Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of gluco-oligosaccharide and its preparation method and application

A technology of oligoglucosaccharide and glucose, which is applied in the field of agricultural application, can solve the problems of long enzymatic hydrolysis cycle, low efficiency and high production cost, and achieves the effects of low cost, simple preparation process, and easy separation and purification

Active Publication Date: 2018-01-05
NANJING SOUTHERN ELEMENT BIOTECHNOLOGY CO LTD
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing enzymatic hydrolysis method is limited by the source and type of enzyme, and generally has the disadvantages of long enzymatic hydrolysis cycle, low efficiency and high production cost.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of gluco-oligosaccharide and its preparation method and application
  • A kind of gluco-oligosaccharide and its preparation method and application
  • A kind of gluco-oligosaccharide and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] This example illustrates the method of mixing Agrobacterium ZX09 and Paenibacillus S09 through mixed culture and fermentation to obtain oligoglucose.

[0033] a. Preparation of seed solution: Inoculate a single colony of ZX09 bacteria into an inorganic salt-sucrose liquid medium, and culture it with shaking at 30°C for 24 hours. The medium composition is: 20g sucrose, 0.5g sodium nitrate, 0.5g potassium dihydrogen phosphate, Anhydrous calcium chloride 0.1g, heptahydrate magnesium sulfate 0.3g, ferrous sulfate heptahydrate 0.02g, zinc chloride 0.003g, manganese sulfate 0.001g, water 1000mL, pH 7.0. Autoclave at 121°C for 20min. A single colony of S09 bacteria was inoculated into LBO liquid medium and cultured with shaking at 35°C for 24 hours. The medium composition was 0.5g sodium chloride, 0.5g yeast extract, 1.0g peptone, 0.5g oatmeal, and autoclaved at 121°C. 20min.

[0034] b. Mixed culture: inoculate the cultured ZX09 bacteria and S09 bacteria seed liquid into the mixe...

Embodiment 2

[0038] Preparation of oligoglucose derivatives.

[0039] The purified oligoglucose was prepared into a 10mg / mL aqueous solution, and NaOH with a final concentration of 0.2M was added to react for 2-5 hours. After neutralization with HCl, it was desalted by Sephadex G25, and the elution peak sample of oligoglucose was collected. Oligosaccharide derivative II with succinyl modification group removed. The purified oligoglucose was prepared into a 10mg / mL aqueous solution, added with a final concentration of 0.5M HCl, and reacted for 5h. After neutralization with NaOH, it was desalted by Sephadex G25. The oligoglucose elution peak sample was collected and removed at the same time. Succinyl and pyruvate modified oligosaccharide derivatives III.

Embodiment 3

[0041] The control of gluco-oligosaccharides to Arabidopsis thaliana infection by Botrytis cinerea.

[0042] Prepare 0.2-1.0g / L oligoglucose aqueous solution and spray it evenly on the leaves of Arabidopsis plants that grow to a suitable size, spray once a day, and spray with water as a control. Two days after the pretreatment of oligoglucose, the cultured Botrytis cinerea was punched on the solid medium, and the mold agar block was attached to the Arabidopsis leaves for infection culture. 1-3 days later, observe the infection degree of the leaves of the control group and the oligoglucose group.

[0043] The results showed that spraying oligoglucose solution in advance can reduce the infection rate of Arabidopsis plant leaves, and the disease plaques were significantly reduced. image 3 Shown are the leaves of Arabidopsis plants after spraying clean water and spraying the oligoglucose solution with a concentration of 0.5 g / L in advance.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
Login to View More

Abstract

The invention discloses glucooligosaccharide with succinic acid and pyruvoyl modified groups and a de-modified derivative of the glucooligosaccharide. A main chain of the glucooligosaccharide is composed of eight glycosyls and is free of branched chain connection, and the glycosyls of the main chain are connected by one alpha-1, 3-glycosidic bond and six beta-1, 3 glycosidic bonds. The glucooligosaccharide is obtained by directly separating from fermentation supernate after passing liquid mixed fermentation Agrobacterium ZX09 and Paenibacillus S09, and a glucooligosaccharide dry powder product is obtained after subjecting the fermentation supernate to alcohol precipitation, centrifuging and drying. The preparation method is quick, effective, simple in process, high in yield, low in cost, free of pollution and suitable for industrialized production. The glucooligosaccharide prepared by the method is high in water solubility, uniform in molecular weight and structure, easy to prepare and purify, high in bioactivity and capable of effectively activating resistant, to pathogenic fungi, of plants, can serve as a plant induced resistance agent and a plant protective agent, has resistance inducing effect on crops remarkably better than that of kelp oligosaccharide and has wide application value in the aspects of prevention and control of plant diseases and pests.

Description

Technical field [0001] The invention belongs to the technical field of agricultural applications, and specifically relates to an oligoglucose and its derivatives prepared by microbial mixed fermentation, as well as a preparation method of oligoglucose and its application in plant resistance and plant protection. Background technique [0002] Oligosaccharides, also known as oligosaccharides or oligosaccharides, are generally straight or branched carbohydrate substances formed by connecting 3 to 10 monosaccharides through glycosidic bonds. According to whether it can be metabolized and absorbed by the human body and whether it has biological functions, oligosaccharides can be divided into ordinary oligosaccharides and functional oligosaccharides. Ordinary oligosaccharides such as sucrose, maltose, lactose and trehalose can be digested and absorbed by the human body. They are energy substances and have no special physiological functions. Functional oligosaccharides generally have l...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/02C12P39/00C12P19/08A01N43/90A01N43/16A01P21/00
CPCA01N43/16A01N43/90C08B37/0024C12P19/08C12P39/00
Inventor 张建法程瑞李晶
Owner NANJING SOUTHERN ELEMENT BIOTECHNOLOGY CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com