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Composite nanoparticle for controlling H2S release by employing near-infrared light as well as preparation method and application of composite nanoparticle

A composite nanoparticle and near-infrared light technology, applied in the field of biochemistry, can solve the problems of large tissue and cell damage, hinder application, and weak tissue penetration, and achieve the effect of strong penetration and little damage to cells and tissues.

Inactive Publication Date: 2015-06-03
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most existing organic H 2 S donors require UV light to generate H 2 S, ultraviolet light is very harmful to tissue cells and the tissue penetration is not strong, so it hinders this kind of H 2 Application of S donor in vivo

Method used

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  • Composite nanoparticle for controlling H2S release by employing near-infrared light as well as preparation method and application of composite nanoparticle
  • Composite nanoparticle for controlling H2S release by employing near-infrared light as well as preparation method and application of composite nanoparticle
  • Composite nanoparticle for controlling H2S release by employing near-infrared light as well as preparation method and application of composite nanoparticle

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Organic H 2 Chemical synthesis of the S donor molecule:

[0043] The synthetic route is as follows:

[0044]

[0045] The specific steps are as follows:

[0046] (1) Weigh 3g (0.0142mol) of Compound I and dissolve it in 15mL of anhydrous dichloromethane, and slowly add 7mL of it dropwise under ice-cooling to a concentration of 2molL -1 trimethylaluminum, the color of the solution changed from yellow to orange, and after 2 hours of reaction, the reaction solution was poured into hydrochloric acid diluted with ice water. The crude product was obtained by filtration, and the crude product was separated by column chromatography to obtain compound II. The yield was 50.7%.

[0047] (2) Weigh 1.63g of compound II and dissolve it in 15mL of dichloromethane, and stir in an ice bath. Measure 1.367mL of phosphorus tribromide and slowly add to the above solution. After reacting for 1.5h in ice bath, transfer to room temperature and continue to react for 1.5h. After the re...

Embodiment 2

[0052] Up-conversion nanoparticles UCNPs (β-LiYF4:0.5%Er3 + ,25%Yb3 + ) preparation:

[0053] The rare earth oxide (Y 2 o 3 :Yb 2 o 3 :Tm 2 o 3 =74.5%:25%:0.5%) was dissolved in an appropriate amount of trifluoroacetic acid, stirred and reacted at 80°C for 5h, and the trifluoroacetic acid was evaporated to obtain a white powder solid. This mixed powder was dissolved in a mixed solution of oleic acid and octadecene, and then 8 times the amount of lithium fluoride was added. Heated to 110° C. in a vacuum environment, and reacted for 1 h. Under nitrogen protection, raise the temperature to 320-330°C at 25°C / min, stir vigorously, react for 1 hour, and then cool to room temperature. An appropriate amount of acetone was added, and a white precipitate was obtained after centrifugation at 6000 rpm for 10 min. Dissolve in chloroform after washing twice with acetone and absolute ethanol. Centrifuge at 2000rpm for 1min to remove excess lithium fluoride, and finally obtain the ...

Embodiment 3

[0066] Determination of the number of SP molecules loaded on the surface of UCNPs:

[0067] (1) Drawing of UV standard curve: Prepare different concentrations of SP molecular solutions: 10μgmL -1 , 12.5 μg mL -1 , 16.5 μg mL -1 , 22.5 μg mL -1 , 28.5 μg mL -1 , 38.5 μg mL -1 , 50 μg mL -1 , measure the UV absorbance intensity, and draw the UV standard curve, see Figure 7 a.

[0068] (2) Take 0.25mL SP-PEG-UCNPs solution, mix it with 0.75mL acetonitrile solution, measure the UV absorbance intensity at 350nm, it is A 350nm = 0.093. Bring in the UV standard curve fitting function to get the concentration of SP molecules in this solution to be 7.71μgmL -1 . That is, there are 7.25×10 in 1mL SP-PEG-UCNPs solution 16 SP molecules.

[0069] (3) Take 1mL of pure UCNPs and dry and weigh it, which is 20mg. It is known that the volume of a UCNPs is 1.72×10 -16 cm 3 , the density of UCNPs is 4 gcm -1 , calculated to have 2.91×10 in 1mL solution 13 UCNPs.

[0070] (4) Th...

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Abstract

The invention discloses a composite nanoparticle for controlling H2S release by employing near-infrared light as well as a preparation method and an application of the composite nanoparticle. The composite nanoparticle is formed by loading organic H2S donor molecules on an up-conversion nanoparticle surface through a polyethylene glycol-oleylamine medium; and the preparation method is that the composite nanoparticle is obtained by chemical reaction of the up-conversion nanoparticle, the polyethylene glycol-oleylamine and the organic H2S donor molecules. The composite nanoparticle is good in stability; H2S can be released under near-infrared light illumination; the release rate can be regulated and controlled; the composite nanoparticle is good in cell compatibility, and small in cytotoxicity; the composite nanoparticle and the cell are cultured together, so that the donor cell, which can release the H2S under the near-infrared light illumination, and of which the release speed can be regulated and controlled can be obtained; and the composite nanoparticle is of great significance in the field of biological research.

Description

technical field [0001] The invention relates to a method of utilizing near-infrared light to control H 2 The novel composite nanoparticle released by S and its preparation method and application belong to the field of biochemistry. Background technique [0002] All the time, H 2 S is considered a poisonous gas. It not only has a strong irritating effect on the eyes and respiratory system of mammals, but if inhaled in large quantities, it may cause confusion, heart failure and other physiological reactions. However, recent studies have shown that H 2 S, like CO and NO, is a biological gas molecule, which has very important physiological significance. h 2 S has high reactivity with oxygen-containing substances and nitrogen-containing substances, such as hydrogen peroxide, peroxide, superoxide, nitroso, etc., and is an important antioxidant and scavenger in organisms. More importantly, H 2 S can undergo sulfhydryl reaction with cysteine ​​and connect to the iron center o...

Claims

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Application Information

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IPC IPC(8): C12N5/09C07C323/16C07C319/14
Inventor 邓留陈民陈万松刘又年
Owner CENT SOUTH UNIV
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