Method for extracting tea oil by demulsification based on aqueous enzymatic method

Abstract

The invention discloses a method for extracting tea oil by demulsification based on an aqueous enzymatic method. The method comprises the following steps: processing tea seed powder by microwaves for 60 to 120 seconds; adding distilled water at a material-liquid mass ratio of 1:4 to 1:8, pulping, and collecting upper-layer emulsified liquid; pulping the residual water phase and residues again, and collecting upper-layer emulsified liquid; combining the collected emulsified liquid; adding 0.5-6% of composite enzymatic hydrolysis liquid based on the total mass of the emulsified liquid, performing water bath enzymolysis, centrifuging, and taking upper-layer tea oil; adding an ethanol solution into the residual emulsified liquid, performing water bath treatment, centrifuging, and collecting upper-layer tea oil; combining the collected tea oil, an drying in vacuum. According to the method, the emulsified liquid is high in oil content, and the process is simple, novel and practical; the emulsified liquid is subjected to composite enzyme hydrolysis and ethanol demulsification, so that the enzymatic hydrolysis volume is greatly reduced, the enzyme consumption is greatly reduced, and the process is economic and efficient; microwaves, enzymatic hydrolysis and ethanol are adopted for assisting in oil extraction, so that natural active ingredients of the tea oil are better preserved, and the oil extraction process is safe and nutritive.

Description

technical field [0001] The invention belongs to the field of grain and oil processing. It relates to an extraction method of camellia oil. Background technique [0002] The extraction techniques of tea seed oil mainly include traditional pressing method, organic solvent leaching method, Soxhlet extraction method, supercritical extraction method, water substitution method and aqueous enzymatic method, etc. The pressing method and organic solvent leaching method are commonly used in industry. [0003] The residual oil in the pressing method is relatively high, and the residual oil rate of the cake is as high as 8-12%. The high-temperature treatment destroys the nutritional components, such as the serious denaturation of the protein in the cake, and the tea seed husk mixed in the pressing makes the cake contain a lot of lignin. High, difficult to use; solvent leaching method has safety problems such as large equipment investment and solvent residue in the product; supercritic...

AI Technical Summary

Problems solved by technology

[0003] The residual oil in the pressing method is relatively high, and the residual oil rate of the cake is as high as 8-12%. The high-temperature treatment destroys the nutritional components, such as the serious denaturation of the protein in the cake, and the tea seed husk mixed in the pressing makes the cake contain a lot of lignin. High, difficult to use; solvent leaching method has safety problems such as large equipment investment and solvent residue in the product; supercritical extraction method has problems such as high equipment investment, high production cost, and poor color; water method (water substitution method and water enzyme method) is a "safe, efficient and economical" oil extraction method that can replace organic solvents. The extracted camellia oil has high quality and good retention of active ingredients

However, the serious emulsification phenomenon in the process of water extraction leads to extremely low oil extraction rate, which is the "bottleneck" problem that restricts the water extraction of camellia oleifera seed oil. However, the amount of enzyme used for demulsification by water enzymatic method is too large and the cost is too high. High, therefore, the water extraction process needs further research and improvement