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An electrochemiluminescent method for detecting graphene oxide

An electrochemical and graphene technology, applied in the field of electrochemical detection, can solve the problems of high technical cost and long processing time, and achieve the effects of high sensitivity, short detection time and low cost

Inactive Publication Date: 2018-04-24
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, detection methods for graphene oxide such as fluorescence, chromatography, etc. have been developed. Due to the high cost of these techniques, long processing time, and the need for technical skills and complex equipment requirements, a fast, simple, high-sensitivity, An easy-to-use method is necessary

Method used

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  • An electrochemiluminescent method for detecting graphene oxide
  • An electrochemiluminescent method for detecting graphene oxide

Examples

Experimental program
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Effect test

Embodiment 1

[0025] In the electrochemiluminescence cell that 4mL 0.1M PBS (pH8) is housed, add the 0.1M sodium hydroxide solution that contains 0.01M luminol, after mixing, obtain mixed solution A, wherein the concentration of luminol is 0.01mM, The mixed solution A is scanned for the electrochemiluminescence signal through a three-electrode system at a voltage of -0.3V to 0.8V, a scan rate of 100mV / s, and a photomultiplier tube voltage of 800V to obtain the luminescence of luminol Signal;

[0026] Add different amounts of graphene oxide to mixed solution A in turn, fully dissolve, and mix well to obtain mixed solution B with different GO concentrations; in mixed solution B, GO concentrations are 2.4 μg / mL, 8 μg / mL, and 16 μg / mL , 36 μg / mL, 48 μg / mL, 64 μg / mL, 88 μg / mL, 160 μg / mL, 240 μg / mL, 300 μg / mL, 320 μg / mL and 350 μg / mL, the mixed solution B was passed through a three-electrode system at a voltage of - Under the parameters of 0.3V~0.8V, scan rate of 100mV / s, and photomultiplier tub...

Embodiment 2

[0028] In the electrochemiluminescence cell that 4mL 0.1M PBS (pH9) is housed, add the 0.1M sodium hydroxide solution that contains 0.01M luminol, after mixing, obtain mixed solution A, wherein the concentration of luminol is 0.05mM, The mixed solution A is scanned for the electrochemiluminescence signal through a three-electrode system at a voltage of -0.3V to 0.8V, a scan rate of 100mV / s, and a photomultiplier tube voltage of 800V to obtain the luminescence of luminol Signal;

[0029] Add different amounts of graphene oxide to mixed solution A in turn, fully dissolve, and mix well to obtain mixed solution B with different GO concentrations; in mixed solution B, GO concentrations are 0.25 μg / mL, 0.56 μg / mL, 0.8 μg / mL, 2.4μg / mL, 8μg / mL, 16μg / mL, 36μg / mL, 48μg / mL, 64μg / mL, 88μg / mL, 160μg / mL, 240μg / mL, 400μg / mL and 500μg / mL, for The mixed solution B scans the electrochemiluminescent signals of the mixed solutions with different concentrations of GO through the three-electrode ...

Embodiment 3

[0031] In the electrochemiluminescence cell that 4mL 0.1M PBS (pH9) is housed, add the 0.1M sodium hydroxide solution that contains 0.01M luminol, after mixing, obtain mixed solution A, wherein the concentration of luminol is 0.1mM, The mixed solution A is scanned for the electrochemiluminescence signal through a three-electrode system at a voltage of -0.3V to 0.8V, a scan rate of 100mV / s, and a photomultiplier tube voltage of 800V to obtain the luminescence of luminol Signal;

[0032] Add different amounts of graphene oxide to mixed solution A in turn, fully dissolve, and mix well to obtain mixed solution B with different GO concentrations; in mixed solution B, GO concentrations are 0.08μg / mL, 0.24μg / mL, 0.56μg / mL, 0.8μg / mL, 2.4μg / mL, 8μg / mL, 16μg / mL, 36μg / mL, 48μg / mL, 64μg / mL, 88μg / mL, 160μg / mL, 240μg / mL, 400μg / mL, 560μg / mL and 800μg / mL, for the mixed solution B through the three-electrode system, the voltage is -0.3V ~ 0.8V, the scan rate is 100mV / s, and the photomultipl...

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Abstract

The invention discloses an electrochemiluminescence method for detecting graphene oxide. First, PBS buffer solution is added to the electrochemiluminescence cell, and then luminol solution is injected into the solution to obtain a very strong luminol ECL intensity. . Finally, when different concentrations of GO were added to PBS buffer solution containing luminol, different degrees of quenching of luminol ECL were obtained. Based on the inhibitory effect of GO on the ECL of luminol, the ECL signal of luminol was reduced; then it was used for simple, rapid and sensitive detection of GO. In the concentration range of 0.08-800 μg / mL, the logarithm of GO concentration and the intensity value of △IECL showed a good linear relationship, and the detection limit could reach 0.0267 μg / mL. In addition, the sample required by this method is easy to synthesize, the detection is simple, the time is short, and the sensitivity is high. It is expected to be applied to the detection and analysis of GO in actual samples.

Description

technical field [0001] The invention relates to an electrochemiluminescent method for detecting graphene oxide. The method has the characteristics of fast, simple and sensitive, and belongs to the technical field of electrochemical detection. Background technique [0002] Due to the continuous production of graphene and the massive use of graphene and graphene-family nanomaterials, there is a need to assess its impact on the environment, ecosystem health, and human life. Graphene oxide (GO) nanoparticles can be discharged into the environment through the atmosphere and waste materials generated in production and research and development. Nanoparticles in the environment can contaminate soil surfaces and groundwater and it can also bind to organic organisms. However, detection methods for graphene oxide such as fluorescence, chromatography, etc. have been developed. Due to the high cost of these techniques, long processing time, and the need for technical skills and complex ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/76
Inventor 王坤费爱荣刘倩还娟钱静
Owner JIANGSU UNIV
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