put the polyethylene mold at -20 o C In the pre-cooled copper mold, the heat is transferred along the radial direction, and the solvent crystallization is also along the radial direction. After the solvent is completely crystallized, it is freeze-dried, and the dried scaffold is cross-linked under ultraviolet light for 2 hours to obt...

Embodiment 2

[0033] 1) Completely dissolve 2 g of hyaluronic acid in 100 mL of deionized water, add 4 mL of methacrylic anhydride under stirring conditions, and keep the pH value of the solution at 9 and the temperature at 0 o Under the condition of C, the reaction continued for 16 hours, and the reactant was repeatedly precipitated with ethanol and dried to obtain methacrylic acid-modified hyaluronic acid;

[0034] 2) Dissolve the modified hyaluronic acid in deionized water to prepare a hyaluronic acid aqueous solution with a mass concentration of 8%, put the solution in a polyethylene mold, and set it at 37 o C under constant temperature for 2 hours;

[0035] 3) Put the polyethylene mold at -15 oC In the pre-cooled copper mold, the heat is transferred along the radial direction, and the solvent crystallization is also along the radial direction. After the solvent is completely crystallized, it is freeze-dried, and the dried scaffold is cross-linked under ultraviolet light for 4 hours t...

Embodiment 3

[0039] 1) Completely dissolve 3 g of hyaluronic acid in 100 mL of deionized water, add 6 mL of methacrylic anhydride under stirring conditions, and keep the pH value of the solution at 8 and the temperature at 0 o Under the condition of C, the reaction continued for 24 hours, and the reactants were repeatedly precipitated with ethanol and dried to obtain methacrylic acid-modified hyaluronic acid;

[0040] 2) Dissolve the modified hyaluronic acid in deionized water to prepare a hyaluronic acid aqueous solution with a mass concentration of 8%, put the solution in a polyethylene mold, and set it at 37 o C under constant temperature for 1.5 hours;

[0041] 3) Put the polyethylene mold at -20 o C In the pre-cooled copper mold, the heat is transferred along the radial direction, and the solvent crystallization is also along the radial direction. After the solvent is completely crystallized, it is freeze-dried, and the dried scaffold is cross-linked under ultraviolet light for 4 ho...

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Abstract

The invention discloses a hyaluronic acid / polylactic acid-glycollic acid copolymer composite cartilage repair material with a radial orientation pore structure and a preparation method thereof. The hyaluronic acid / polylactic acid-glycollic acid copolymer composite cartilage repair material has a tubular pore structure distributed along radial orientation in a columnar composite stent, wherein the composite bracket is composed of hyaluronic acid and a polylactic acid-glycollic acid copolymer; the hyaluronic acid has the capability of inducing cartilage regeneration and can promote the differentiation of stem cells to cartilage cells and improve the secretion and deposition of the extracellular matrix of cartilage; the polylactic acid-glycollic acid copolymer provides mechanical support. The pore structure of radial orientation can promote surrounding tissue cells to transfer into the stent and promote material exchange and biological signal communication between regenerated tissues and primary tissues so as to increase the tissue regeneration speed. The cartilage repair material can effectively induce in-situ regeneration of the cartilage tissue and can be used for repairing full-layer cartilage defect.

Description

technical field [0001] The invention relates to a hyaluronic acid / polylactic acid-glycolic acid copolymer composite cartilage repair material with a radially oriented hole structure and a preparation method thereof. Background technique [0002] Articular cartilage is a dense connective tissue that plays an extremely important role in buffering the stress between joints and reducing friction between articular surfaces. However, articular cartilage is a kind of tissue that is easily damaged. Factors such as obesity, arthritis, and sports trauma may cause damage to the joints. Even a small area of ​​defect has the potential risk of serious degeneration. Due to the dense structure of articular cartilage and the low proliferation ability of chondrocytes, it is difficult for articular cartilage to repair itself once it is damaged. Therefore, the regeneration of its structure and function has always been a clinical challenge that is difficult to completely overcome. There are ma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/56A61L27/26A61L27/50
CPCA61L27/26A61L27/50A61L27/56A61L2430/02C08L5/08C08L67/04
Inventor 高长有代元坤
Owner ZHEJIANG UNIV
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