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Preparation method of dermal tissue bionic porous scaffold

A porous scaffold and tissue technology, applied in the field of medical bionic materials, can solve the problems of unstable quality of acellular dermal matrix bionic dermis, virus transmission, secondary surgery, etc., to improve adhesion and proliferation activity, and improve biocompatibility , The effect of promoting biomimetic performance

Active Publication Date: 2015-11-18
WUXI NO 3 PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

First of all, due to the difficult control of the processing technology, the quality of biomimetic dermis based on acellular dermal matrix is ​​unstable, and there are often quality differences between production batches, which will affect its application effect as a dermal substitute for wound repair, and may cause secondary damage. Serious consequences such as surgery
Secondly, the acellular dermal matrix bionic dermis has the possibility of biological contamination, because the raw human cadaver skin may carry viruses, and these viruses are not effectively removed during material processing, resulting in the spread of viruses
Thirdly, the degradation rate of in vitro synthetic bionic dermis on the wound surface does not match the rate of tissue generation, and its effect of promoting wound repair and inhibiting scar formation is often weakened due to too fast degradation
In addition, the effect of the existing bionic dermis on inhibiting scar formation is far from reaching the goal of promoting the regeneration of functional dermis-like tissue

Method used

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  • Preparation method of dermal tissue bionic porous scaffold
  • Preparation method of dermal tissue bionic porous scaffold
  • Preparation method of dermal tissue bionic porous scaffold

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A preparation method of dermal tissue bionic porous scaffold, the specific preparation steps are:

[0026] (1) Put 10g of silkworm cocoons in 500ml of 0.02MNa 2 CO 3 Boil in aqueous solution for 30 minutes, then rinse with deionized water 3 times to obtain silk fibroin fiber; then place silk fibroin fiber in 10ml of 10M lithium bromide solution and dissolve it at 37°C to obtain silk fibroin lithium bromide solution; finally After the obtained solution was dialyzed in a dialysis cassette with a molecular weight cutoff of 3500 Da for 3 days, a silk protein solution was obtained, and the mass percentage concentration of the solution was adjusted to 5%;

[0027] (2) Mix the silk protein solution prepared in step (1) with the alginic acid solution with a mass percentage concentration of 2% at a volume ratio of 10:10 at 37°C, and on a vortex shaker with a speed of 50 rpm / min Shake for 5 minutes until the mixture is uniform, then centrifuge for 3 minutes at a centrifugal force of 1...

Embodiment 2

[0033] A preparation method of dermal tissue bionic porous scaffold, the specific preparation steps are:

[0034] (1) Put 10g of silkworm cocoons in 500ml of 0.05MNa 2 CO 3 Boil in the aqueous solution for 30 minutes, then rinse with deionized water 3 times to obtain silk fibroin fiber; then place the silk fibroin fiber in 10ml of 20M lithium bromide solution and dissolve it at 37°C to obtain a silk fibroin lithium bromide solution; finally After the obtained solution was dialyzed in a dialysis cassette with a molecular weight cutoff of 3500 Da for 3 days, a silk protein solution was obtained, and the mass percentage concentration of the solution was adjusted to 5%;

[0035] (2) Mix the silk protein solution prepared in step (1) with the alginic acid solution with a mass percentage concentration of 2% at a volume ratio of 36:10 at 37°C, and on a vortex shaker with a speed of 50rpm / min Shake for 5 minutes until the mixture is uniform, then centrifuge for 3 minutes at a centrifugal f...

Embodiment 3

[0040] A preparation method of dermal tissue bionic porous scaffold, the specific preparation steps are:

[0041] (1) Put 10g of silkworm cocoons in 500ml of 1MNa 2 CO 3 Boil in the aqueous solution for 30 minutes, then rinse with deionized water 3 times to obtain silk fibroin fiber; then place the silk fibroin fiber in 10ml of 1M lithium bromide solution and dissolve it at 37°C to obtain a silk fibroin lithium bromide solution; finally After the obtained solution was dialyzed in a dialysis cassette with a molecular weight cutoff of 3500 Da for 3 days, a silk protein solution was obtained, and the mass percentage concentration of the solution was adjusted to 5%;

[0042] (2) Mix the silk protein solution prepared in step (1) with the alginic acid solution with a mass percentage concentration of 2% at a volume ratio of 4:10 at 37°C, and on a vortex shaker with a speed of 50rpm / min Shake for 5 minutes until the mixture is uniform, then centrifuge for 3 minutes at a centrifugal force ...

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Abstract

The invention discloses a preparation method of a dermal tissue bionic porous scaffold. The preparation method comprises the steps of: mixing a fibroin solution with alginic acid solution, then performing water vapor evaporation to form a membrane, incubating protein / alginic acid polymeric membrane in a calcium chloride aqueous solution, and finally drying to prepare the dermal tissue bionic porous scaffold. The dermal tissue bionic porous scaffold material disclosed by the invention can effectively promote wound repair and inhibit scar formation, and has a very good curative effect on open wounds including burn wounds and the like.

Description

Technical field [0001] The invention relates to the technical field of medical biomimetic materials, in particular to a method for preparing a biomimetic porous scaffold of dermal tissue by using silk protein and alginic acid as raw materials. Background technique [0002] Skin defects caused by open wounds such as burns are common and frequently-occurring clinical diseases. In the process of skin defect repair, excessive wound healing will lead to the occurrence and development of pathological scars. Pathological scars include hypertrophic scars and keloids, which can cause a series of serious complications such as itching, disfigurement, and disability, and sometimes even induce malignant tumors, which seriously reduce the quality of life of patients. Therefore, the prevention and treatment of pathological scars has always been an urgent medical problem in the field of surgery such as burns and plastic surgery. [0003] In recent years, with the continuous in-depth study of woun...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/22A61L27/20A61L27/60A61L27/56
Inventor 赵朋吕国忠
Owner WUXI NO 3 PEOPLES HOSPITAL
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