Anti-LKM (liver-kidney microsomal) 1 antibody detection kit and detection method

Abstract

The invention discloses an anti-LKM (liver-kidney microsomal) 1 antibody detection kit and a detection method. The kit comprises biotinylation LKM-1 antigen, a calibrator, a quality control material, an AP (alkaline phosphatase)-anti-human IgG (intravenous gamma globulin) antibody, a magnetic particle reagent, a chemiluminescent substrate and a cleaning liquid. According to the detection method, the reagent in the kit is used for detection of the sample. With the adoption of the scheme, a full-automatic magnetism particulate chemiluminescent detection method is adopted for selection of an alkaline phosphatase AP-adamantane AMPPD system, and the kit has the advantages of good stability, high sensitivity, good repeatability and the like; meanwhile, the detection time is greatly shortened, the total time required for one test is about 50 minutes, the operation is simple and convenient, and full-automatic detection is really realized.

Description

technical field [0001] The invention relates to the technical field of magnetic particle chemiluminescence immunodiagnosis, in particular to an anti-liver-kidney microsome 1 antibody detection kit and detection method. Background technique [0002] In recent years, the research on autoimmune liver disease at home and abroad has become more and more in-depth, and has progressed rapidly, involving pathogenesis, clinical diagnosis, clinical treatment, prognosis, genetics, and research on related autoantibodies and target antigens. [0003] Liver-kidney microsomal (LKM) autoantibodies are related to liver inflammatory diseases, and LKM autoantibodies are related to autoimmune chronic active hepatitis, also known as LKM-1 autoantibodies. LKM-1 is a protein with a molecular weight of 50KDa, which was first discovered in rat liver as cytochrome P450db1. Recent studies have reported that three P450 cytochromes ( A2, D6 and C9) can be used as an autoantigen for hepatitis disea...

AI Technical Summary

Problems solved by technology

[0009] (1) False positives may occur using this method

[0010] (2) When analyzing the results, non-specific recognition cannot be distinguished according to the size of the molecular weight

[0011] (3) The operation is relatively complicated and requires a more expensive fluorescence microscope, which is difficult to promote in many primary hospitals and is not suitable for laboratories with a large number of specimens

[0012] (4) The background in the fluorescence measurement is high, and it is difficult to use the fluorescence immunoassay technique for quantitative determination

[0013] (5) Experienced professionals are required to judge the results, and the objectivity of the analysis results is insufficient

[0016] (1) The detection reagent is an open method during the detection process, which may easily cause cross-contamination between various reagents and affect the detection results

[0017] (2) ELISA methods mostly use horseradish peroxidase for detection, and its detection range and sensitivity are low

[0018] (3) The detection time of the ELISA method is usually relatively long, and the total time required to complete a test is generally more than 2 hours, which cannot fully meet the needs of clinical rapid diagnosis

[0019] (4) The ELISA method cannot be randomly injected for detection, and there is a lag in the detection results

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