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Preparation of carbon quantum dots having catalysis performance, and applications of kit based on carbon quantum dots

A catalytic performance and kit technology, applied in the field of chemical applications, can solve problems such as personal and social hazards, and achieve the effects of obvious phenomena, high sensitivity and good stability

Active Publication Date: 2015-12-23
HUNAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

A large number of facts show that the abuse of methamphetamine will bring serious harm to individuals and society, and it is urgent to establish an ultra-high sensitivity and rapid analysis method for methamphetamine

Method used

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  • Preparation of carbon quantum dots having catalysis performance, and applications of kit based on carbon quantum dots
  • Preparation of carbon quantum dots having catalysis performance, and applications of kit based on carbon quantum dots
  • Preparation of carbon quantum dots having catalysis performance, and applications of kit based on carbon quantum dots

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Embodiment 1

[0031]Take 0.5g of polyethyleneimine, 0.1g of cysteine, 10mL of ethylene glycol, and 2mL of distilled water in a 50mL beaker, add 4g of solid phosphoric acid, and heat in an oil bath (180°C) until the solution is light yellow gel , add 2 mL of secondary water; repeat this step 5 times until a brown gel is formed, and cool to room temperature naturally. Then dissolve the gel with 20 mL of secondary water, and adjust the pH of the solution to ≈7 with 10 mol / L NaOH solution. Add another 50mL of absolute ethanol, let it stand for 12h, and the solution is separated. Aspirate the supernatant, remove the insoluble matter in the lower layer, and obtain the ethanol solution of carbon dots. The ethanol solution of carbon dots is subjected to rotary evaporation treatment, and rotated to almost no liquid state to obtain solid carbon dots. Dissolve solid carbon dots with secondary water, and dialyze with a dialysis bag with a molecular weight cut-off of 1000 to obtain a pure carbon dot s...

Embodiment 2

[0033] Chromogenic reaction to breast cancer antigen. Breast cancer antigen standard solution, that is, the sample diluent, is to dilute the pure breast cancer antigen standard product with pH=7.2-7.4 and PBS buffer solution with a concentration of 0.01mol / L into solutions of different concentrations; the carbon dot-labeled breast cancer antibody is After activating the carbon dots with activators EDC and NHS, breast cancer antibodies were added to the activated carbon dot solution, reacted for 1 hour, and dialyzed for 24 hours. First, breast cancer antibody was added to a 96-well polystyrene microwell plate, reacted overnight at 4°C, washed three times with PBS buffer solution, added BSA solution and reacted for 1 hour, washed three times with PBS buffer solution, and then added different concentrations of breast cancer Antigen standard solution (1×10 -12 to 1×10 -14 U / mL) reaction for 1 hour, after washing with PBS buffer solution three times again, add the breast cancer a...

Embodiment 3

[0035] Take 5 serum samples from breast cancer patients, firstly add breast cancer antibody to 5 wells of 96-well polystyrene microwell plate, react overnight at 4°C, wash with PBS buffer solution three times, add BSA solution and react for 1 hour, Wash three times with PBS buffer solution and add dilution 10 10 times human serum samples. Reaction for 1 hour, after washing with PBS buffer solution three times again, add breast cancer antibody that has been connected to carbon dots for reaction for 1 hour, also wash with PBS buffer solution three times, add 100 μL of 4 mM trisodium citrate solution and 100 μL of 320 μmol / L hydrogen peroxide solution was reacted for 40 minutes, and then 100 μL of chloroauric acid solution with a concentration of 2 mmol / L was added. After 30 minutes, its color changes as Figure 4 As shown, when the concentration of breast cancer antigen is 0, the color of gold nanoparticles in the microwell plate is red, and in the microwell plate after addin...

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Abstract

The present invention discloses preparation of carbon quantum dots having catalysis performance, and applications of a kit based on the carbon quantum dots. The carbon quantum dot preparation mainly comprises: carrying out oil bath heating on polyethyleneimine, cysteine, ethylene glycol, distilled water and solid phosphoric acid to obtain a pale yellow gel, dissolving with water, and naturally cooling to a room temperature; and treating the obtained brown gel product to obtain the product. The applications of the kit based on the carbon quantum dots mainly comprise: the characteristic that the carbon quantum dots catalyze and decompose hydrogen peroxide to organically combine two nanometer materials such as the carbon quantum dots and the gold nanoparticles, and the carbon quantum dots have biological enzyme property and the antibody and the antigen are specifically bound to form a sandwich structure so as to achieve the detection of breast cancer antigen and the hypersensitivity visual detection of ice drug. According to the present invention, the phenomenon is obvious, the operation is easy, the sensitivity is ultra-high, and the breast cancer antigen concentration in the serum and the ice drug content in human serum, saliva and urine can be effectively and accurately determined.

Description

technical field [0001] The invention belongs to the technical field of chemical application, and in particular relates to the preparation of a carbon dot with catalytic performance and the application of the carbon dot kit based on the carbon dot. Background technique [0002] As a new functional nanomaterial, carbon dots have good characteristics, such as strong fluorescence, good biocompatibility, low cytotoxicity, small particle size, simple synthesis method, and safe operation. In view of the excellent fluorescence properties of carbon dots, easy functionalization and industrialization, and non-toxic properties, it will bring new development space to the fields of luminescent materials, optoelectronic devices, and green biomedicine in the future, such as for biological imaging, biochemical analysis and detection. , optoelectronic devices, photocatalysis, metal ion detection and two-photon imaging, etc. In addition, some carbon dots exhibit good photocatalytic performanc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/533
CPCG01N33/533
Inventor 黄昊文张凌阳刘兰芳周媛
Owner HUNAN UNIV OF SCI & TECH
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