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A detection method for simultaneously determining the contents of flavonoids and terpene lactones in Shuxuening

A technology of flavonoid glycosides and ginkgolides, which is applied in the field of high-performance liquid chromatography detection, can solve the problems of insufficient process simplicity and complicated operation steps, and achieve the effects of low cost, good durability and easy realization

Active Publication Date: 2017-12-29
HEBEI SHINEWAY PHARMA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above method uses different detection methods to detect terpene lactones and flavonol glycosides respectively. The disadvantage is that different instruments and different sample processing conditions are required to inject samples separately, resulting in complicated operation steps and insufficient process simplicity.

Method used

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  • A detection method for simultaneously determining the contents of flavonoids and terpene lactones in Shuxuening
  • A detection method for simultaneously determining the contents of flavonoids and terpene lactones in Shuxuening
  • A detection method for simultaneously determining the contents of flavonoids and terpene lactones in Shuxuening

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] (1) Chromatographic conditions: the stationary phase is a chromatographic column filled with octadecyl bonded silica gel; the UV detector is connected in series with the ELSD detector; the UV detection wavelength is 360nm; the flow rate is 1mL / min; the mobile phase A is 0.05% tetrahydrofuran -0.1% formic acid-5% methanol-water mixed solvent, the volume ratio is 1:2:10:950; mobile phase B is a mixed solvent of 0.1% tetrahydrofuran-methanol-acetonitrile solution, the volume ratio is 1:1000, wherein Methanol-acetonitrile solution is a solution with a volume ratio of methanol and acetonitrile of 1:1; gradient elution is performed, and the gradient conditions are:

[0041]

[0042]

[0043] ; ELSD conditions are: drift tube temperature 110°C, carrier gas flow rate 2.5L / min, gain value 1.

[0044] (2) Reference substance stock solution: accurately weigh rutin, isoquercitrin, narcissin, 3-O-{2-O-[6-O-(p-hydroxy-trans-coumaroyl)-glucosyl] -(1-2)rhamnosyl}kaempferol, quer...

Embodiment 2

[0059] (1) Chromatographic conditions: the stationary phase is a chromatographic column filled with octadecyl bonded silica gel; the UV detector is connected in series with the ELSD detector; the UV detection wavelength is 360nm; the flow rate is 1mL / min; the mobile phase A is 0.05% tetrahydrofuran -0.1% formic acid-5% methanol-water mixed solvent, the volume ratio is 1:2:10:950; mobile phase B is a mixed solvent of 0.1% tetrahydrofuran-methanol-acetonitrile solution, the volume ratio is 1:1000, wherein Methanol-acetonitrile solution is a solution with a volume ratio of methanol and acetonitrile of 1:1; gradient elution is performed, and the gradient conditions are:

[0060] time (min)

A(％)

B(％)

0～30

90→80

10→20

30～33

80→79

20→21

33～47

79→79

21→21

47～63

79→67

21→33

63～74

67→45

33→55

74～75

45→90

55→10

[0061] ; ELSD conditions are: drift tube temperature 110°C, carrier gas flow rate 2.5...

Embodiment 3

[0077] (1) Chromatographic conditions: the stationary phase is a chromatographic column filled with octadecyl bonded silica gel; the UV detector is connected in series with the ELSD detector; the UV detection wavelength is 360nm; the flow rate is 1mL / min; the mobile phase A is 0.05% tetrahydrofuran -0.1% formic acid-5% methanol-water mixed solvent, the volume ratio is 1:2:10:950; mobile phase B is a mixed solvent of 0.1% tetrahydrofuran-methanol-acetonitrile solution, the volume ratio is 1:1000, wherein Methanol-acetonitrile solution is a solution with a volume ratio of methanol and acetonitrile of 1:1; gradient elution is performed, and the gradient conditions are:

[0078] time (min)

A(％)

B(％)

0～30

90→80

10→20

30～33

80→79

20→21

33～47

79→79

21→21

47～74

79→45

21→55

74～75

45→90

55→10

[0079] ; ELSD conditions are: drift tube temperature 110°C, carrier gas flow rate 2.5L / min, gain value 1.

[0080]...

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Abstract

The invention relates to the field of medicine detection, in particular to a high-performance liquid chromatography detecting method for measuring the content of flavonoid compounds and terpene lactone compounds in ginkgo leaves (or ginkgo leaf extract) or preparations of the ginkgo leaves at the same time. The HPLC-DAD-ELSD method is set up, the method can measure the eight flavonoid compounds and three terpene lactone compounds in the ginkgo leaves (or the ginkgo leaf extract) or preparations of the ginkgo leaves at the same time, the measurement result is analyzed, and a new analysis method and a basis are provided for quality control over the ginkgo leaf extract and the preparations of the ginkgo leaf extract.

Description

technical field [0001] The invention relates to the field of drug detection, more specifically, the invention relates to a high-performance liquid chromatography detection method for simultaneously determining the contents of flavonoids and terpene lactones in Shuxuening injection. Background technique [0002] Shuxuening Injection is clinically used for the treatment of cardiovascular diseases and peripheral circulatory disorders, and works through multiple targets and multiple links. It can reduce the cardiovascular risk caused by high homocysteine ​​(Hcy); reduce the pro-inflammatory factor plasma C-reactive protein (CRP) related to the occurrence, evolution and development of atherosclerosis; reduce the strong vasoconstriction and Thromboxane B2 (TXB2) that promotes platelet aggregation; reduces serum lipid peroxide (LOP), which can cause various damages to the structure and function of cells and cell membranes; reduces endogenous vasoconstrictor activity widely present ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/78
CPCG01N30/02G01N30/78G01N2030/027
Inventor 常艳旭高秀梅田吉李晋巴晓雨张纲李志刚关秀伟
Owner HEBEI SHINEWAY PHARMA
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