ospse1 Application of genes in regulating plant senescence

A technology of plant senescence and transgenic plants, applied in the field of genetic engineering, can solve the problems of shortening the grain filling time in the leaf functional period, limiting the improvement of crop yield and quality, and reducing the accumulation of dry matter, so as to achieve good application potential, delay plant senescence, Increase the effect of accumulation

Active Publication Date: 2020-08-07
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

During the period from heading to fruiting in rice, premature leaf senescence will shorten the leaf functional period and grain filling time, affect grain development, lead to reduced dry matter accumulation and ultimately limit the improvement of crop yield and quality

Method used

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  • <i>ospse1</i> Application of genes in regulating plant senescence
  • <i>ospse1</i> Application of genes in regulating plant senescence
  • <i>ospse1</i> Application of genes in regulating plant senescence

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1 rice senescence-related genes OsPSE1 / Ospse1 Construction of the electron physical map and screening of candidate genes

[0032] The present invention clones by map position cloning method OsPSE1 / Ospse1 Gene( figure 1 ).

[0033] The inventors obtained prematurely senescent rice plants during tissue culture Ospse1 , the inventor crossed the plant with the wild-type material CW312, and the obtained hybrid F 1 The phenotype of the generation plants was normal, indicating that the progeria gene was recessive relative to the normal gene. f 1 F 2 Generation population, count F from heading stage to fruiting stage 2 Number of wild-type and progeria individuals in population, progeria rice plants Ospse1 The segregation ratio with the wild-type material CW312 is 1:3, indicating that the premature aging phenotype is controlled by a pair of recessive genes.

[0034] prematurely senescent rice plants Ospse1 F 2 Separate groups for Ospse1 Genetic loca...

Embodiment 2

[0038] Example 2 OsPSE1 Genetic Complementation Vector and Overexpression Vector Transformed into Pregeriatric Plants of Rice Ospse1

[0039] According to the Nipponbare reference sequence, specific PCR primers including about 1 kb of the coding region and about 2.3 kb and 1.1 kb of the upstream and downstream regulatory regions were designed OsPSE1 -CT-F and OsPSE1 -CT-R (Table 2), using the high-fidelity enzyme KOD-Plus combined with long-range PCR (long-range PCR, LR-PCR) technology, using gDNA of wild-type CW312 as a template, amplified a target fragment of about 4.3 kb, and used restriction endonuclease Hin d III and Bam After HI cleavage, it was cloned into the plant binary vector pCAMBIA1300 (CAMBIA Company, Canberra, Australia) to obtain a genetic complementation vector.

[0040] Additionally designed to include candidate genes OsPSE1 Gene-specific primers for the entire ORF OsPSE1 -OE-F and OsPSE1 -OE-R (Table 2), PCR amplified CW312 genomic DNA to obtain a...

Embodiment 3

[0043] Embodiment 3 rice senescence-related genes OsPSE1 / Ospse1 Nucleotide sequence and protein structure

[0044] Amplified by PCR OsPSE1 and Ospse1 Genome sequence and sequencing, found rice premature senescence plant Ospse1 is in the wild type OsPSE1 A C base deletion occurred at the 947th bp of the coding region ( image 3 a), resulting in the extension of its coding region from 1095 bp to 1191 bp, and the encoded protein has a frameshift mutation from the point of mutation, OsPSE1 and Ospse1 The nucleotide sequences are shown in SEQ ID No: 1 and SEQ ID No: 3, respectively.

[0045] OsPSE1 and premature senescent plants Ospse1 The protein sequences are shown in SEQ ID No: 2 and SEQ ID No: 4, respectively. OsPSE1 It encodes a protein polypeptide consisting of 364 amino acid residues, with a molecular weight of about 39.5 kDa and an isoelectric point of 7.432; and Ospse1 It encodes a protein polypeptide consisting of 396 amino acid residues, with a molecular ...

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Abstract

The invention belongs to the technical field of gene engineering, and particularly discloses an application of an OsPSE1 gene in plant senescence regulation. According to the application of the OsPSE1 gene in plant senescence regulation, one premature aging rice plant is utilized, the OsPSE1 gene is cloned with a map-based cloning method, functional analysis is performed, and the result shows that the premature aging rice can return to normal through expression of the OsPSE1 gene in premature aging rice, and plant senescence can be further delayed and rice biomass accumulation is increased through overexpression of the OsPSE1 gene in normal rice; and the result proves that the OsPSE1 gene has better application potential in the aspect of increase of crop yield. The gene engineering means is utilized to regulate and control the expression level of the OsPSE1 gene for molecular breeding, and the OsPSE1 gene has potential application value in the aspect of increase of crop yield.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and specifically relates to OsPSE1 The use of genes in the regulation of plant senescence. Background technique [0002] Plant senescence is a process of gradual aging and death from the cell level to the tissue, organ and even the overall level of the plant determined by the growth period of the plant. It is the final stage of plant development. The senescence and death of plants are first manifested on the leaves, because leaves are an important place for plants to use light energy and synthesize organic compounds. The senescence of leaves will directly reduce the photosynthesis and biomass accumulation of crops. Therefore, leaves are an important phenotype for studying plant senescence. [0003] Plant senescence is not only regulated by the expression of internal genes, but also affected by the external environment of plant growth. In recent years, a variety of molecular cloning...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/82A01H5/00A01H6/46
Inventor 陈乐天刘耀光吴海斌韩靖鸾谢勇尧王斌陈远玲
Owner SOUTH CHINA AGRI UNIV
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