Preparation method for mother strain of wild dictyophora rubrovolvatain of Mount Fanjing

Abstract

The invention discloses a preparation method for a mother strain of wild dictyophora rubrovolvatain of Mount Fanjing. The preparation method includes taking talinum paniculatum, rice, glucose and agar as raw materials, and preparing a talinum paniculatum test tube bevel culture medium serving as a mother strain culture medium for standby application; collecting strain eggs of the wild dictyophora rubrovolvatain of the Mount Fanjing, wrapping and sealing each strain egg with a preservative film immediately, and taking the strain eggs to a laboratory; disinfecting the strain eggs with tap water, alcohol, sodium hypochlorite, sterile water and the like, cutting the strain eggs open, taking out strain stems, cutting the strain stems into a plurality of blocks with the side length of 0.5-1cm, and inoculating each mother strain culture medium test tube with one block, wherein each strain egg can be used for inoculation of 8-12 test tubes; culturing the inoculated test tubes at the temperature of 20 DEG C in a dark place for 45 days to obtain the mother strain of the wild dictyophora rubrovolvatain of the Mount Fanjing. The preparation method has the advantages that the talinum paniculatum substitutes for chemical reagents added into ordinary culture media, simplicity and convenience in operation, low cost and short cycle are achieved, chemical pollution is avoided, and mother strains of similar edible mushrooms such as dictyophora indusiata can be prepared.

Description

technical field [0001] The invention relates to the field of edible fungus breeding and production, in particular to a method for preparing the female species of the fanjingshan wild red-twirled bamboo fungus. Background technique [0002] Soil ginseng ( Talinum paniculatum (Jacq.) Gaertn.), also known as soil ginseng, false ginseng, ginseng vegetable, etc., is a perennial herb of the family Portulaca oleracea. In the leaves of ginseng, the oxalic acid content is high, rich in calcium, phosphorus, iron, ascorbic acid, thiamine and other ingredients; its root is similar to ginseng, contains carbohydrates, can be stewed or soaked in wine; Edible, also can be cold and dressed with sauce, fried food, and taste is fresh and tender. Moreover, native ginseng is an excellent Chinese herbal medicine, which has the effects of invigorating the spleen and moistening the lungs, relieving cough, and regulating menstruation. It is known as "Southern ginseng". Therefore, in order to dev...

AI Technical Summary

Problems solved by technology

Cultivated species refer to the multiplication of original species, mainly for multiplication, for production, to provide enough strains) and other research aspects such as cultivation, there are fewer varieties of Dictyophora, the use of chemical reagents, the need for fresh bamboo, waste of raw materials ( In the production of potato culture medium, the potato only takes its soup, and most of the potato dregs are discarded), the production process is relatively cumbersome (in the production of the medium, it needs to be filtered or the pH value is adjusted, etc.), which is also the reason for the high price of bamboo fungus ( 700 yuan / ㎏), therefore, exploring the domestication of wild species, breeding new varieties of Dictyophora, using non-polluting raw materials, using less or no chemical reagents, reducing production process links, reducing costs, etc., in the production of Dictyophora has important practical significance