Method for continuously preparing raphanin

Abstract

The invention discloses a method for continuously preparing raphanin, and particularly relates to a method for preparing raphanin from radish seeds. The raphanin with the purity of over 98 percent is prepared by performing crushing, degreasing and hydrolysis on air-dried radish seeds serving as raw materials, mounting a raphanin continuous hydrolysis column and a raphanin continuous hydrolysis separation system and preparing raphanin liquid and a raphanin-enriched macroporous adsorption resin column through separation and freeze-drying. The raw materials are readily available, and the method is high in operability; equipment is easily obtained, and the technology is simple; the production process is safe, and industrial production is facilitated. A raphanin product with the activities of preventing and resisting cancers can be widely applied to the industries such as medicines and health products.

Description

1. Technical field [0001] The invention belongs to the technical field of separation and purification of natural active substances, and in particular relates to a preparation method of sulforaphane. 2. Background technology [0002] Epidemiological and pharmacological studies have shown that cruciferous vegetables contain active ingredients that prevent or slow down the development of cancer, such as broccoli, which is a variety of Brassica cabbage species with green curds as products. The seeds are rich in glucosinolates. Under the catalysis of glucosinosidase or acid, glucosinolates generate isothiocyanates such as sulforaphane. Sulforaphane is one of the strongest anti-cancer ingredients found in vegetables so far. It can induce the body to produce Phase II detoxification enzymes-glutathione transferase and quinone reductase, which can detoxify electrophilic carcinogens Interfering with its binding and damage to DNA, and inhibiting the production of Phase I enzymes, the...

AI Technical Summary

Problems solved by technology

The main problems of this method are: ① the seeds are not degreased, and the glucosinolate and glucosinosidase in broccoli seeds are difficult to effectively contact due to the blocking of oil, resulting in a low production rate of sulforaphane; Control the pH and temperature conditions of the hydrolyzate, and the hydrolysis of glucosinolate by glucosinosidase is in a non-optimized environment, resulting in the generation of a large amount of impurities, difficult separation and purification, and high cost; ③The selectivity of extraction with ethyl acetate is low , can not only extract sulforaphane, but also extract its analogs, and concentrate in a vacuum at a higher temperature, sulforaphane is unstable, easy to rearrange to generate multiple isomers, and the product purity is low; ④ recovery rate of absolute ethanol Low, high production cost, uncompetitive

The main problems of this method are: ① the water content of the edible part of fresh broccoli is as high as about 90%, the energy consumption for drying is extremely high and the sulforaphane is rapidly decomposed in a high temperature environment; There are a lot of impurities and miscellaneous enzymes in the crude enzyme liquid, and there are many impurities generated by hydrolysis, and the production rate of sulforaphane is low; ③ because the stability time of sulforaphane in aqueous solution is only about 90 minutes, and the hydrolysis time is as long as 8 hours, here During this period, a large amount of sulforaphane is rearranged or isomerized, resulting in an extremely low production rate of sulforaphane

It can be seen that the above-mentioned published invention patents have common defects such as extremely low sulforaphane production rate, long production time, high energy consumption, low product purity, and high cost. It is necessary to further develop new methods to improve the sulforaphane production rate and Purity, lower production costs