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Sulfated Lachnum extracellular polysaccharide and application thereof in anticoagulant drugs

A technology of Trichodia granulosa and sulfate esterification, which is applied in the direction of pharmaceutical formulations, extracellular fluid diseases, medical preparations containing active ingredients, etc., to achieve stable and strong effects

Inactive Publication Date: 2015-10-14
HEFEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In fact, heparin has side effects in clinical application, such as spontaneous bleeding and osteoporosis, etc.

Method used

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  • Sulfated Lachnum extracellular polysaccharide and application thereof in anticoagulant drugs
  • Sulfated Lachnum extracellular polysaccharide and application thereof in anticoagulant drugs
  • Sulfated Lachnum extracellular polysaccharide and application thereof in anticoagulant drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Preparation of exopolysaccharide from Trichodia granulosa YM281

[0026] Trichodia granulosa YM281 (the strain is derived from the literature "Purification, structure, lipid lowering and liver protecting effects of polysaccharide from Lachnum YM281", journal: Carbohydrate Polymers, ISSN: 0144-8617, publication date: 2013.10, page number: 922– 930) Seed solution is inoculated into a 100L fermenter for fermentation, and the stirring speed is 160r min -1 , the fermentation temperature is 26°C, and the fermentation time is 8 days. Fermentation medium: anhydrous glucose 30g·L -1 , yeast extract 5g·L -1 , MgSO 4 ·7H 2 O 1g L -1 , KH 2 PO 4 1g·L -1 , V B1 50mg·L -1 , natural pH. Concentrate the fermentation broth by rotary evaporation, ethanol precipitation with 3 times the volume of 95% ethanol for 24 hours, 4500r·min -1 Centrifuge for 8 minutes and redissolve in distilled water. The obtained crude polysaccharide was deproteinized, decolorized, dialyzed (di...

Embodiment 2

[0062] Processing of Exopolysaccharide from Sulfated Trichomonas YM281

[0063] 1. Tablet processing:

[0064] Mix 300-600g of Trichodia granulosa YM281 sulfated exopolysaccharide with 200-250g of pharmaceutical excipient diluent and 30-60g of glidant, then mix with 250-600mL of ethanol to obtain soft material, and sieve the soft material to obtain Wet granules, after drying, granulate and finally add 3-10g of lubricant, mix well and then compress into tablets to make 1000 tablets. Dextrin or dry starch is used as the diluent of pharmaceutical excipients; talcum powder or micropowder silica gel or microcrystalline cellulose is used as the glidant; magnesium stearate or zinc stearate is used as the lubricant.

[0065] The preferred example of tablet processing recommended by the present invention is: get 500 g of sulfated exopolysaccharides of Trichodia granulosa YM281, 250 g of diluent dextrin, and 40 g of glidant talc powder, mix them evenly, and then add 300 g -500mL of et...

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Abstract

The invention belongs to the field of medical immunity, and provides a sulfated Lachnum extracellular polysaccharide and application thereof in anticoagulant drugs. The sulfated Lachnum extracellular polysaccharide is prepared by carrying out sulfur trioxide-pyridine composite sulfation modification on Lachnum polysaccharide LEP-1 which is secreted and extracted from Lachnum YM281. The pharmacological experiment proves that the sulfated Lachnum extracellular polysaccharide has prolonging actions on mouse bleeding time (BT) and mouse coagulation time (CT) under the conditions of both low dose (30 mg / kg) and high dose (90 mg / kg), which indicates that the sulfated Lachnum extracellular polysaccharide has an anticoagulation action, thereby providing a new way for anticoagulation research.

Description

technical field [0001] The invention belongs to the field of medicine and immunization, and relates to a sulfated exopolysaccharide of Trichodia granulosa and its application in anticoagulant medicine. Background technique [0002] According to the World Health Organization, cardiovascular disease has become one of the main causes of death in the world in recent years. Atherosclerosis is an important basic medical record of cardiovascular diseases, and on this basis, intra-arterial thrombosis is an important cause of acute cardiovascular diseases. Reducing the formation of thrombus can reduce the occurrence of cardiovascular diseases. The formation of thrombus is the result of multiple factors such as the inner wall of the blood vessel, blood coagulation, anticoagulation and fibrinolysis system. Hypercoagulability is associated with complications such as thrombosis and thromboembolism. Hypercoagulable state (HCS), that is, prethrombotic state (PTS), is a pathological proce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61P7/02
Inventor 叶明陈天乐杜湛湛徐平王玉芬袁怀波
Owner HEFEI UNIV OF TECH
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