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Method for preparing lipopeptide biological surfactant

A technology of biological surface and active agent, applied in the biological field, can solve the problems of complicated separation and purification process, long fermentation period and high cost of raw materials, and achieve the effects of cheap and easy-to-obtain raw materials, short fermentation period and lower production cost.

Inactive Publication Date: 2016-08-10
QINGDAO UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0005] Aiming at technical problems such as high cost of raw materials, long fermentation cycle and complicated separation and purification process in the prior art, the purpose of the present invention is to provide a method for producing lipopeptide biosurfactant by fermentation of industrial waste, which has the advantages of process Simple, short fermentation cycle, less environmental pollution, low cost, suitable for industrial production

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  • Method for preparing lipopeptide biological surfactant
  • Method for preparing lipopeptide biological surfactant
  • Method for preparing lipopeptide biological surfactant

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Effect test

Embodiment 1

[0027] Strain screening

[0028] The salt-tolerant Brevibacterium Y-1-4 of the present invention is screened using the following method, and the specific steps are as follows:

[0029] (1) Spot the 317 strains of bacteria that have been isolated and purified from the soil on the blood plate and cultivate them at 30-37°C for 12-24 hours.

[0030] (2) Select the strain with the larger hemolytic circle in step (1) for the oil discharge circle test.

[0031] (3) The blood plate in step (1) was purchased from Beijing Luqiao Technology Co., Ltd.

[0032] (4) The specific steps of the oil drain ring experiment used in step (2): Take a petri dish with a diameter of 9 cm, add 20 mL of distilled water, and drop 200 μL of liquid paraffin into the center of the water to slowly form a thin oil film. Slowly add 10μL of supernatant to the liquid paraffin and measure the diameter of the oil drain ring. The strain with the largest oil discharge ring (7.5cm) is Brevibacterium halotolerans Y-1-4.

[0033...

Embodiment 2

[0038] Utilization of Carbon Source by Brevibacterium halotolerans Y-1-4

[0039] Brevibacterium halotolerant Y-1-4 can use various carbon sources to produce lipopeptide biosurfactants, mainly glucose, lactose, sucrose, soluble starch, maltose, soybean molasses, glycerin, sucrose molasses. Other components are: yeast powder 3g / L, K 2 HPO 4 3g / L, NaH 2 PO 4 3g / L, MgSO 4 0.1g / L, FeSO 4 0.05g / L, NaCl 5g / . The carbon source concentration is 2%L, the sample volume is 50mL / 250mL Erlenmeyer flask, the inoculation volume is 4%, the culture temperature is 30°C, and the shaker speed is 160rpm. The results are shown in Table 1 below.

[0040] Table 1 shows the crude weight of biosurfactants corresponding to different carbon sources

[0041]

Embodiment 3

[0043] Utilization of Nitrogen Source by Brevibacterium halotolerans Y-1-4

[0044] Brevibacterium halotolerant Y-1-4 can use various nitrogen sources to produce lipopeptide biosurfactants, mainly including soybean meal, peptone, beef powder, corn steep liquor, yeast powder, ammonium nitrate, ammonium sulfate, and urea. Other components are: sucrose molasses 20g / L, K 2 HPO 4 3g / L, NaH 2 PO 4 3g / L, MgSO 4 0.05g / L, FeSO 4 0.1g / L, NaCl 4g / L. The nitrogen source concentration is 3g / L, the sample volume is 50mL / 250mL Erlenmeyer flask, the inoculation volume is 5%, the culture temperature is 28°C, and the shaker rotation speed is 140rpm. The results are shown in Table 2 below.

[0045] Table 2 shows the crude weight of biosurfactants corresponding to different nitrogen sources

[0046]

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Abstract

The invention discloses a method for preparing a lipopeptide biological surfactant. The adopted bacterial strain is brevibacterium halotolerans Y-1-4, the collection number is CGMCC No.8823, cheap saccharose molasses serves as a carbon source of a medium, corn steep liquor serves as a nitrogen source, nutrient elements such as other phosphorus sources and inorganic salt are added, the mixture is fermented to be subjected to the acid precipitation step, the organic solvent extraction step and the like, and the lipopeptide biological surfactant is obtained. The critical micelle concentration of the lipopeptide biological surfactant is 21.512 mg / L, and the surface tension value of distilled water can be reduced to 28 mN / m or below from 72 mN / m (25 DEG C); the raw materials required by the lipopeptide biological surfactant produced with the method are cheap and easy to obtain. According to the method, the kinds of producing bacteria of the lipopeptide biological surfactant are enriched, the production cost of lipopeptide is reduced through the cheap raw materials, and it is possible that the biological surfactant is more widely applied.

Description

Technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to a preparation method of a lipopeptide biosurfactant. Background technique [0002] Lipopeptide biosurfactants are a class of environmentally friendly surface active compounds produced by microorganisms. Compared with chemically synthesized surfactants, in addition to common characteristics, they are non-toxic or low-toxic and have good environmental compatibility. , Biodegradable, will not cause re-pollution and many other advantages, can use cheap agricultural and sideline products for fermentation production, used for biological environmental treatment, has stronger surface activity and emulsification ability, and still maintains under extreme temperature, pH and salt concentration High stability, some also have antibacterial, antiviral, antitumor and other physiological activities. Lipopeptide biosurfactants have important application prospects in petrochemical, environment...

Claims

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Application Information

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IPC IPC(8): C12P21/00C12R1/13
CPCC12P21/00
Inventor 李红芳李俊峰滕丽丽段效辉梁生康
Owner QINGDAO UNIV OF SCI & TECH
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