Method for preparing bacterial cellulose from soybean molasses

A technology of bacterial cellulose and soybean molasses is applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., and can solve the problems of incapable of industrialized production and popularization, high production cost, low bacterial cellulose yield, etc. Effects of high productivity, reduced production cost, and shortened culture time

Inactive Publication Date: 2016-02-24
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, bacterial cellulose has problems such as low yield and high production cost, which prevent it from being industrialized and popularized.

Method used

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  • Method for preparing bacterial cellulose from soybean molasses
  • Method for preparing bacterial cellulose from soybean molasses

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] (1) Seed cultivation

[0019] The bacteria were inoculated in: glucose, 5g / L, NaCl, 5g / L, beef extract, 10g / L, yeast extract powder, 10g / L, pH value of 6.8 medium, at 180r / min, 30℃ Cultivate under the conditions for 24 hours to obtain seed liquid, and the strain is Acetobacter xylinum.

[0020] (2) Preparation of fermentation medium

[0021] Dilute the soybean molasses with water to make the concentration 10%, then adjust the pH value to 3.0 with 4mol / L sulfuric acid, acidify at 100°C for 60min, centrifuge, and take the supernatant;

[0022] According to the content of corn gluten powder per liter of the medium, 10g, Na 2 HPO 4 , 2g, citric acid, 1g, MgSO 4 , 0.1g, and the rest is supplemented with processed soybean molasses, and the pH value is adjusted to 7.

[0023] (3) Preparation of bacterial cellulose

[0024] After the Acetobacter xylinum seed liquid was fermented for 24 hours, inoculate it in the fermentation medium according to the inoculum amount of 10%,...

Embodiment 2

[0028] (1) Seed cultivation

[0029] The bacteria were inoculated in: glucose, 5g / L, NaCl, 5g / L, beef extract, 10g / L, yeast extract powder, 10g / L, pH value of 6.8 medium, at 180r / min, 30℃ Cultivate under the conditions for 24 hours to obtain seed liquid, and the strain is Acetobacter xylinum.

[0030] (2) Preparation of fermentation medium

[0031] Dilute the soybean molasses with water to make the concentration 20%, then adjust the pH value to 3.0 with 4mol / L sulfuric acid, acidify at 100°C for 60min, centrifuge, and take the supernatant;

[0032] According to the content of corn gluten powder per liter of the medium, 10g, Na 2 HPO 4 , 2g, citric acid, 1g, MgSO 4 , 0.1g, and the rest is supplemented with processed soybean molasses, and the pH value is adjusted to 7.

[0033] (3) Preparation of bacterial cellulose

[0034] After the Acetobacter xylinum seed liquid was fermented for 24 hours, inoculate it in the fermentation medium according to the inoculum amount of 10%,...

Embodiment 3

[0038] (1) Seed cultivation

[0039] The bacteria were inoculated in: glucose, 5g / L, NaCl, 5g / L, beef extract, 10g / L, yeast extract powder, 10g / L, pH value of 6.8 medium, at 180r / min, 30℃ Cultivate under the conditions for 24 hours to obtain seed liquid, and the strain is Acetobacter xylinum.

[0040] (2) Preparation of fermentation medium

[0041] Dilute soybean molasses with water to make the concentration 30%, then adjust the pH value to 3.0 with 4mol / L sulfuric acid, acidify at 100°C for 60min, centrifuge, and take the supernatant;

[0042] According to the content of corn gluten powder per liter of the medium, 10g, Na 2 HPO 4 , 2g, citric acid, 1g, MgSO 4 , 0.1g, and the rest is supplemented with processed soybean molasses, and the pH value is adjusted to 7.

[0043] (3) Preparation of bacterial cellulose

[0044] After the Acetobacter xylinum seed liquid was fermented for 24 hours, inoculate it in the fermentation medium according to the inoculum amount of 10%, fer...

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Abstract

The invention relates to a method for producing bacterial cellulose from soybean molasses serving as a cheap raw material. The soybean molasses are pretreated and taken as a main carbon source, the bacterial cellulose is prepared with a two-step method, and the two-step method comprises shake cultivation and transferring to a shallow plate for standing fermentation. A new raw material is provided for production of the bacterial cellulose. Raw materials are widely sourced, the cost is low, the equipment method is simple, the cultivation time is short, the yield of the bacterial cellulose is high, and the application prospect is wide.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation production, in particular to a method for producing bacterial cellulose with soybean molasses as the main raw material. Background technique [0002] Bacterial cellulose is a high-molecular polymer formed by some bacteria that is secreted out of the cell through heterotrophic production, and glucose is linked by β-1,4 glycosidic bonds. Compared with plant cellulose, it has the advantages of ultrafine, high purity, strong biological adaptability, high degradability, high Young's modulus, high hardness, high crystallinity, etc., so it is widely used in food, papermaking, non-woven, sound Equipment, oil fields, biomedical materials, and fuel cells are emerging materials that have attracted much attention at home and abroad. However, due to the long fermentation period, high cost and low yield of bacterial cellulose produced by traditional fermentation, the further application of bacte...

Claims

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Application Information

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IPC IPC(8): C12P19/04C12R1/01
CPCC12P19/04
Inventor 徐速程建军尹园马佳歌张珊葛小源
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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