New application of fucoxanthin to preparation of medicine for treating alzheimer disease
A technology of Alzheimer's disease and fucoxanthin, applied in the field of fucoxanthin in the preparation of drugs for the treatment of Alzheimer's disease, can solve the problems of side effects of AChE inhibitors, achieve inhibition of Aβ fibrosis, and good safety , the effect of easy purification
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
specific Embodiment 1
[0019] Inhibition of acetylcholinesterase (AchE) by fucoxanthin
[0020] 1. Experimental materials and reagents
[0021] Fucoxanthin is extracted and purified from copper algae, and its chemical structure is as follows:
[0022] .
[0023] Experimental reagents: iodoacetylthiocholine (AChI) was purchased from sigma company, dissolved in 100mmol L -1 Na 2 HPO 4 solution, the concentration is 20mmol·L -1 . Dithiobis(2-nitrobenzoic acid) (DTNB) was purchased from LifeScience, dissolved in 100mmol L -1 Na 2 HPO 4 solution, the concentration is 5mmol L -1 . Ethylpromazine (BuChE inhibitor) was purchased from Sigma, dissolved in dimethyl sulfoxide (DMSO) at a concentration of 20 mmol L -1 .
[0024] Preparation of brain homogenate: the rats were decapitated and the brains were taken out, and the cerebral cortex was separated on ice and dried with liquid nitrogen. After weighing the cortex, add 10 times of lysis buffer (HEPES10mmol L -1 , TritonX-1000.5%, EDTA5mmol·L ...
specific Embodiment 2
[0037] Figure 5 The middle control group is untreated SH-SY5Y cells (from Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences), the cells added with hydrogen peroxide are the model group, and the cells added with different concentrations of fucoxanthin 2 hours in advance are the experimental group. Cell viability was detected by MTT colorimetry. Depend on Figure 5 It can be seen that adding hydrogen peroxide to SH-SY5Y cells can lead to cell death, and adding fucoxanthin 2 hours in advance can reduce the cell death rate caused by hydrogen peroxide. It is concluded that fucoxanthin can resist the cytotoxicity caused by oxidative stress. Have neuroprotective effect. Since Aβ may generate hydrogen peroxide in cells, which may cause oxidative stress and lead to cell death, this shows that fucoxanthin may produce neuroprotection against Aβ from a perspective.
[0038] Image 6 as well as Figure 7 The middle control group is untreated cells, the cells ad...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com