Glutathione-Au/Ag alloy nano material, preparation method and application thereof

A glutathione, gold-silver alloy technology, applied in nanotechnology, luminescent materials, nanotechnology and other directions, can solve the problems of complex process, unenvironmental protection, difficult control of stability and uniformity, etc., to achieve stable and uniform structure, avoid The effect of simple and convenient use and preparation of toxic reagents

Active Publication Date: 2015-11-11
SHANTOU UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to provide a preparation method of glutathione-gold-silver alloy nanomaterials to solve the problems that the stability and uniformity of the existing preparation methods are difficult to control, the process is complex and not environmentally friendly

Method used

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  • Glutathione-Au/Ag alloy nano material, preparation method and application thereof
  • Glutathione-Au/Ag alloy nano material, preparation method and application thereof
  • Glutathione-Au/Ag alloy nano material, preparation method and application thereof

Examples

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Effect test

Embodiment 1

[0048]The preparation method of glutathione-gold-silver alloy nanomaterial is as figure 1 shown, including the following steps:

[0049] Step 1: Add 2.5 mL of 100 mM silver nitrate solution and 2.5 mL of 100 mM trisodium citrate solution into 39 mL of deionized water and stir evenly.

[0050] Step 2: Add 6 mL, 50 mM freshly prepared sodium borohydride solution into the mixed solution in Step 1, and stir continuously for 14 hours to obtain a silver nanoparticle solution.

[0051] Step 3: Add 307.3 mg of glutathione powder into the silver nanoparticle solution obtained in step 2, then react the mixture in an oil bath for 50 hours at 70° C. to obtain a light orange solution, which is stabilized with glutathione solution of silver nanoparticles.

[0052] Step 4, add 4 mL of the glutathione-stabilized silver nanoparticle solution of step 3 into 10.8 mL of deionized water, and stir evenly; then slowly add 1.2 mL, 10 mM chloroauric acid solution (HAuCl 4 4H 2 O), continue to stir...

Embodiment 2

[0056] The preparation method of glutathione-gold-silver alloy nanomaterial is as figure 1 shown, including the following steps:

[0057] Step 1: Add 2.64 mL of 100 mM silver nitrate solution and 2.64 mL of 100 mM trisodium citrate solution into 41.25 mL of deionized water and stir evenly.

[0058] Step 2: Add 7.93mL, 50mM freshly prepared sodium borohydride solution into the mixed solution in step 1, and keep stirring for 12h. After stirring overnight, a silver nanoparticle solution was obtained.

[0059] Step 3, 307.3 mg of glutathione powder is added to the silver nanoparticle solution obtained in step 2, and then the mixture is reacted in an oil bath for 40 hours at 65° C. to obtain a light orange solution, which is stabilized with glutathione Silver nanoparticles solution.

[0060] Step 4, add 4 mL of the glutathione-stabilized silver nanoparticle solution of step 3 into 10.8 mL of deionized water, and stir evenly; then slowly add 1.2 mL, 10 mM chloroauric acid solutio...

Embodiment 3

[0064] The glutathione-gold-silver alloy nanomaterials prepared according to the methods of Example 1 and Example 2 were characterized by TEM, fluorescence, infrared, ultraviolet, XRD and SEM.

[0065] Such as figure 2 The three-dimensional fluorescence map of the glutathione-gold-silver alloy nanomaterial shown. It can be seen from the figure that the gold-silver alloy fluorescent nanomaterial prepared by the present invention has relatively strong fluorescence characteristics.

[0066] Such as image 3 and Figure 4 The TEM image and particle size distribution diagram of the glutathione-gold-silver alloy nanomaterials shown, in the case of glutathione as a stabilizer and surface protection agent, the gold-silver alloy nanomaterials are uniformly and stably distributed, and the particle size The diameter distribution is uniform, and the size is about 28.5nm.

[0067] Such as Figure 5 The optimal relationship diagram of fluorescence intensity of glutathione-gold-silver ...

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Abstract

The invention relates to a glutathione-Au / Ag alloy nano material, a preparation method and an application thereof. The preparation method includes following steps: 1) preparing a silver nano particle solution; 2) adding glutathione and performing an oil-bath reaction at 65-70 DEG C for 40-50 h, and adding deionized water with uniform stirring; 3) adding a chloroauric acid solution with stirring, centrifugally processing the mixed liquid, performing a mixed reaction to a supernatant with excessive methanol solution, and centrifugally processing the mixture again to obtain a precipitation, and 4) re-dissolving the precipitation in a PBS buffer solution to obtain a solution of the glutathione-Au / Ag alloy nano material. The glutathione-Au / Ag alloy nano material can be used in quantitative detection of histidine and immune-analysis of human chorionic gonadotropin. The preparation method is simple and convenient, wherein the glutathione is employed as a reduction agent and a stabilizer, so that the method is free of toxic reagents and is environment-friendly. The glutathione-Au / Ag alloy nano material is stable and uniform in structure, is good in dispersion property, is strong in fluorescence character, and can be used in analysis and detection of various bio-samples and detection of other relative functional materials.

Description

technical field [0001] The invention relates to the technical field of inorganic nanomaterials, in particular to a glutathione-gold-silver alloy nanomaterial and its preparation method and application. Background technique [0002] In the research of nanomaterials in recent years, noble metal nanomaterials have gradually shown their superiority in the detection of DNA, proteins, metal ions, hydrogen peroxide, toxic anions and thiols, and have attracted more and more researchers. more interest. But at the same time, a series of preparation methods, such as the use of polyols and microwave assistance, will be applied in the preparation of gold or silver-based nanomaterials. However, these preparation methods not only require high-temperature reactions, but also cause a lot of inconvenience because of the need to use microwave generators. Compared with the form of single metal components, multi-component nanomaterials such as gold-silver nano-alloy materials have the potentia...

Claims

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Application Information

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IPC IPC(8): C09K11/58B82Y30/00B22F9/24G01N21/64G01N33/76G01N33/68
Inventor 高文华黄晓鹏陈耀文陈汉佳张歆谢晓娜梁一丹
Owner SHANTOU UNIV
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