Anti-toxoplasma composition drug and screening method thereof

A screening method and technology for Toxoplasma gondii, applied in the field of medicine, can solve problems such as being difficult to completely cure, and achieve the effect of good anti-Toxoplasma gondii effect

Active Publication Date: 2015-10-28
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

(2) Drugs against folic acid metabolism: pyrimethamine is considered to be the most effective drug for the treatment of ocular toxoplasmosis; sulfadiazine can be used for adjuvant treatment of ocular toxoplasmosis; sulfamethoxazole combined with TMP can treat mice Experimental toxoplasmosis, to prevent the occurrence of chronic toxoplasmic encephalitis, but it is difficult to completely cure

Method used

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  • Anti-toxoplasma composition drug and screening method thereof
  • Anti-toxoplasma composition drug and screening method thereof
  • Anti-toxoplasma composition drug and screening method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The establishment of embodiment 1 mouse experiment toxoplasmosis animal model

[0033] The tachyzoites of PYS strain preserved in liquid nitrogen were resuscitated in mice for three consecutive generations, and SPF Kunming mice were inoculated into the abdominal cavity. After 72 hours of inoculation, the mice were killed by cervical dislocation, and the abdominal cavity was washed with an appropriate amount of normal saline, and the washing solution was taken out and diluted appropriately. Count with a white blood cell counter. Mice were infected with Toxoplasma gondii at a dose of 1 × 10 3 Tachyzoites / only, the ascites was diluted with normal saline to 5×10 3 tachyzoites / mL, each mouse was inoculated with 0.2 mL, and mixed before injection. The time of tachyzoites in vitro does not exceed 2h.

Embodiment 2

[0034] Embodiment 2 drug treatment scheme test one

[0035] (1) Experimental grouping: Experimental mice were randomly divided into 13 groups, including 10 drug groups, infection-untreated group (positive control group), injection blank control group and intragastric administration blank control group, with 26 mice in each group. The experimental observation period is 30 days.

[0036] (2) Infection dose: PYS strain tachyzoites 1×10 3 individual / mouse.

[0037] (3) For the test drug and administration method, see Table 1 :

[0038] Table 1 Drug grouping and administration method in trial 1

[0039]

Embodiment 3

[0040] The comparison of embodiment 3 test one each groups mouse survival time and protective rate

[0041] From the beginning of mouse infection, the survival of mice in each group was observed, and the death situation was recorded. The survival time of mice that survived for more than 30 days was counted as 30 days. For the average survival time and protection rate of each group of mice in Test 1, see Table 2 :

[0042] Table 2 Average survival time and protection rate of mice in test 1

[0043] group Average survival time (days) Protection rate (%) positive control group 8.07±0.25 a 0 a Blank control group 30.00±0.00 b 100 b group one 27.54±1.31 b 70 b group two 29.38±0.62 b 90 b group three 8.14±0.11 a 0 a group four 30.00±0.00 b 100 b group five 9.17±0.03 a 0 a group six 29.06±0.63 b 80 b group seven 17.62±2.88 c 30 a group eight 28.96±0.71 b 70 b group nine 9...

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Abstract

The invention discloses an anti-toxoplasma composition drug and a screening method thereof, and belongs to the field of medicine. According to the anti-toxoplasma composition drug and the screening method thereof, mouse test toxoplasmosis animal models are established, the ten drugs of a sulfadiazine sodium injection, a compound sulfamethoxazole, a lincomycin hydrochloride injection, a sulfamonomethoxine sodium injection, a florfenicol injection, acetylspiramycin, pyrimethamine, roxithromycin, artemisinin and radix sophorae flavescentis are selected out, two compositions which have the best anti-toxoplasma effect are screened out, one composition comprises the sulfamonomethoxine, the pyrimethamine and TMP, and the other composition comprises the compound sulfamethoxazole and the acetylspiramycin; animal models in mice and pigs are established, it is verified that the two compositions are both effective on the treatment of artificially infected toxplasmosis in pigs, and the treatment effect on the pigs is in accordance with that of the mouse models. By means of the anti-toxoplasma composition drug and the screening method thereof, it is proved that the the feasible method is achieved by utilizing the mouse test toxoplasmosis models to evaluate the efficacy of drugs on treating toxplasmosis in pigs, and meanwhile two anti-toxoplasma drug compositions with good effects are provided.

Description

technical field [0001] The invention belongs to the field of medicine, in particular to an anti-toxoplasma prescription medicine and a screening method thereof. Background technique [0002] Toxoplasmosis is a zoonosis caused by Toxoplasma gondii. It is a parasitic protozoan with worldwide distribution that can infect more than 200 species of animals including humans. Under normal conditions, Toxoplasma gondii infection is mostly recessive infection, but for immunodeficiency or immunosuppression, such as AIDS, organ transplantation, and malignant tumor patients, Toxoplasma gondii infection may be one of the causes of death. Pregnant women are more likely to be infected with Toxoplasma gondii. Toxoplasma gondii can be transmitted to the fetus through the placental barrier, leading to miscarriage of pregnant women, developmental deformities of infants and young children, mental retardation, encephalitis and meningitis. In animals, acute toxoplasmosis causes fever, dyspnea, a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00A61K36/489A61P33/02A61K31/7056A61K31/7048A61K31/635A61K31/63A61K31/505
Inventor 袁子国夏丽君张秀香李秀珍冯伟利林瑞庆吕琳吕淑媚文青元
Owner SOUTH CHINA AGRI UNIV
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