Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of cationic phospholipid-polymer nanoparticle and preparation method thereof

A nanoparticle and cationic liposome technology, which is applied in the field of cationic phospholipid-polymer nanoparticle and its preparation, can solve problems such as difficult loading of macromolecular plasmid DNA, and achieve good biodegradability, high endocytosis efficiency, and stable properties Effect

Active Publication Date: 2018-05-25
ZHUHAI INST OF ADVANCED TECH
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the above problems, the invention provides a cationic phospholipid-polymer nanoparticle, which contains cationic liposomes, which can absorb negatively charged DNA, and solve the problem of phospholipids in the prior art. -The problem that polymer nanoparticles are difficult to load macromolecular plasmid DNA; the present invention also provides a preparation method of cationic phospholipid-polymer nanoparticles, the preparation method is simple in process, easy to operate, and can be prepared on a large scale

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of cationic phospholipid-polymer nanoparticle and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] A preparation method of cationic phospholipid-polymer nanoparticles, comprising the following steps:

[0039] (1) Soybean lecithin and cationic liposome are dissolved in the ethanol aqueous solution that mass concentration is 4% with mass ratio as 1:4, obtain mixed solution, the total volume of mixed solution is 80mL, the concentration of soybean lecithin in mixed solution The concentration of the cationic liposome is 0.05mg / mL, and the cationic liposome is DOTAP; keep the mixed solution at a temperature of 60°C, adjust the pressure to 1400bar, and initially emulsify for 2min to obtain an emulsion;

[0040] (2) Dissolve the polymer PLGA with a molecular weight of 5000 in acetonitrile to obtain a polymer solution with a concentration of 1mg / mL, with a total volume of 20mL; drop the polymer solution into the emulsion, keep the temperature at 60°C, and keep the pressure 1400bar, continue to emulsify for 6min, and form 100mL solution containing cationic phospholipid-polymer...

Embodiment 2

[0044] A preparation method of cationic phospholipid-polymer nanoparticles, comprising the following steps:

[0045] (1) soybean lecithin and cationic liposome are dissolved in the ethanol aqueous solution that mass concentration is 4% with mass ratio as 1:3, obtain mixed solution, the total volume of mixed solution is 80mL, the concentration of soybean lecithin in mixed solution The concentration of the cationic liposome is 0.05 mg / mL, and the cationic liposome is DOTAP; keep the mixed solution at a temperature of 90°C, adjust the pressure to 500 bar, and initially emulsify for 5 minutes to obtain an emulsion;

[0046] (2) Dissolve polymer PLA with a molecular weight of 15000 in acetocyanide to obtain a polymer solution with a concentration of 1mg / mL, with a total volume of 20mL; drop the polymer solution into the emulsion, keep the temperature at 90°C, and keep the pressure 500bar, continue to emulsify for 4min, form 100mL the solution that contains cationic phospholipid-pol...

Embodiment 3

[0048] A preparation method of cationic phospholipid-polymer nanoparticles, comprising the following steps:

[0049] (1) Soybean lecithin and cationic liposome are dissolved in the ethanol aqueous solution that mass concentration is 4% with mass ratio as 1:5, obtain mixed solution, the total volume of mixed solution is 800mL, the concentration of soybean lecithin in mixed solution The concentration of cationic liposome is 0.05mg / mL, cationic liposome is 0.25mg / mL, and cationic liposome is DOTAP; keep the mixed solution at a temperature of 70°C, adjust the pressure to 1000bar, and initially emulsify for 1min to obtain an emulsion;

[0050] (2) Dissolve the polymer PLGA with a molecular weight of 10000 in acetone to obtain a polymer solution with a concentration of 1 mg / mL, with a total volume of 200 mL; add the polymer solution dropwise to the emulsion, keep the temperature at 70 °C and the pressure at 1000 bar , continue to emulsify for 5min to form 1000mL solution containing ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
concentrationaaaaaaaaaa
volumeaaaaaaaaaa
Login to View More

Abstract

The invention provides a cationic phospholipid-polymer nanoparticle, comprising an inner core and an outer shell, the inner core is a polymer, the outer shell is a cationic liposome and a phospholipid, and the cationic liposome and the phospholipid are coated on On the polymer surface, the polymer is polyglycolide lactide or polylactic acid. The cationic phospholipid-polymer nanoparticles have uniform particle size and stable properties, and can carry DNA into cells. The present invention also provides a preparation method of cationic phospholipid-polymer nanoparticles, which adopts a one-step high-pressure homogenization method to prepare cationic phospholipid-polymer nanoparticles, which is simple to operate and can prepare large doses of cationic phospholipid-polymer nanoparticles. The scale-up production and clinical research of the nanoparticles provide a basis, and the preparation method is simple and easy to implement and easy to popularize.

Description

technical field [0001] The invention relates to the field of drug carriers, in particular to a cationic phospholipid-polymer nanoparticle and a preparation method thereof. Background technique [0002] In recent years, the two mainstream nanocarriers represented by polymer nanoparticles and nanoliposomes can efficiently encapsulate and transport drugs, and have become a research hotspot for scientists from all over the world. Polymer nanoparticles have strong drug encapsulation ability, high cell endocytosis efficiency, and long circulation time in vivo. Nanoliposomes have the characteristics of excellent biological safety, high transport capacity, and simple preparation process. Phospholipid-polymer nanoparticles prepared by combining the respective advantages of polymers and phospholipids have been widely used in drug delivery. [0003] Gene therapy has brought hope for the effective prevention and treatment of some major diseases that have plagued human beings for a lon...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/127A61K47/34A61K47/24A61K48/00
Inventor 蔡林涛郑明彬陈泽罗震宇赵鹏飞龚萍
Owner ZHUHAI INST OF ADVANCED TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com