Composition and preparing method and beauty preparation thereof
A composition and preparation technology, applied in the field of cosmetic plastic surgery, can solve the problems of single composition of cosmetic preparations and insufficient effect, and achieve the effects of improving wrinkle-removing function, diluting scars and stains, and promoting angiogenesis.
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[0037] The present invention also provides a preparation method of the above composition, comprising: mixing 30-70 parts by volume of cell growth factor solution, 30-70 parts by volume of high-concentration platelet plasma and 0.1×10 6 ~2×10 6 Adipose stem cells / mL were mixed to obtain a composition.
[0038] Wherein, the cell growth factor solution, high-concentration platelet plasma, and adipose stem cells are all the same as above, and will not be repeated here. The cell growth factor solution is obtained by mixing the freeze-dried cell growth factor powder with a solvent, and the solvent is a solvent well-known to those skilled in the art, and there is no special limitation. In the present invention, physiological saline is preferably used as the solvent.
[0039] According to the present invention, it is preferred to mix 30 to 70 parts by volume of high-concentration platelet plasma with 0.1×10 6 ~2×10 6 Adipose stem cells per mL are mixed, and then mixed with 30-70 pa...
Embodiment 1
[0046] 1.1 Take the adipose stem cells of the P1 generation with a confluence of 90%, remove the culture supernatant, wash with normal saline for 3 times, and add 0.02mL / cm 2 0.25% trypsin and 0.02% EDTA were digested for 2 minutes, and the complete medium (lonza UltraCULTUREMEDIUM serum-free medium + PALL serum substitute + 2mML-glutamine + 1xNEAA) 10 times that of the digestion solution was used to terminate the enzymolysis, and the samples were counted. Centrifuge at 200g for 5min, remove the supernatant, resuspend the cells with normal saline, adjust the total number of cells to 5×10 according to the counting results 6 Add normal saline to 45mL, centrifuge at 200g for 5min, wash the cells twice according to the above steps, and finally precipitate the prepared adipose stem cells.
[0047] 1.2 Take 20 mL of autologous whole blood and centrifuge at 400 g for 10 min, take plasma, discard red blood cells, centrifuge at 1200 g for 5 min, keep 2 mL of plasma, discard the rest, r...
Embodiment 2
[0050] 2.1 Take the adipose stem cells of the P2 generation with a confluence of 80%, remove the culture supernatant, wash with normal saline for 3 times, and add 0.02mL / cm 2 0.25% trypsin and 0.02% EDTA were digested for 2 minutes, and the complete medium (lonza UltraCULTUREMEDIUM serum-free medium + PALL serum substitute + 2mML-glutamine + 1xNEAA) 10 times that of the digestion solution was used to terminate the enzymolysis, and the samples were counted. Centrifuge at 200g for 5min, remove the supernatant, resuspend the cells with normal saline, and adjust the total number of cells to 0.5×10 according to the counting results. 6 Add normal saline to 45mLmL, centrifuge at 200g for 5min, wash the cells twice according to the above steps, and finally precipitate the prepared adipose stem cells.
[0051] 2.2 Take 20mL of autologous whole blood and centrifuge at 400g for 10min, get the plasma, discard the red blood cells, centrifuge at 1200g for 5min, keep 2.5mL of plasma, discard...
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