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Application of tetramycin P in inhibition of rhizoctonia solani kuhn of cucumbers and botrytis cinerea

A technology of Botrytis cinerea and Blight blight, which is applied in the field of microorganisms, can solve problems such as insecurity, and achieve a strong inhibitory effect

Inactive Publication Date: 2015-03-25
CHINA JILIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a new anti-phytopathogenic fungal active compound against the shortcomings of unsafe chemical pesticides and fewer types of microbial pesticides; another purpose of the present invention is to provide the control effect of the active compound on plant diseases

Method used

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  • Application of tetramycin P in inhibition of rhizoctonia solani kuhn of cucumbers and botrytis cinerea
  • Application of tetramycin P in inhibition of rhizoctonia solani kuhn of cucumbers and botrytis cinerea
  • Application of tetramycin P in inhibition of rhizoctonia solani kuhn of cucumbers and botrytis cinerea

Examples

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Effect test

Embodiment 1

[0020] Example 1: (Isolation and Screening of Amylase Streptomyces Chromogenes D)

[0021] The present invention obtained amylase chromogenic streptomyces ( Streptomyces diastatochromogenes ) D, save.

[0022] Amylase Streptomyces chromogenes ( S. diastatochromogenes ) Determination of antibacterial activity of D: Take 1 mL of the fermentation supernatant in a sterile petri dish, and quickly mix it with 9 mL of PDA medium cooled to 50°C. After cooling, put 1 diameter Cucumber solanum blight or tomato Botrytis cinerea with a thickness of 4 mm was tested. The cake was connected to the center of the petri dish (the diameter of the petri dish was 9 cm), and cultured in an incubator at 28°C for 36 h. The treatment was repeated 3 times as a control; the colony diameter was determined by the cross method, and the inhibition rate was calculated by the following formula:

[0023]

[0024] The results showed that the inhibition rate of amylase Streptomyces chromogenes D metaboli...

Embodiment 2

[0025] Embodiment 2: (preparation method of amylase Streptomyces chromogenes D fermentation metabolites)

[0026] Follow these steps:

[0027] (1) The slant surface activation culture of the strain test tube: the slant medium is Gaoshi No. 1 medium, and its composition and content are: K 2 HPO 4 0.5 g, KNO 3 1 g, MgSO 4 0.5 g, 20 g soluble starch, 0.5 g NaCl, FeSO 4 0.01 g, 20 g of agar, after dissolving, make up water to 1L; pick a little amylase spores of Streptomyces chromogenicum D with an inoculation loop under aseptic conditions, and put them into the sterilized Gaoshi No. 1 medium test tube (15× 150 mm), put it in an incubator at 28°C±1°C, activate and culture for 96 h, until the inclined surface of the test tube is covered with spores;

[0028] (2) Fermentation culture: The fermentation medium contains 20 g of soluble starch per 1000 mL, 40 g of soybean flour, NH 4 Cl 3 g, CaCO 3 5 g, MgSO 4 2 g, the balance is water; 60 mL of fermentation broth per 300 m...

Embodiment 3

[0030] Embodiment 3: (tetramycin P is to the inhibition of cucumber solanum blight or tomato Botrytis cinerea)

[0031] (1) Prepare tetramycin P solutions of various concentrations;

[0032] (2) Determination of antibacterial activity: Take 1 mL of tetramycin P solution of each concentration in a sterile petri dish, and quickly mix it with 9 mL of PDA medium cooled to 50°C, and place on each medium plane after cooling. Put a 4 mm diameter bacterial cake of cucumber solanum blight or tomato cinerea cinerea, connect the bacterial cake to the center of the petri dish (the diameter of the petri dish is 9 cm), put it in an incubator for 36 h at 28°C, and use conventional chemical The medicament and sterile water treatment were used as controls, repeated 3 times; the colony diameter was measured by the cross method, and the inhibition rate was calculated by the following formula:

[0033]

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Abstract

The invention discloses an application of tetramycin P in inhibition of rhizoctonia solani kuhn of cucumbers and botrytis cinerea, belonging to the microbial technical field. An active compound tetramycin P is separated and purified by virtue of extraction and chromatography according to a conventional chemical method by carrying out activated culture of a strain, fermental cultivation and fermentation broth collection on streptomyces diastatochromogenes D which is separated and screened from a soil sample which is collected in Tianmu Mountains, Linan, Hangzhou, Zhejiang province. The compound is a novel tetar-alkene macrolide antibiotic, can be used for inhibiting growth of pathogenic fungi of crops such as rhizoctonia solani kuhn of cucumbers and botrytis cinerea and is stronger in inhibitory effect than a common chemical agent.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a preparation method of a new compound tetramycin P and its inhibitory effect on plant pathogenic fungi. Background technique [0002] Among the crop diseases caused by different pathogenic bacteria, about 70% of the diseases are caused by fungi. Polyene macrolide antibiotics are a class of antibiotics with multiple conjugated double bonds, and their ultraviolet absorption is finger-like, which is very distinctive. According to the number of conjugated double bonds, polyene antibiotics can be divided into five types: triene, tetraene, pentacene, hexaene, and heptacene. They usually have strong antifungal activity and a wide antifungal spectrum. The main pathogenic fungi such as Cucumber solani ( Rhizoctonia solani ), Botrytis cinerea ( Botrytis cinerea ), Tomato early blight ( Alternayia solani ), Fusarium scab ( Fusarium graminearium ) and Fusarium wilt of cucumbe...

Claims

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Application Information

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IPC IPC(8): A01N43/90A01P3/00C12P19/62C12R1/525
Inventor 申屠旭萍俞晓平刘楠楠郝培应王正亮刘光富许益鹏
Owner CHINA JILIANG UNIV
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