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Method for producing biodiesel by means of lignocellulose material

A technology of lignocellulose and biodiesel, which is applied in the direction of biofuel, fat production, fatty acid production, etc., can solve the problems of low oil output, high production cost, and difficult resource utilization of by-products, so as to increase production and reduce reagent costs , Improve the effect of C/N ratio

Active Publication Date: 2016-06-22
江西碳金科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of the above problems, the object of the present invention is to provide a method for producing biodiesel from lignocellulosic raw materials, aiming to solve the outstanding problems of high production cost, low oil yield and difficult resource utilization of by-products in existing methods

Method used

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  • Method for producing biodiesel by means of lignocellulose material

Examples

Experimental program
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Effect test

Embodiment 1

[0043] 1) Pretreatment of corn stalks and preparation of hydrolyzate: Weigh 50 g of corn stalks passed through a 40-mesh sieve, add 200 g of KOH-glycerin-methanol solution (KOH 2 g, glycerin 4 g, methanol 194 g), solid-liquid ratio 25% (w / w) , treated in a pretreatment tank at 180°C for 15 minutes; in-situ evaporation and condensation to recover methanol, adding water to adjust the solid-liquid ratio to 10% (w / w), adding H 2 SO 4 Adjust the pH to 4.8, add cellulase 15FPU / g, β-glucosidase 30CBU / g and xylanase 100U / g, hydrolyze in a water-bath shaker at 50°C and 200rpm for 48h to obtain a hydrolyzate; boil the hydrolyzate for 10min , vacuum filtration to obtain the hydrolyzed supernatant, in which the concentrations of glucose, xylose and glycerol were 30.1g / L, 18.5g / L and 15.8g / L respectively, and the C / N ratio was 72. After adjusting the pH to 6.0, it was extinguished at 121°C. After 20 minutes of bacteria, it is ready for use;

[0044] 2) Insert Cryptococcus curvatus ATCC20...

Embodiment 2

[0049] 1) Rice straw pretreatment and preparation of hydrolyzate: Weigh 20g of rice straw passed through a 20-mesh sieve, add 40g of KOH-glycerin-methanol solution (0.2g of KOH, 2g of glycerol, 37.8g of methanol), and the solid-to-liquid ratio is 50% (w / w ), treated in a pretreatment tank at 80°C for 2 hours; in situ evaporation to remove methanol, adding water to adjust the solid-liquid ratio to 5% (w / w), adding H 2 SO 4 Adjust the pH to 4.5, add cellulase 20FPU / g, β-glucosidase 40CBU / g and xylanase 100U / g, hydrolyze in a water-bath shaker at 50°C and 200rpm for 72h to obtain a hydrolyzate; boil the hydrolyzate for 10min , vacuum filtration to obtain the hydrolyzed supernatant, in which the concentrations of glucose, xylose and glycerin were 14.5g / L, 8.4g / L and 4.8g / L respectively, and the C / N ratio was 62. After adjusting the pH to 5.5, it was extinguished at 121°C Sterilize for 15 minutes and set aside;

[0050] 2) Insert Rhodosporidium toruloides AS2.1389 (purchased from...

Embodiment 3

[0055] 1) Pretreatment of cotton stalks and preparation of hydrolyzate: Weigh 10 g of cotton stalks passed through a 40-mesh sieve, add 100 g of NaOH-glycerin-methanol solution (2 g of NaOH, 2.5 g of glycerol, and 90 g of methanol), and the solid-to-liquid ratio is 10% (w / w ), treated in a pretreatment tank at 140°C for 2 hours; in situ evaporation to remove methanol, adding water to adjust the solid-liquid ratio to 25% (w / w), adding H 3 PO 4 Adjust the pH to 5.5, add cellulase 15FPU / g, β-glucosidase 30CBU / g and xylanase 100U / g, hydrolyze in a water-bath shaker at 50°C and 200rpm for 72h to obtain a hydrolyzate; boil the hydrolyzate for 10min , vacuum filtration to obtain the hydrolyzed supernatant, in which the concentrations of glucose, xylose and glycerin were 81.1g / L, 54.5g / L and 60.8g / L respectively, and the C / N ratio was 102. After adjusting the pH to 6.0, it was extinguished at 121°C. Sterilize for 15 minutes and set aside;

[0056] 2) Lipomycessstarkeyi AS2.1560 (pur...

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Abstract

The invention is suitable for the field of biomass energy, and provides a method for producing biodiesel by means of a lignocellulose material. The method comprises the steps that pretreatment is conducted on the lignocellulose material through an alkali-glycerin-methanol mixed solution produced in the process that the biodiesel is prepared by conducting single-step base catalysis transesterification on microbial lipid; after in-situ evaporation is conducted on the raw material, and methanol is subjected to condensation recovery, water is added to reach the proper solid-to-liquid ratio, after acid neutralization is conducted, an appropriate number of enzymes are added for hydrolysis, solid-liquid separation is conducted, and hydrolysate is obtained; oleaginous yeast is introduced and synthesizes lipid by means of carbohydrates and glycerin in the hydrolysate; microbial thalli containing oil are collected, intracellular lipid is extracted, the biodiesel is prepared through single-step base catalysis transesterification, n-hexane is added for extraction, the biodiesel is obtained on the upper layer, the alkali-glycerin-methanol solution is obtained on the lower layer, and the alkali-glycerin-methanol solution is recycled for pretreating lignocellulose. According to the method for producing the biodiesel by means of the lignocellulose material, strong alkali, by-product glycerin and methanol are used for pretreating the lignocellulose; meanwhile, the glycerin further serves as a carbon source of oleaginous microorganisms to be subjected to in-situ integration recycling, the cost is greatly reduced, and the significant economic benefit is achieved.

Description

technical field [0001] The invention belongs to the technical field of biorefining and bioenergy, and in particular relates to a method for producing biodiesel from lignocellulosic raw materials. Background technique [0002] Under certain conditions in nature, some microorganisms can use carbohydrates, hydrocarbons, carbon dioxide, etc. as carbon sources to synthesize and store a large amount of oil in the body, and any oil that can accumulate in the cell exceeds 20% of the dry weight of the cell (w / w, where w / w represents the mass ratio, the same below) are called oil-producing microorganisms. Some oleaginous microorganisms accumulate oil in their cells that exceeds 70% of their dry cell weight. Microbial oil, especially the oil produced by oleaginous fungi, its main component is triglyceride, and its fatty acid composition is similar to that of commercial animal and vegetable oils. 14 -C 22 Mainly long chain fatty acids. Compared with animal and vegetable oils, micro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C11C3/04C11C1/08C11B1/02C10L1/02
CPCC10L1/02C10L2290/543C11B1/025C11C1/08C11C3/04C12P7/649Y02E50/10
Inventor 龚志伟赵觅
Owner 江西碳金科技有限公司
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