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A tumor phototherapy agent based on cell membrane coating and its preparation method and application

A cell membrane and reagent technology, which is applied in the field of anti-tumor phototherapy reagents, can solve the problems of weakening the yield of singlet oxygen and phototherapeutic efficiency, and achieves the improvement of singlet oxygen yield and fluorescence brightness, good biocompatibility, and inhibition of self-efficacy. quenching effect

Active Publication Date: 2017-11-03
SOUTHEAST UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] However, due to the π-π stacking interaction between porphyrin molecules, it is easy to form aggregates in aqueous solution, and this self-quenching phenomenon greatly weakens the yield of singlet oxygen and the efficiency of phototherapy.

Method used

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  • A tumor phototherapy agent based on cell membrane coating and its preparation method and application
  • A tumor phototherapy agent based on cell membrane coating and its preparation method and application
  • A tumor phototherapy agent based on cell membrane coating and its preparation method and application

Examples

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Effect test

Embodiment 1

[0041] (1) Firstly, using glycol chitosan (glycol chitosan) and N-hydroxysuccinimide ester-polyethylene glycol 2000-methoxyl (NHS-PEG2000-OMe) as raw materials, synthesize PEG molecules to account for chitosan The percentage of sugar repeating units is 20% of the precursor, ie chitosan-20% PEG. Weigh 40mg of NHS-PEG2000-OMe and 20mg of ethylene glycol chitosan respectively, dissolve them in PBS buffer (pH 7.4, 50mM), mix quickly and uniformly, and react overnight at room temperature in a shaker. After the reaction, it was purified by dialysis with a dialysis bag with a molecular weight cut-off of 10k for 3 days, and finally freeze-dried in a lyophilizer to make chitosan-20% PEG.

[0042] (2) Subsequently, chitosan-20% PEG, protoporphyrin (PpIX), 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide (EDC) and N-hydroxysuccinyl Imine (NHS) was used as a raw material to synthesize a phototherapy agent in which PpIX molecules accounted for 4% of chitosan repeating units: chitosan-20%PEG-...

Embodiment 2

[0046] According to the method of Example 1, protoporphyrin is replaced by indocyanine green, adjust the consumption of each component, the percentage of synthetic PEG accounting for chitosan repeating unit number is 2%, and indocyanine green molecule accounts for chitosan repeating unit The percentage of the number is 0.2% of the reagent.

Embodiment 3

[0048] According to the method of Example 1, protoporphyrin is replaced by chlorin E6, the consumption of each component is adjusted, the percentage of synthetic PEG accounting for chitosan repeating unit number is 30%, and chlorin E6 molecule accounts for chitosan The percentage of repeating units is 2% of reagents.

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Abstract

The invention discloses a tumor phototherapy reagent based on cell membrane coating, and the reagent is a polymer chitosan whose side chain contains photosensitizer molecules and polyethylene glycol molecules. The invention also discloses a preparation method of the tumor phototherapy agent and its application in the preparation of a photosensitizer. Compared with the prior art, the reagent of the present invention has very low toxicity to cells without light, but its photodynamic toxicity is greatly improved compared with free porphyrin molecules under irradiation of a certain wavelength. Most importantly, the invention also effectively solves the self-quenching effect between porphyrin molecules, and realizes cell membrane imaging and photodynamic tumor therapy based on porphyrin molecules.

Description

technical field [0001] The invention relates to a tumor phototherapy reagent based on cell membrane coating and its preparation method and application, belonging to the field of anti-tumor phototherapy reagents. Background technique [0002] Cancer is a disease caused by the abnormal control of cell growth mechanism. It may appear in various tissues in the human body and invade the surrounding normal tissues to metastasize, becoming an important factor that endangers human life and health. Traditional cancer treatment methods include surgical resection, chemotherapy and radiation therapy, etc., but the resulting secondary damage is often greater than the cancer itself, so these treatment methods still need to be improved. Photodynamic therapy is a new type of adjuvant cancer therapy developed in recent years. It has attracted extensive attention due to its advantages of less damage to normal tissues, no accumulation of drug toxicity, and less side effects. Among them, the p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K41/00A61K47/60A61K47/61A61K49/00A61P35/00G01N21/64G01N1/30
Inventor 吴富根贾浩然
Owner SOUTHEAST UNIV
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