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A phycocyanin-polylactic acid-doxorubicin micelle for inhibiting brain cancer and its preparation method and application

A technology of phycocyanin and doxorubicin, which is applied in the field of medical materials, can solve problems such as the inability to overcome the blood-brain barrier, and achieve the effect of improving stability and reducing cytotoxicity

Active Publication Date: 2018-12-14
SOUTH CHINA NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, none of these drugs can overcome the problem of the blood-brain barrier and be used in the treatment of brain cancer

Method used

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  • A phycocyanin-polylactic acid-doxorubicin micelle for inhibiting brain cancer and its preparation method and application
  • A phycocyanin-polylactic acid-doxorubicin micelle for inhibiting brain cancer and its preparation method and application
  • A phycocyanin-polylactic acid-doxorubicin micelle for inhibiting brain cancer and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Example 1 Self-assembly synthesis of phycocyanin-polylactic acid micelles (PC-PLEL micelles)

[0062] 1. Preparation method

[0063] (1) Accurately weigh 0.2g of phycocyanin powder, dissolve it into a 5 mg / mL phycocyanin solution with 10ml PBS (pH=7.4), stir for 30min, add 0.184g polylactic acid copolymer dissolved in 7.36ml DMSO ( pla3600-peg2000-pla3600) into a 25mg / ml solution, stirred at room temperature for 12 hours.

[0064] (2) Take 2ml of the solution and dialyze in ultrapure water for 24 hours with a suspension dialysis bag. During this period, change the water every 6 hours to ensure that all the residues of the reaction are removed. The bag was dialyzed in ultrapure water for 24 hours, and the water was changed every 6 hours during the period. After the dialysis, the solution was placed in the refrigerator for standby, and it was named PC-PLEL.

[0065] 2. Fourier transform infrared spectroscopy detection

[0066] (1) Put the PC-DOX-PLEL and PC-PLEL freeze...

Embodiment 2

[0069] Example 2 Preparation of phycocyanin-polylactic acid-doxorubicin micelles (PC-DOX-PLEL micelles)

[0070] Synthesis schematic diagram of PC-DOX-PLEL micelles provided by the present invention is as attached figure 2 As shown, the details are as follows:

[0071] 1. Preparation of PC-DOX-PLEL micelles

[0072] Accurately weigh 0.2g of phycocyanin powder, dissolve it into 5 mg / mL phycocyanin solution with 10ml PBS (pH=7.4), stir well, then add 200μl of doxorubicin hydrochloride (1mg / ml), and stir for 30min , and then add 7.36ml DMSO to dissolve 0.184g polylactic acid copolymer (pla3600-peg2000-pla3600) into a 25mg / ml solution, and stir at room temperature for 12 hours.

[0073] Take 2ml of the solution and dialyze in ultrapure water for 24 hours with a suspension dialysis bag. During this period, change the water every 6 hours to ensure that all the residues of the reaction are removed. Dialyze in pure water for 24 hours, and change the water every 6 hours during the ...

Embodiment 3

[0095] Example 3 Anticancer application of PC-DOX-PLEL micelles

[0096] 1. C6 cell culture

[0097] The C6 cell line was provided by the Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences. After the cells were proliferated and cultured to 80% in the culture flask, they were seeded on a 24-well plate at a density of 16,000 / well, and cultured for 1-2 days for subsequent experiments. The cell culture conditions are: high-glucose DMEM medium containing 10% newborn calf serum, 37°C, 5.0% CO 2 .

[0098] 2. Trypan blue staining and cell counting

[0099] We chose the method of trypan blue staining and cell counting to preliminarily count the cell death rate after adding the drug, so as to preliminarily test the killing effect of phycocyanin-polylactic acid micelles on C6 brain cancer cells.

[0100] (1) Cultivate C6 cells to a normal state in a 24-well plate, so that the cells can fully adhere to the wall and grow. The experiment was divided into four group...

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Abstract

The invention discloses a cerebral cancer suppressing phycocyanin-polylactic acid-adriamycin micelle. Phycocyanin and PLA-PEG-PLA are utilized as raw materials, and a phycocyanin-polylactic acid no-load micelle is formed according to addition reaction of carboxyl and self-assembly principle based on hydrophilia and hydrophobicity. According to the self-assembly principle, adriamycin is coated in the no-load micelle to assemble by itself to form the micelle. The phycocyanin-polylactic acid-adriamycin micelle is a novel nano medicine capable of directly acting on tumor through blood-brain barrier to suppress cerebral cancer cells and capable of releasing slowly to retain in blood for a long time, thereby overcoming influence and limit of the blood-brain barrier to drug treatment effect. Besides, the micelle is prepared from natural or degradable components which are not involved with organic solvents during synthesis, has the advantages such as good biocompatibility, biodegradability, hypotoxicity, high loading capacity, and accordingly has high application potential in treating cerebral cancer.

Description

technical field [0001] The invention belongs to the technical field of medical materials. More specifically, it relates to a phycocyanin-polylactic acid-doxorubicin micelle for inhibiting brain cancer and its preparation method and application. Background technique [0002] Brain tumors refer to a heterogeneous population of primary and metastatic tumors of the central nervous system and are life-threatening diseases characterized by extremely low survival rates. According to statistics, the incidence of brain cancer is 40 / 100,000. In terms of the incidence of systemic tumors, brain tumors rank fifth (6.31%), only lower than stomach, uterus, breast, and esophagus tumors. Although the morbidity rate of the disease is not the highest, the fatality rate is very high. [0003] Current research shows that the treatment of malignant brain tumors includes surgical resection, radiotherapy, chemotherapy and combination therapy. These methods can greatly prolong the survival time o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/69A61K31/704A61K47/59A61P35/00
CPCA61K9/1075A61K31/704A61K47/42
Inventor 关燕清赵孟阳钟小飞
Owner SOUTH CHINA NORMAL UNIVERSITY
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