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O. verruca bzip1 gene

A wart grain wild rice, gene technology, applied in the field of plant biology, can solve the problems of resistant varieties becoming susceptible, frequent variation of physiological races, etc., to achieve the effect of enhancing disease resistance

Active Publication Date: 2018-11-30
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to frequent variation in bacterial blight races, new resistant varieties often become susceptible again after a few years of planting (Hulbert et al.2001)

Method used

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  • O. verruca bzip1 gene
  • O. verruca bzip1 gene
  • O. verruca bzip1 gene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] 1. Cloning of the OmbZIP1 gene of Oryza sativa

[0016] 1.1. RNA preparation of Oryza sativa

[0017] (1) Reagents:

[0018] Solution I ligase was purchased from TaKaRa Company; plasmid extraction kit was purchased from Beijing Kangwei Century Biotechnology Co., Ltd.; DNA fragment recovery kit was purchased from Promega Company; restriction endonuclease and cDNA first-strand synthesis kit were purchased from NEB company; TRIzol reagent was purchased from Invitrogen; high-fidelity DNA polymerase was purchased from TOYOBO; IPTG (Isopropyl-1thio-β-galactoside, isopropyl-thio-β-D-galactoside); X-gal (5 -bromo-4-chloro-3-indolyl-β-D-galactoside, 5-bromo-4-chloro-3-indole-β-thiogalactoside); Kanamycin (Kanamycin, Kan); Ampicillin Penicillin (Ampicillin, Amp); Streptomycin (Streptomycin, Str); Liquid nitrogen; Diethylprocarbonate (DEPC); Absolute ethanol; Chloroform; Isopropanol; Na2EDTA; Glacial acetic acid; Glycerin; Tryptone; Yeast extract; NaCl; agar were synthesized by...

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Abstract

The invention relates to the field of plant biotechnology, and discloses a bZIP1 gene of Oryza verruculata, which specifically includes the isolation, cloning and functional identification of a gene related to resistance to bacterial blight from Oryza verrucula, which is often contained in wild relatives of crops Disease resistance, stress resistance, high yield and other traits. Oryza sativa is almost immune to rice bacterial blight, and the resistance gene has not been effectively identified because it cannot hybridize with cultivated rice. The present invention clones and confirms a gene (OmbZIP1) in wild rice verruca, which is highly homologous to a bZIP-type transcription factor in cultivated rice. After the identification of the obtained transgenic materials, it was found that the gene can affect the resistance of rice to bacterial blight and enhance the resistance of rice to some bacterial blight races.

Description

technical field [0001] The present invention relates to the field of plant biotechnology, and relates to a Oryza verruculata bZIP1 gene, which specifically includes the isolation, cloning and functional analysis of a gene related to resistance to bacterial blight from Oryza verruculata, and in particular to the DNA sequence of Oryza verruculata. And the effect of the DNA sequence on rice resistance to bacterial blight. Background technique [0002] Rice is one of the most important food crops for human beings. High yield has always been an important agronomic trait for rice breeding selection, and it is also the main goal pursued by agricultural production. However, rice yield is often affected by biotic or abiotic stresses. Bacterial blight, caused by Xanthomonas oryzae pv. Oryzae, Xoo, is a serious global rice disease. Rice is the main food in many Asian countries, so rice bacterial blight poses a particularly serious threat to food production in Asian countries (Singh e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C12N15/82A01H6/46
CPCC07K14/415C12N15/8205C12N15/8281
Inventor 王栩鸣杨勇余初浪周洁严成其程晔陈剑平
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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