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Glyphosate detection microfluidic chip, glyphosate antigen immobilization method and detection method

A microfluidic chip, fixing method technology, applied in chemical instruments and methods, biological testing, measuring devices, etc., can solve the problems of limited application, long antigen-antibody binding time, large consumption of samples and reagents, etc. High speed, suitable for on-site measurement, and high work efficiency

Active Publication Date: 2017-07-18
HANGZHOU GREAN WATER SCI & TECH INC
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, immunoassays are usually carried out on 96-well plates, the consumption of samples and reagents is large, and the combination time of antigen-antibody is long, these shortcomings limit its further application

Method used

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  • Glyphosate detection microfluidic chip, glyphosate antigen immobilization method and detection method
  • Glyphosate detection microfluidic chip, glyphosate antigen immobilization method and detection method

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Embodiment Construction

[0038] The implementation of the present invention will be described in detail below in conjunction with specific examples. It should be understood that the implementation of the present invention is not limited to the following examples, and any modifications and / or changes made to the present invention will fall within the protection scope of the present invention.

[0039] Such as figure 1 As shown, the microfluidic chip for glyphosate detection includes a substrate at the bottom and a cover sheet sealed on it, and a microfluidic channel is set on the substrate, and the microfluidic channel includes a derivatization channel and an immunoreaction detection connected to it Channel 5, glyphosate antigen is immobilized on the inner surface of the immune reaction detection channel 5, a water sample inlet 1 and a derivatization reagent inlet 8 are set at the entrance of the derivatization channel, and a waste liquid outlet 4 is set at the outlet of the immune reaction detection c...

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Abstract

The invention discloses a glyphosate detection micro-fluidic chip, which comprises a substrate located on the bottom part and a cover plate covered above the substrate in a sealing manner, wherein a micro-fluidic circuit is arranged on the substrate and comprises a derivatization channel and an immune response detection channel connected with the derivatization channel; a glyphosate antigen is fixed on the inner surface of the immune response detection channel; a water sample inlet and a derivative reagent inlet are arranged at an inlet of the derivatization channel; a waste liquid outlet is formed at an outlet of the immune response detection channel; a phosphate buffer solution inlet, a glyphosate antibody solution inlet, a Cy3 fluorescent dye labeled second antibody solution inlet and a sodium dodecyl sulfonate solution inlet are arranged close to the joint of the derivatization channel and the immune response detection channel. The invention also discloses a glyphosate antigen fixation method and a glyphosate detection method. The glyphosate detection micro-fluidic chip provided by the invention has the advantages of capability of simply, conveniently, quickly and accurately detecting glyphosate in water and the like.

Description

technical field [0001] The invention relates to a glyphosate detection microfluidic chip, a glyphosate antigen immobilization method and a detection method. Background technique [0002] Glyphosate (GLY) is used as a herbicide. Many studies in recent years have shown that glyphosate has certain toxicity to organisms. Glyphosate entering the environment will have harmful effects on the ecological environment, causing water pollution, vegetation destruction, and passing Residues in food pose a threat to human health and have adverse genetic effects. Therefore, the "Drinking Water Hygienic Standard" (GB 5749-2006) promulgated by my country in 2006 stipulates that the content of glyphosate in drinking water shall not exceed 0.700mg / L. The detection of glyphosate in "Standard Examination Methods for Drinking Water" (GB / T 5750—2006) adopts post-column derivatization ion exchange chromatography, adding automatic derivatization equipment after the chromatographic column, which is c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/543G01N33/58B01L3/00
CPCB01L3/00B01L3/5027G01N33/543G01N33/54306G01N33/54353G01N33/582G01N2430/20
Inventor 崔海松毛芳芳邹爽魏峰
Owner HANGZHOU GREAN WATER SCI & TECH INC
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