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Hydrogel applicable to cell adhesion and preparation method of hydrogel

A hydrogel and cell technology, applied in medical science, prosthesis, etc., can solve the problems of poor biocompatibility of hydrogel, damage to cells and tissues, weak mechanical properties, etc., and achieve easy control of synthesis conditions and biological phase. The effect of good capacitance and low synthesis cost

Inactive Publication Date: 2016-03-02
CHINA UNIV OF PETROLEUM (EAST CHINA)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the hydrogel formed by physical cross-linking generally has weak mechanical properties, and the cross-linked network is easily destroyed by changes in the external environment such as ionic strength, pH value, and temperature, which generally cannot meet the actual application needs of tissue engineering; Gels usually use traditional methods of synthesizing polymers or techniques such as photopolymerization and radiation polymerization to initiate copolymerization or polycondensation reactions to generate covalent bonds to form a covalently crosslinked network.
In the preparation process, it is often necessary to use a large amount of cytotoxic additives such as photoinitiators, crosslinking agents, and organic solvents, resulting in poor biocompatibility of the hydrogel. At the same time, the photoinitiation method without an initiator is not only relatively efficient Low, and local overheating can damage surrounding cells and tissues

Method used

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  • Hydrogel applicable to cell adhesion and preparation method of hydrogel
  • Hydrogel applicable to cell adhesion and preparation method of hydrogel
  • Hydrogel applicable to cell adhesion and preparation method of hydrogel

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] The preparation method of hydrogel of the present invention specifically comprises the following steps:

[0074] The above-prepared Ac-IIISLGK-NH 2 It is mixed with L-glutamine at a molar ratio of 1:1 and synthesized under enzymatic reaction conditions. During the enzymatic reaction, Ac-IIISLGK-NH 2 The final concentration of the solution was 7.27 mM, the concentration of transglutaminase TG was 0.9 U / mL, and the reaction temperature was controlled at 37° C. for 24 hours to obtain a hydrogel.

[0075] Detection: Take 10 μL of hydrogel sample dropwise on the surface of a new mica sheet, after static adsorption for 10s, blow dry the sample with high-purity nitrogen, AFM scanning, take a complete piece of hydrogel on the surface of the sealing film, place the carbon film A 400-mesh copper mesh was covered on the top of the gel and allowed to absorb for 1 min. After removing the copper mesh, use filter paper to absorb the residual liquid around the copper mesh. Stain with ...

Embodiment 2

[0077] The preparation method of hydrogel of the present invention specifically comprises the following steps:

[0078] 500 μL of Ac-IIISLGK-NH 2 (16mM) and L-Q (16mM) mixed solution at room temperature for 24 hours, add 25μL TG solution and the same amount of Ca 2+ The dependent solution, after being thoroughly mixed, was placed in a water bath at 37°C for 24 hours to obtain a hydrogel.

[0079] Mechanical strength detection of the hydrogel: the mechanical properties (viscoelasticity) of the gel formed by the hydrogel catalyzed by transglutaminase were characterized by a Haake rheometer, and the measuring module used was a cone with a diameter of 35 mm and a taper of 2°. The plate and the corresponding sample loading platform, the sample volume for each measurement is 500 μL, the temperature of the rheological experiment is 25 ° C, and after the sample reaction is catalyzed by transglutaminase for 24 hours, the stress scan is performed at a frequency of 1 Hz, and the scan ra...

Embodiment 3

[0088] The preparation method of hydrogel of the present invention specifically comprises the following steps:

[0089] The above-prepared Ac-IIISLGK-NH 2 It is mixed with L-glutamine at a molar ratio of 1:1 and synthesized under enzymatic reaction conditions. During the enzymatic reaction, Ac-IIISLGK-NH 2 The final concentration of the solution was 16 mM, the concentration of transglutaminase TG was 0.9 U / mL, and the reaction temperature was controlled at 38° C. for 36 hours to obtain a hydrogel.

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Abstract

The invention discloses hydrogel applicable to cell adhesion and a preparation method of the hydrogel. The hydrogel is formed by Ac-IIISLGK-NH2 and L-Glutamine (L-Gln) under the action of an enzymatic reaction, wherein the N-terminal of the Ac-IIISLGK-NH2 is acetylated, the C-terminal of the Ac-IIISLGK-NH2 is aminated, the mole ratio of the Ac-IIISLGK-NH2 to the L-Gln is 1:1, reaction temperature is 37-40 DEG C, and reaction time is 24-48 hours. The hydrogel has the advantages that the prepared hydrogel can allow cells to be well adhered to cell surfaces, is low in toxicity and good in biocompatibility, does not cause the non-specific immunity reaction of lymphocyte, is ideal nano tissue engineering material and is significant to human life health.

Description

technical field [0001] The invention relates to the technical field of hydrogel preparation, in particular to a hydrogel that can be used for cell adhesion and a preparation method thereof. Background technique [0002] Ideal tissue engineering materials not only need to have a grid structure that supports and connects tissues, but also need to have advantages in regulating tissue generation and physiological activities of cells. At present, most scaffold materials use biomacromolecules (chitosan, alginate, collagen) or synthetic macromolecules (polyethylene glycol), etc., to provide a microenvironment to support cell growth. However, problems such as chemical residues, disease source transmission and synthesis cost limit its in-depth application. [0003] Transglutaminase in nature widely exists in the human body, advanced animals, plants and microorganisms, and can catalyze the acyl transfer reaction, which can catalyze the relationship between the γ-carbonamide of glutam...

Claims

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Application Information

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IPC IPC(8): A61L27/22A61L27/52
Inventor 陈翠霞徐海王景新张宇白景琨
Owner CHINA UNIV OF PETROLEUM (EAST CHINA)
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