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Application of Poria cocos culture in trapping and killing termites and termite trapping and killing bait

A technology of cultures and traps, applied in applications, insecticides, biocides, etc., can solve the problem of no ideal subterranean termite traps, achieve the effect of ensuring the effect of killing termites and reducing the amount of use

Active Publication Date: 2017-10-24
INST OF ZOOLOGY GUANGDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no ideal subterranean termite trap in China

Method used

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  • Application of Poria cocos culture in trapping and killing termites and termite trapping and killing bait
  • Application of Poria cocos culture in trapping and killing termites and termite trapping and killing bait

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1: Extraction of Poria cocos pine sawdust culture extract.

[0016] Remove impurities from pine wood chips, add 6% sucrose water with equal mass fraction after drying to constant weight. After stirring evenly, put it into a high-temperature resistant polypropylene plastic bag to 2 / 3 capacity, put a bamboo tube with an inner diameter of 40mm and a height of 60mm at the mouth of the plastic bag, turn the polypropylene plastic film out and tie it tightly on the bamboo tube with gauze tape Put a cotton plug on it, and sterilize it in a sterilizing pot for 90 minutes. After the sterilizer is cooled to room temperature, take out the pine sawdust, insert the bacteria liquid of Wolfiporia cocos (Schw.Ryv.&Gibn) under aseptic conditions, and culture it at 22-28°C for 45 days. When body is covered with whole polypropylene plastic film bag, finish cultivating, thus obtain Poria cocos pine sawdust culture.Poria cocos pine sawdust culture is baked to constant weight and cal...

Embodiment 2

[0019] Example 2: Determination of the activity of the extract concentrate of Poria cocos pine sawdust culture on Copotic termites.

[0020] Make bottom plate with frosted glass, draw a circle respectively with normal hexane solvent, and the normal hexane dilution of the Poria cocos pine sawdust culture extract concentrate (acquired by the method of Example 1) diluted 6 times with normal hexane, normal hexane solvent In the inner circle, the diameter is 110mm, and the n-hexane dilution of the extract concentrate of the Poria cocos pine sawdust culture is in the outer circle, with a diameter of 150mm. Put 5 worker ants of Formosa termites in the center of the circle, and quickly cover them with a glass petri dish with a diameter of 70mm After the termite worker ants settle down, uncover the petri dish, let the termites move randomly, observe and record the termite worker ants' direction, and take half of the circle drawn by the n-hexane dilution of the extract concentrate of Por...

Embodiment 3

[0025] Embodiment 3: Determination of the weight loss rate of Poria cocos culture substrate and the active reaction value of Taiwan milk termites

[0026] According to the method of Example 1, Poria cocos was inoculated into pine sawdust with known constant weight, inoculated 20 bags in total, divided into 10 parts, and cultured at 25°C for 25, 30, 35, 40, 45, 50, 55, 60 respectively. , 65, 70 days, thus obtaining the Poria cocos pine sawdust culture. After the cultivation, part of the pine sawdust culture of Poria cocos was taken and dried to constant weight, and the weight loss rate of the pine sawdust was calculated. Obtain the extract concentrate of Poria cocos pine sawdust culture extract according to the method of Example 1 again, then measure respectively the activity of Taiwan milk termites to the normal hexane dilution of Poria cocos pine sawdust culture extract concentrate according to the method of embodiment 2 reaction, the measurement results show that the cultur...

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Abstract

The invention discloses an application of a wolfiporia cocos bacterial culture in termite trap-killing, and a termite trap-killing bait. The wolfiporia cocos bacterial culture and extract thereof have a significant trapping effect upon termites. The trapping effect exceeds those of previously reported other bacteria, rot fungi and culture extracts thereof. Therefore, the wolfiporia cocos bacterial culture and the extract thereof can be used for luring termites, such that termite intake can be accelerated, and food consumption can be increased. When the wolfiporia cocos bacterial culture is added into a termite trap-killing bait, the termite killing effect of the bait can be ensured, and dose can be reduced. The application also has an environment protection significance.

Description

Technical field: [0001] The invention belongs to the field of termite control, and in particular relates to the application of Poria cocos fungus culture in trapping and killing termites and a bait for trapping and killing termites. Background technique: [0002] Mirex belongs to Persistent Organic Pollutants (POPs), which has been banned all over the world. Although arsenic trioxide powder is a traditional agent for exterminating termites in my country, it cannot be registered in my country because of its toxicity. In my country, only 0.5% fipronil powder and 3% ivermectin powder are registered as termite-killing agents, but whether it is arsenic trioxide powder or 0.5% fipronil powder and 3% ivermectin powder, these Insecticide powders have a strong repellency to termites. The termites are basically driven or no longer pass through the ant road or ant-affected area covered by these insecticide powders. Therefore, these insecticide powders are used to kill termites. If the n...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/04A01P19/00A01P7/04A01N47/34
Inventor 钟俊鸿李秋剑肖维良刘炳荣曾文慧胡少芳黄海涛
Owner INST OF ZOOLOGY GUANGDONG ACAD OF SCI
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