HPLC separate detection for polyphenols and rhodiola crenulata quality detection method
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A detection method, the technology of Rhodiola rosea, applied in the field of high-performance liquid chromatography detection, can solve the problems of limited quantity and difficulty in realizing the quality control of Rhodiola rosea medicinal materials, and achieve the effect of high practicality and applicability
Inactive Publication Date: 2015-04-29
西藏藏医药大学
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Problems solved by technology
The existing method can simultaneously separate and detect only 4 kinds of components in Rhodiola rosea, and the quantity is limited, so it is difficult to realize the comprehensive quality control of Rhodiola rosea
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Embodiment 1
[0020] like Figure 1 ~ Figure 2 As shown, the method of the present invention is used to detect the components of the Rhodiola rosea extract.
[0021] 1. Preparation of the test solution
[0022] Raw materials: take Rhodiola crenulata medicinal material, wash, dry and grind into powder for later use.
[0023] Take about 2.5g of raw medicinal material powder, weigh it accurately, put it in a 50mL measuring bottle, add 20mL of 30% ethanol, ultrasonicate at 20°C (power 250W, frequency 40kHZ) for 20min, let it stand at room temperature, add 30% ethanol to dilute to the mark, shake Evenly, accurately measure 5mL, put it in a 25mL measuring bottle, add 30% ethanol to dilute to the mark, shake evenly, filter, and take the subsequent filtrate to obtain the final product.
[0026] Reference substance solution A: take an appropria...
Embodiment 2
[0040] The components of the Rhodiola rosea extract were detected by the method of the present invention. The same parts as in Example 1 were not repeated, and the difference was in the gradient elution conditions. Gradient elution conditions are shown in Table 2. The chromatograms are shown in image 3 .
[0041] Table 2 gradient elution conditions
[0042]
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Abstract
The invention discloses an HPLC separate detection for polyphenols. Aiming at the defects of limited quantity of components, which can be simultaneously separated and detected, in a rhodiola crenulata medicinal material in the prior art, and poor comprehensive quality control effect on the rhodiola crenulata medicinal material, the invention provides a high performance liquid chromatography method for six polyphenol components comprising gallate, rhodioloside and the like, and application of the high performance liquid chromatography method in quality detection of the rhodiola crenulata medicinal material. A test article and / or a reference substance for the detection method disclosed by the invention comprise at least one of gallate, rhodioloside, butyl alcohol, catechinic acid, ethyl gallate and p-coumaric acid; the chromatographic conditions are that a C18 chromatographic column is adopted, and the column temperature is 25 DEG C; the mobile phase is a mixed solution of acetonitrile and 0.04% phosphoric acid solution; the volume percent of the acetonitrile is 7%-20%; and gradient elution is carried out. The invention further provides a rhodiola crenulata quality detection method. The HPLC method in the detection method disclosed by the invention has conventional chromatographic conditions; special treatment on samples is not required; and the practicability and the applicability are high.
Description
technical field [0001] The present invention relates to a high-performance liquid chromatography separation and quantitative detection method for polyphenol mixtures, in particular to a high-performance liquid chromatography detection method for six components including gallic acid and salidroside and its application . And the quality detection method of Rhodiola grandiflora. Background technique [0002] Rhodiola crenulata (Rhodiola crenulata), also known as Rhodiola broad-petal, Sedum broad-leaf, Sedum round, was included in the Chinese Pharmacopoeia for the first time in 1977. "The Pharmacopoeia of the People's Republic of China" (2005 edition) clearly stipulates that the medicinal material source of Rhodiola rosea is the dry root and rhizome of Rhodiola grandis. According to the public data of China Food and Drug Administration (CFDA), the relevant products that have been approved for marketing include 9 drugs containing Rhodiola rosea and 85 health foods containing Rh...
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