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Preparation method and application of flaxseed polysaccharides with antiviral and immune activity

An immune activity, flaxseed technology, applied in the field of biomedicine and functional food, can solve the problem of no sulfate group and triple helix advanced structure found in flaxseed polysaccharide, so as to improve the immune function of the body, improve the extraction efficiency, and improve the extraction efficiency. The effect of shortening time

Active Publication Date: 2015-11-11
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The extraction of traditional linseed polysaccharides mostly adopts hot water extraction method. The currently disclosed patents related to the extraction of linseed polysaccharides include: Chinese patent application publication numbers CN1221771, CN1242952, and CN1263139. These patents involve traditional hot water extraction. Improvement of extraction process of linseed gum, the extracted linseed polysaccharide has no sulfate group and triple helix advanced structure

Method used

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  • Preparation method and application of flaxseed polysaccharides with antiviral and immune activity
  • Preparation method and application of flaxseed polysaccharides with antiviral and immune activity
  • Preparation method and application of flaxseed polysaccharides with antiviral and immune activity

Examples

Experimental program
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Effect test

Embodiment 1

[0040] Embodiment 1: Preparation of linseed sulfate polysaccharide

[0041] Prepare the linseed sulfate polysaccharide as follows:

[0042] (1) Separation of linseed shell and kernel: after linseed is pulverized by a grinder, sieve, fully mix the sieved linseed powder with glycerin and ethanol, centrifuge for 20 minutes, collect the upper linseed kernel and the linseed deposited on the bottom layer respectively shell;

[0043] (2) Extraction of flaxseed polysaccharide: take 250g of flaxseed shell obtained in step (1) as raw material, the ratio of solid to liquid is 1:20 (g / mL), the extraction temperature is 60°C, the extraction time is 40min, the output power is 600W, Under the condition of a stirring speed of 600r / min, microwave-assisted hot water extraction of polysaccharides was carried out, the extract was passed through a 100-mesh sieve, the concentrated solution was collected, and vacuum freeze-dried;

[0044] (3) Separation of flaxseed polysaccharide: deproteinize the...

Embodiment 2

[0046] Embodiment 2: Preparation of linseed sulfate polysaccharide

[0047] Prepare the linseed sulfate polysaccharide as follows:

[0048] (1) Separation of linseed husk and kernel: After the linseed is pulverized by a grinder, sieve, fully mix the sieved linseed powder with glycerin and ethanol, and centrifuge for 30 minutes to collect the upper linseed kernel and the linseed deposited on the bottom layer respectively shell;

[0049] (2) Extraction of flaxseed polysaccharide: take 250g of flaxseed hull obtained in step (1) as raw material, the ratio of solid to liquid is 1:30 (g / mL), the extraction temperature is 100°C, the extraction time is 60min, the output power is 800W, Under the condition of a stirring speed of 900r / min, microwave-assisted hot water extraction of polysaccharides was carried out, the extract was passed through a 100-mesh sieve, the concentrated solution was collected, and vacuum freeze-dried;

[0050] (3) Separation of flaxseed polysaccharide: deprote...

Embodiment 3

[0052] Embodiment 3: Identification of the purity of linseed sulfate polysaccharide (FHP-1)

[0053] The purity of the linseed sulfate polysaccharide (FHP-1) obtained through separation and purification in Example 2 was determined by GPC. The specific test conditions and methods are as follows: TSKG-5000PWXL and TSKG-3000PWXL chromatographic columns are used in series with 2414 differential refractive index detectors, the column temperature is 35°C, and the mobile phase is 0.02mol / L KH 2 PO 4 , the flow rate is 0.6mL / min. Accurately weigh 2.0-2.5 mg of the sample, dissolve it in 1 mL of mobile phase, pass through a 0.22 μm filter membrane, manually inject 10 μL, and the GPC spectrum is as follows: Figure 4 shown. The purity of the polysaccharide component was judged according to the chromatogram, and the result showed that the purified FHP-1 was a polysaccharide with a uniform molecular weight, and the molecular weight was about 2626kD.

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Abstract

The invention discloses a preparation method and an application of flaxseed polysaccharides with antiviral and immune activity. The preparation method adopting flaxseeds as a raw material comprises the following steps: crushing flaxseeds, carrying out shell-kernel separation, carrying out microwave assisted hot water extraction, carrying out Sevage technology protein removal, carrying out ethanol precipitation, freeze-drying to obtain crude flaxseed polysaccharides, and carrying out ion exchange column chromatography, carrying out Sephadex column chromatography, and carrying out ultrafiltration to prepare flaxseed sulfated polysaccharides. FHP-1 obtained in the invention has homogeneous composition, and the molecular weight is 2626kDa. Polysaccharide with sulfate and triple-helical structure are separated from the flaxseeds in the invention for the first time. Cell biology experiments prove that the polysaccharides can reduce expression of hepatitis B surface antigen and hepatitis B e antigen and inhibit replication of hepatitis B viruses, can activate immune response, and improves secretion of the tumor necrosis factor TNF-a, interleukins IL-6 and IL-12, and an inflammation factor NO by immune cells.

Description

technical field [0001] The invention belongs to the fields of biomedicine and functional food, and in particular relates to the preparation and application of a flaxseed polysaccharide with antiviral and immune activities. Background technique [0002] Flax (Linumustitatissimum L.), also known as flax, belongs to the flax family and the genus flax. As a traditional oil crop, flaxseed is rich in unsaturated fatty acids, essential amino acids, vitamin E and sugar compounds and other nutrients, among which polysaccharides mainly exist in flaxseed hulls, and the content varies with the place of origin and variety rather different. [0003] Polysaccharide, also known as polysaccharide (Polysaccharide), is a polymer compound formed by connecting more than 10 monosaccharides through glycosidic bonds, and its relative molecular mass is tens of thousands to millions. Polysaccharides are widely distributed in nature and exist in higher plants, algae, fungi and animals, such as funga...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61P31/20A61P37/04
CPCA61K2236/00A61K31/715A23L33/125A61P37/04A61P31/20A61K36/55C08B37/0003Y02A50/30A23V2002/00
Inventor 汪勇李小凤廖文镇梁珊滕英来
Owner JINAN UNIVERSITY
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