A kind of antimicrobial peptide and its application

A technology of antibacterial peptides and external preparations, applied in the direction of antibacterial drugs, antifungal agents, peptides, etc., to achieve the effect of easy acceptance by the human body and stable effect

Active Publication Date: 2018-06-19
JIANGSU PROTELIGHT PHARMA & BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present invention aims to provide an antimicrobial peptide and its application to solve the increasingly serious problem of drug resistance of bacteria and fungi and the pain caused by stubborn infection to the majority of patients

Method used

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  • A kind of antimicrobial peptide and its application
  • A kind of antimicrobial peptide and its application
  • A kind of antimicrobial peptide and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Related Antimicrobial Peptide Sequence Information Derived from SEQ ID NO:1

[0054] The polypeptide SEQ ID NO: 1 is composed of 11 amino acid residues, and the sequence is Ac-Lys D -Lys D -Leu D -Leu D -Phe D -Lys D -Leu D -Lys D -Phe D -Lys D -Lys D -NH 2 , an antimicrobial peptide derived from the NK sequence. SEQ ID NO: 1 is an amphipathic α-helical antimicrobial peptide with a polar surface and a nonpolar surface, which is used as a template polypeptide in the present invention. Its polar surface is composed of 6 hydrophilic amino acids (lysine). In contrast, its nonpolar surface consists of 5 hydrophobic amino acids (3 leucine, 2 phenylalanine).

[0055] The hydrophobic amino acids on the surface of the polypeptide constitute the overall hydrophobic surface of the polypeptide through hydrophobic interactions. The amino acid residues that make up NK, especially the hydrophobic amino acids, are recombined (it can be reorganized the hydrophobic amino ac...

Embodiment 2

[0066] Peptide analogs formed by substitution of similar hydrophobic amino acids

[0067] Further polypeptides of the present invention are polypeptide analogs formed by substitution of similar hydrophobic amino acid residues at a single site. Substituting a single hydrophobic amino acid with an amino acid having a similar hydrophobic side chain usually results in a biologically active polypeptide, see Table 3 (amino acid residues that can be used for homologous amino acid substitution).

[0068] table 3

[0069] Residues in SEQ ID NO: 1

Substituted Amino Acid Residues

Leu

Ala, Val, Lys

Phe

Leu, Ala, Lys

Lys

Phe, Val

[0070] Preparation of SEQ ID NO: 1 and related antibacterial peptides and detection of related parameters

[0071] All the polypeptides in the present invention are synthesized by using the polypeptide solid-phase synthesis method and using the N-fluorenylmethoxycarbonyl protection method. It should be pointe...

Embodiment 3

[0082] SEQ ID NO: 1 series antibacterial peptide antibacterial drug susceptibility test

[0083] 20 standard strains and 20 clinically isolated drug-resistant strains were selected to be kept in the laboratory, and the drug susceptibility test was carried out using the plate double dilution method and Denlay multi-point inoculator. The test bacteria were enriched with nutrient broth and brain heart infusion. After the drug is dissolved, dilute it twice with MH broth to obtain various required concentrations, and add an appropriate amount to the plate respectively. After the medium in the plate was solidified, the test bacteria were inoculated with a multi-point inoculator (10 4 CFU / point), and observe the results after incubation at a constant temperature of 35°C for 18 hours. The minimal inhibitory concentration (MIC) is the minimum concentration of drug contained in the aseptically grown plate. A lot of research work has shown that the enantiomeric polypeptides are consist...

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Abstract

The invention discloses an antibacterial peptide and its application. Wherein, the antimicrobial peptide has the amino acid sequence of SEQ ID NO: 1, and SEQ ID NO: 1 is Ac-LysD-LysD-LeuD-LeuD-PheD-LysD-LeuD-LysD-PheD-LysD-LysD-NH2. The antimicrobial peptide of the present invention can be prepared by artificial synthesis, that is, polypeptide solid-phase synthesis, and the antimicrobial peptide can be used as a polypeptide and related compounds of antimicrobial preparations to solve the increasingly serious problem of drug resistance of bacteria and fungi and the impact of stubborn infections on the majority of patients. caused pain. The antibacterial peptide of the present invention has stable antibacterial effect, is non-toxic, and is easy to be accepted by the human body. It can be applied to various stubborn infectious diseases and common infections, and can be used as an excellent substitute drug or auxiliary drug for existing antibiotics.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an antimicrobial peptide and its application. Background technique [0002] Antibiotics are drugs that can resist pathogenic microorganisms and are the largest category of antibacterial and anti-inflammatory drugs. Antibiotics are substances produced by bacteria, fungi or other microorganisms in the course of life, which can inhibit or kill bacteria, fungi, spirochetes, mycoplasma, chlamydia and other pathogenic microorganisms, so they are used as anti-infective drugs. There are also antibiotics to treat malignant tumors. Antibiotic drugs are widely used in various infectious diseases, and there are many varieties. [0003] At present, due to the abuse of antibiotics, some stubborn infections have caused great suffering to patients. Bacterial drug resistance is the phenomenon that bacteria are not sensitive to antibiotics, and the reason is a special form of expression of bacteria...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/06A61K38/08A61P31/04A61P31/10
Inventor 陈育新曲相如陈明侠白石琦黄宜兵
Owner JIANGSU PROTELIGHT PHARMA & BIOTECH
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