Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Listerella selective culture medium

A technology for selecting the culture medium and Listeria, which is applied in the field of microbial culture inspection, can solve the problems of discarding, delaying diagnosis and treatment time, and low positive rate of Listeria culture, achieving reliable detection and high isolation rate

Inactive Publication Date: 2016-05-04
刘名霞
View PDF5 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the positive rate of Listeria culture is low, only about 36%, mainly because the bacteria are easily considered as contaminated bacteria and discarded during the clinical culture process, and Staphylococcus aureus, Escherichia coli, pneumococcus, etc. Grows faster than Listeria on current media and quickly overwhelms the media, making Listeria unable to grow and delaying diagnosis and treatment

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1, the formula for preparing 1000ml medium contains: yeast extract powder 5g; beef powder 10g; casein peptone 6g; agar 15g; glucose 4g; sodium chloride 5g; sodium dihydrogen phosphate 1g; g; yeast zinc 0.01g; N-acetyl-D-glucosamine 0.01g; bacitracin 0.003g; sterile equine hemoglobin 20g, add distilled water to 1000ml. Preparation method: Weigh yeast extract powder; beef powder; casein peptone; agar; glucose; sodium chloride; sodium dihydrogen phosphate; Cool to 45°C, add sterilized N-acetyl-D-glucosamine; sterilized bacitracin; sterile equine blood albumin, mix evenly, dilute to 1000ml with sterilized distilled water, and dispense.

Embodiment 2

[0025] Example 2, the formula for preparing 1000ml medium contains: yeast extract powder 5g; beef powder 6g; casein peptone 8g; agar 15g; glucose 3g; sodium chloride 5g; sodium dihydrogen phosphate 3g; g; yeast zinc 0.05g; N-acetyl-D-glucosamine 0.03g; bacitracin 0.001g; optical glutathione 0.1g; sterile equine hemoglobin 40g, add distilled water to 1000ml. Preparation method: Weigh yeast extract powder; beef powder; casein peptone; agar; glucose; sodium chloride; sodium dihydrogen phosphate; Cool to 45°C, add sterilized optical glutathione; sterilized N-acetyl-D-glucosamine; sterilized bacitracin; sterile horse blood protein, mix evenly, dilute to 1000ml with sterilized distilled water, and dispense.

Embodiment 3

[0026] Example 3, the formula for preparing 1000ml medium contains: yeast extract powder 5g; beef powder 8g; casein peptone 6g; agar 18g; glucose 5g; sodium chloride 5.5g; sodium dihydrogen phosphate 2g; 0.5g; yeast zinc 0.03g; N-acetyl-D-glucosamine 0.05g; bacitracin 0.001g; Preparation method: Weigh yeast extract powder; beef powder; casein peptone; agar; glucose; sodium chloride; sodium dihydrogen phosphate; γ-hydroxyarginine; yeast zinc; Sterilize for 15 minutes, cool to 45°C, add sterilized N-acetyl-D-glucosamine; sterilized bacitracin; sterile horse blood albumin, mix evenly, distill the volume to 1000ml with sterilized distilled water, and then pack.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a Listerella selective culture medium, belonging to the field of inspection microbe growth. The invention is characterized in that every 1000ml of culture medium contains yeast leaching powder, beef powder, casein peptone, agar, glucose, sodium chloride, yeast zinc, sodium dihydrogen phosphate, gamma-hydroxy arginine, N-acetyl-D-glucose amine, glutathione, bacitracin, sterile horse blood protein and the balance of distilled water. Compared with the prior art, the culture medium disclosed by the invention has the characteristics of high detection reliability and high separation rate of Listerella.

Description

technical field [0001] The invention relates to the field of test microorganism culture, in particular to a listeria selection medium. Background technique [0002] Microbial culture is a technology that uses artificial methods to grow and reproduce microorganisms, and selective medium is a medium designed to promote or inhibit certain types of organisms (such as cells or bacteria, etc.), using this medium can Separation of desired microorganisms from confounding microorganisms. Listeria monocytogenes is a Gram-positive bacillus, a facultative anaerobic bacterium widely distributed in the natural environment, such as soil, sewage, feed, animals, healthy people and various raw and ready-to-eat foods, which can cause Listeriosis in humans, Listeria is an intracellular parasite, most healthy people infected with listeriosis are asymptomatic or have self-limited flu-like symptoms and gastrointestinal symptoms, such as headache, fever, chills, Muscle pain, abdominal pain, diarr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04C12R1/01
CPCC12Q1/045
Inventor 刘名霞
Owner 刘名霞
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com