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Green feed additive

A green feed and additive technology, applied in animal feed, animal feed, application, etc., can solve the problems of low pig absorption rate, poor compatibility, and environmental pollution, and achieve good compatibility, low environmental pollution, and high absorption rate.

Inactive Publication Date: 2016-01-06
BEIJING KEHUITONG WISDOM SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are many problems in adding this inorganic copper sulfate to the feed, such as low absorption rate of pigs, poor compatibility with other components in the diet, high excretion rate, and environmental pollution.

Method used

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  • Green feed additive

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1: Acquisition of marine Rhodotorula yeast WYN1 and domestication of copper tolerance

[0034] (1) Fresh sea shrimp, starfish, seaweed and other biological samples collected from the Yellow Sea are placed in sterile YEPD medium, and red or pink round colonies are picked for further purification and culture. According to the accumulation of carotenoids in each strain, the strains with high carotenoid production were screened. Among them, the marine rhodotorula WYN1 with higher carotenoid production has oval cells without pseudohyphae; it forms red colonies on the YEPD medium prepared in seawater, with smooth surface and neat edges; it does not produce eggs on the sporulation medium Cystic spores, multilateral budding, no sporulation; no fermentation of sugar, no formation of starch-like compounds, and no inositol synthesis. Based on the above characteristics, it was preliminarily determined to belong to the genus Rhodotorula. Keep ramps in the refrigerator.

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Embodiment 2

[0051] Example 2: (Comparative Test) Fermentation Conditions for High-yielding Carotenoids of Rhodotorula marina WYN1

[0052] Fermentation medium composition: glucose 2.5%, peptone 2.5%, magnesium sulfate 0.02%, potassium dihydrogen phosphate 0.15%, copper content 300mg / L (copper sulfate configuration), sodium chloride 20%, the rest is water; the initial pH is 8 , the inoculum amount was 10%, the shaker speed was 180r / min, and after culturing at 28°C for 24 hours, the temperature was adjusted to 30°C, the pH was adjusted to 5, and the nitrogen source was added once, that is, peptone was added according to the addition amount of 2.5%, and continued Ferment for 24h. The marine Rhodotorula WYN1 was fermented and cultured by the above method, and its biomass, carotenoid production and intracellular copper content were 27.52 g / L, 25.12 mg / L and 6240 μg / g dry bacteria, respectively.

Embodiment 3

[0053] Embodiment 3: feed additive and its preparation

[0054] The parts by weight of the feed additive are composed as follows:

[0055] Compound polysaccharide extract 10, marine red yeast fermented culture 15, eucommia leaf extract 6, licorice 5, tamarind 5, houttuynia cordata 2, dandelion 3; the marine red yeast preservation number is CCTCCNO: M2014592.

[0056] The preparation method of described complex polysaccharide extract is as follows:

[0057] The raw materials astragalus, alfalfa and wolfberry were dried and crushed through a 60-mesh sieve at a mass ratio of 4:2:1, and boiling water was added to 8 times the total weight of the crushed matter, extracted twice for 30 minutes each time, filtered, mixed with the extracts, and cooled naturally to At room temperature, add absolute ethanol to the extract solution to make the ethanol concentration in the mixture reach 70%, seal and refrigerate for precipitation at 10°C for 8 hours, dry the precipitate in vacuum, crush i...

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Abstract

The present invention provides a green feed additive which is composed of the following components in parts by weight: 8-15 parts of compound polysaccharide extract, 8-20 parts of marine red yeast fermentation cultures, 5-10 parts of folium cortex eucommiae extract, 3-8 parts of licorice root, 3-8 parts of tamarind fruit, 1-5 parts of herba houttuyniae and 1-5 parts of dandelions. The compound polysaccharide extract is from radix astragali, Chinese wolfberry fruits and medicago sativa. The preservation number of the marine red yeast is CCTCC No: M 2014592. The marine red yeast can not only produce high-yield carotenoid, but also enrich copper, through the static culture method, the carotenoid yield and copper content in the yeast are improved, and the marine red yeast is used as feed additive to be applied to feed and can provide carotenoids, B vitamins and copper ions. The marine red yeast well replaces the inorganic copper ions used in the conventional feed due to high-efficiency copper enriching ability and the copper ions provided by the marine red yeast are good in stability. In combination with a variety of Chinese herbal medicines, the feed additive can replace antibiotics to be used in feed, improve the safety of pork products, improve feed utilization efficiency, enhance animal organism immunity, regulate intestinal health, improve animal metabolism function and reproductive capacity, and improve breeding efficiency.

Description

technical field [0001] The invention belongs to the field of feed additives, and in particular relates to a green feed additive product capable of increasing feed utilization and improving animal intestinal health and a preparation method thereof. Background technique [0002] At present, the aquaculture industry generally has problems such as low feed utilization rate, incomplete digestion of nutrients, and poor storage that lead to moisture absorption, mildew or insects in the feed. The existence of these problems not only greatly increases the cost of feeding, but also leads to waste of resources. [0003] Solving the above problems, controlling the cost of breeding, and reducing the risk of breeding are a major bottleneck for the rapid development of the breeding industry. The use of chemically synthesized additives will cause problems such as animal durability and residues, endangering human health, and there will be a large amount of excrement, such as ammonia, hydroge...

Claims

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Application Information

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IPC IPC(8): A23K1/14A23K1/16A23K1/00
Inventor 李绩
Owner BEIJING KEHUITONG WISDOM SCI & TECH CO LTD
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