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Method for increasing leaching rate of polyphenols in dendrobium officinale kimura et migo

A Dendrobium officinale and extraction rate technology, which is applied in the field of traditional Chinese medicine preparation, can solve the problems of high energy consumption, large solvent consumption, and low polyphenol extraction rate, and achieve the effects of low energy consumption, low equipment investment, and enhanced health care functions

Active Publication Date: 2016-05-11
YUNNAN MINZU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These preparation methods are mostly conventional methods, with low polyphenol extraction rate, large solvent consumption, large energy consumption, and time-consuming

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0032] (1) Preparation of standard curve: Take an appropriate amount of gallic acid, weigh it accurately, add water to make a gallic acid standard stock solution with a concentration of 0.050 mg / mL. Accurately measure gallic acid standard stock solution 0.2, 0.4, 0.6, 0.8, 1.0 into 25mL stoppered test tubes, add water to make up to 1.0mL, add 0.3mL of folinol reagent, mix well, then add 1.5mL of 7% carbonic acid Sodium solution, add water to make up to 10mL, shake well, and let stand at room temperature for 20min. Using the reagent blank as a reference, measure the absorbance at a wavelength of 760 nm with a UV-Vis spectrophotometer. Draw a standard curve with absorbance as the ordinate and concentration as the abscissa.

[0033] (2) Preparation and determination of sample solution: Take 0.5 g of sample powder (passed through a No. 3 sieve), add 75% ethanol, weigh, extract for 2 hours, weigh and make up for weight loss, and use it as a sample solution. Draw 1.00mL of the sam...

Embodiment 1

[0043] A, fresh dendrobium candidum strips are cleaned and dried;

[0044] B. The fresh strips of Dendrobium candidum of step A are placed in a drying oven, dried at 97°C for 3.25 hours, so that the moisture content is 20%, and cut into short strips of 3.5 cm;

[0045] C, the dendrobium candidum short strips of step B are obtained by microwave puffing and breaking the wall; the process condition of the microwave puffing and breaking is 700 watts of power, and the puffing and breaking time is 33 seconds, so that the puffed and broken product is obtained;

[0046] D. After cooling the puffed and broken wall product in step C for 15 minutes, it is crushed (passed through a 200-mesh sieve) and packed.

[0047]E. Add 60 ml of 75% ethanol solvent to the Dendrobium officinale powder (10 grams) obtained in step D and extract at 74°C for 4 times, each time for 2 hours, combine the extracts, filter and concentrate to obtain an extract with a relative density of 1.2 ;

[0048] F. After...

Embodiment 2

[0051] A, fresh dendrobium candidum strips are cleaned and dried;

[0052] B, the fresh strip of dendrobium candidum of step A is placed in drying box, is dried at 95 ℃ for 3.5 hours, makes its moisture content be 30%, is cut into the short strip of 4.0 centimeters;

[0053] C, the dendrobium candidum short strips of step B are obtained by microwave puffing and breaking the wall; the process conditions of the microwave puffing and breaking are power 650w, and the puffing and breaking time is 35 seconds to obtain the puffed and broken product;

[0054] D. After cooling the puffed broken wall product in step C for 17 minutes, it is crushed (passed through a 200-mesh sieve) and packed.

[0055] E. Add 80 ml of 80% methanol solvent to the Dendrobium officinale powder (10 grams) obtained in step D and extract at 60°C for 4 times, each time for 3 hours, combine the extracts, filter and concentrate to obtain an extract with a relative density of 1.3 ;

[0056] F. After drying the e...

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Abstract

The invention discloses a method for increasing the leaching rate of polyphenols in dendrobium officinale kimura et migo. The method comprises the steps of pre-treatment, extraction and post-treatment, and specifically comprises the following steps of cleaning and air-drying raw materials fresh dendrobium officinale kimura et migo twigs, drying the dendrobium officinale kimura et migo twigs for 3.0 to 3.5h at 95 to 100 DEG C until the water content is 15 to 30 percent, and cutting the dendrobium officinale kimura et migo twigs into 3.0 to 4.0cm short twigs a; performing expansion wall breaking and crushing on the short twigs a, sieving the crushed short twigs a to obtain dendrobium officinale kimura et migo extract prepared material b by virtue of a 100 to 300-mesh sieve, adding an alcohol solvent in an amount which is 4 to 8 times the weight of the dendrobium officinale kimura et migo extract prepared material b, performing extraction 3 to 4 times at 60 to 80 DEG C for 1 to 3h every time, mixing extracting solutions, and performing filtration and concentration to obtain extract c with relative density of 1.1 to 1.3; drying, crushing and sieving the extract c to obtain a target extract. The method is less in production equipment investment, a process is easy to operate, conditions are easily reached, a preparation process is low in energy consumption and cost, convenience in processing is achieved, and adopted reagents are nontoxic, harmless, safe and environment-friendly; the polyphenols in a prepared dendrobium officinale kimura et migo product can be easily leached, and the leaching rate of the polyphenols in the dendrobium officinale kimura et migo is increased.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine preparation, and in particular relates to a method for increasing the leaching rate of polyphenols in Dendrobium officinale. Background technique [0002] Dendrobium officinale ( Dendrobium officinale Kimura et Migo) is a plant of the genus Dendrobium in the family Orchidaceae, which has the effects of strengthening the spleen and nourishing the stomach, nourishing yin and kidney, nourishing the lungs and promoting body fluid, etc. It is used for the treatment of chronic atrophic gastritis, hypertension, diabetes, anti-tumor, anti-aging, etc. Dendrobium officinale has various chemical components, including polysaccharides, polyphenols, alkaloids, glycosides and amino acids, among which polyphenols are one of the important physiologically active components of Dendrobium officinale, mainly including phenanthrenes, stilbenes, bibenzyls and lignans class of compounds. Bibenzyl c...

Claims

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Application Information

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IPC IPC(8): A61K36/8984
CPCA61K36/8984A61K2236/15A61K2236/17A61K2236/333A61K2236/39A61K2236/51
Inventor 黄相中彭金辉郭俊明李婷婷
Owner YUNNAN MINZU UNIV
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